Strain Fitness in Shewanella oneidensis MR-1 around SO2017

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthtpG and SO2017 are separated by 119 nucleotidesSO2017 and adk are separated by 140 nucleotidesadk and hemH-1 are separated by 79 nucleotides SO2016: htpG - heat shock protein HtpG (NCBI ptt file), at 2,114,424 to 2,116,337 htpG SO2017: SO2017 - hypothetical thioredoxin-like protein (NCBI ptt file), at 2,116,457 to 2,117,320 SO2017 SO2018: adk - adenylate kinase (NCBI ptt file), at 2,117,461 to 2,118,105 adk SO2019: hemH-1 - ferrochelatase (NCBI ptt file), at 2,118,185 to 2,119,231 hemH-1 Position (kb) 2116 2117 2118Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2115.760 kb on + strand, within htpGat 2115.995 kb on + strand, within htpGat 2116.414 kb on - strandat 2116.748 kb on + strand, within SO2017at 2116.748 kb on + strand, within SO2017at 2116.826 kb on + strand, within SO2017at 2116.834 kb on - strand, within SO2017at 2116.891 kb on - strand, within SO2017at 2116.972 kb on - strand, within SO2017at 2117.143 kb on + strand, within SO2017at 2117.143 kb on + strand, within SO2017at 2117.151 kb on - strand, within SO2017at 2117.168 kb on + strand, within SO2017at 2117.177 kb on + strand, within SO2017at 2117.299 kb on + strandat 2117.300 kb on - strandat 2117.307 kb on - strandat 2118.092 kb on - strandat 2118.153 kb on + strandat 2118.201 kb on + strandat 2118.289 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,115,760 + htpG SO2016 0.70 -2.6
2,115,995 + htpG SO2016 0.82 -1.8
2,116,414 - +0.5
2,116,748 + SO2017 0.34 -1.7
2,116,748 + SO2017 0.34 -2.8
2,116,826 + SO2017 0.43 +0.9
2,116,834 - SO2017 0.44 +1.5
2,116,891 - SO2017 0.50 -1.7
2,116,972 - SO2017 0.60 -0.0
2,117,143 + SO2017 0.79 -2.1
2,117,143 + SO2017 0.79 -0.6
2,117,151 - SO2017 0.80 -2.5
2,117,168 + SO2017 0.82 -2.7
2,117,177 + SO2017 0.83 -1.6
2,117,299 + +0.0
2,117,300 - -0.6
2,117,307 - -0.3
2,118,092 - -4.1
2,118,153 + -0.0
2,118,201 + +1.0
2,118,289 - +0.8

Or see this region's nucleotide sequence