Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2048 and SO2049 are separated by 225 nucleotides SO2049 and SO2050 are separated by 167 nucleotides
SO2048: SO2048 - membrane protein, putative (NCBI ptt file), at 2,147,446 to 2,149,071
SO2048
SO2049: SO2049 - GGDEF family protein (NCBI ptt file), at 2,149,297 to 2,150,256
SO2049
SO2050: SO2050 - conserved hypothetical protein (NCBI ptt file), at 2,150,424 to 2,151,068
SO2050
Position (kb)
2149
2150
2151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2148.334 kb on + strand, within SO2048 at 2148.354 kb on - strand, within SO2048 at 2148.415 kb on + strand, within SO2048 at 2148.417 kb on + strand, within SO2048 at 2148.427 kb on - strand, within SO2048 at 2148.447 kb on - strand, within SO2048 at 2148.545 kb on - strand, within SO2048 at 2148.674 kb on + strand, within SO2048 at 2148.682 kb on - strand, within SO2048 at 2148.684 kb on - strand, within SO2048 at 2148.684 kb on - strand, within SO2048 at 2148.758 kb on - strand, within SO2048 at 2148.862 kb on - strand, within SO2048 at 2149.477 kb on - strand, within SO2049 at 2149.612 kb on + strand, within SO2049 at 2149.622 kb on + strand, within SO2049 at 2149.789 kb on - strand, within SO2049 at 2149.886 kb on + strand, within SO2049 at 2150.196 kb on - strand at 2150.203 kb on + strand at 2150.209 kb on + strand at 2150.217 kb on - strand at 2150.228 kb on - strand at 2150.235 kb on + strand at 2150.235 kb on + strand at 2150.243 kb on - strand at 2150.323 kb on + strand at 2150.372 kb on + strand at 2150.497 kb on + strand, within SO2050 at 2150.641 kb on + strand, within SO2050 at 2150.649 kb on - strand, within SO2050 at 2150.666 kb on - strand, within SO2050 at 2150.732 kb on - strand, within SO2050 at 2150.766 kb on - strand, within SO2050 at 2150.774 kb on + strand, within SO2050 at 2150.782 kb on - strand, within SO2050 at 2150.839 kb on + strand, within SO2050 at 2150.847 kb on - strand, within SO2050 at 2150.891 kb on - strand, within SO2050 at 2151.015 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,148,334 + SO2048 0.55 -3.1 2,148,354 - SO2048 0.56 -0.2 2,148,415 + SO2048 0.60 -1.6 2,148,417 + SO2048 0.60 -2.9 2,148,427 - SO2048 0.60 -1.1 2,148,447 - SO2048 0.62 -1.2 2,148,545 - SO2048 0.68 -0.2 2,148,674 + SO2048 0.76 -3.4 2,148,682 - SO2048 0.76 -1.3 2,148,684 - SO2048 0.76 -1.8 2,148,684 - SO2048 0.76 -0.2 2,148,758 - SO2048 0.81 -3.4 2,148,862 - SO2048 0.87 -1.1 2,149,477 - SO2049 0.19 -1.6 2,149,612 + SO2049 0.33 -1.9 2,149,622 + SO2049 0.34 +1.1 2,149,789 - SO2049 0.51 +0.2 2,149,886 + SO2049 0.61 -1.3 2,150,196 - -0.8 2,150,203 + +1.8 2,150,209 + -1.0 2,150,217 - -0.3 2,150,228 - +1.8 2,150,235 + +0.5 2,150,235 + -3.6 2,150,243 - +1.1 2,150,323 + +1.7 2,150,372 + -0.7 2,150,497 + SO2050 0.11 -2.4 2,150,641 + SO2050 0.34 -1.4 2,150,649 - SO2050 0.35 -0.3 2,150,666 - SO2050 0.38 +0.3 2,150,732 - SO2050 0.48 -0.3 2,150,766 - SO2050 0.53 -0.2 2,150,774 + SO2050 0.54 +2.4 2,150,782 - SO2050 0.56 +1.6 2,150,839 + SO2050 0.64 +0.6 2,150,847 - SO2050 0.66 +1.2 2,150,891 - SO2050 0.72 +2.4 2,151,015 + -1.1
Or see this region's nucleotide sequence