Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1997 and SO1998 are separated by 270 nucleotides SO1998 and SO1999 are separated by 80 nucleotides SO1999 and ushA are separated by 363 nucleotides
SO1997: SO1997 - peptidase, M1 family (NCBI ptt file), at 2,096,495 to 2,098,381
SO1997
SO1998: SO1998 - conserved hypothetical protein (NCBI ptt file), at 2,098,652 to 2,098,879
SO1998
SO1999: SO1999 - phospholipase/carboxylesterase family protein (NCBI ptt file), at 2,098,960 to 2,099,625
SO1999
SO2001: ushA - 5'-nucleotidase (NCBI ptt file), at 2,099,989 to 2,101,698
ushA
Position (kb)
2098
2099
2100 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2098.041 kb on + strand, within SO1997 at 2098.049 kb on - strand, within SO1997 at 2098.049 kb on - strand, within SO1997 at 2098.161 kb on + strand, within SO1997 at 2098.169 kb on - strand, within SO1997 at 2098.188 kb on + strand, within SO1997 at 2098.210 kb on + strand at 2098.243 kb on + strand at 2098.291 kb on - strand at 2098.311 kb on - strand at 2098.522 kb on + strand at 2098.524 kb on - strand at 2098.549 kb on - strand at 2098.549 kb on - strand at 2098.557 kb on - strand at 2098.634 kb on + strand at 2098.637 kb on - strand at 2098.642 kb on - strand at 2098.644 kb on + strand at 2098.680 kb on - strand, within SO1998 at 2098.680 kb on - strand, within SO1998 at 2098.718 kb on - strand, within SO1998 at 2098.827 kb on + strand, within SO1998 at 2098.866 kb on - strand at 2099.066 kb on - strand, within SO1999 at 2099.066 kb on - strand, within SO1999 at 2099.120 kb on - strand, within SO1999 at 2099.136 kb on + strand, within SO1999 at 2099.144 kb on - strand, within SO1999 at 2099.180 kb on + strand, within SO1999 at 2099.193 kb on + strand, within SO1999 at 2099.254 kb on + strand, within SO1999 at 2099.282 kb on + strand, within SO1999 at 2099.303 kb on - strand, within SO1999 at 2099.335 kb on + strand, within SO1999 at 2099.336 kb on - strand, within SO1999 at 2099.363 kb on + strand, within SO1999 at 2099.371 kb on + strand, within SO1999 at 2099.381 kb on + strand, within SO1999 at 2099.418 kb on - strand, within SO1999 at 2099.418 kb on - strand, within SO1999 at 2099.474 kb on - strand, within SO1999 at 2099.474 kb on - strand, within SO1999 at 2099.554 kb on - strand, within SO1999 at 2099.600 kb on + strand at 2100.036 kb on - strand at 2100.054 kb on - strand at 2100.097 kb on - strand at 2100.222 kb on + strand, within ushA at 2100.360 kb on + strand, within ushA at 2100.514 kb on + strand, within ushA at 2100.516 kb on - strand, within ushA at 2100.529 kb on - strand, within ushA at 2100.624 kb on + strand, within ushA at 2100.624 kb on + strand, within ushA at 2100.624 kb on + strand, within ushA at 2100.624 kb on + strand, within ushA at 2100.624 kb on + strand, within ushA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,098,041 + SO1997 0.82 -1.6 2,098,049 - SO1997 0.82 -0.2 2,098,049 - SO1997 0.82 +0.3 2,098,161 + SO1997 0.88 -3.0 2,098,169 - SO1997 0.89 -0.6 2,098,188 + SO1997 0.90 -1.1 2,098,210 + +0.1 2,098,243 + +1.3 2,098,291 - +0.1 2,098,311 - -1.8 2,098,522 + +2.0 2,098,524 - +0.5 2,098,549 - +1.7 2,098,549 - +0.1 2,098,557 - +0.5 2,098,634 + -1.7 2,098,637 - -1.5 2,098,642 - -2.1 2,098,644 + +1.3 2,098,680 - SO1998 0.12 -1.2 2,098,680 - SO1998 0.12 -0.5 2,098,718 - SO1998 0.29 +0.2 2,098,827 + SO1998 0.77 -2.5 2,098,866 - -1.5 2,099,066 - SO1999 0.16 -0.4 2,099,066 - SO1999 0.16 +0.5 2,099,120 - SO1999 0.24 +1.1 2,099,136 + SO1999 0.26 -1.4 2,099,144 - SO1999 0.28 -3.6 2,099,180 + SO1999 0.33 +1.2 2,099,193 + SO1999 0.35 -0.6 2,099,254 + SO1999 0.44 -2.5 2,099,282 + SO1999 0.48 +0.6 2,099,303 - SO1999 0.52 +2.5 2,099,335 + SO1999 0.56 +1.7 2,099,336 - SO1999 0.56 -1.4 2,099,363 + SO1999 0.61 -2.4 2,099,371 + SO1999 0.62 -3.7 2,099,381 + SO1999 0.63 -2.6 2,099,418 - SO1999 0.69 -1.1 2,099,418 - SO1999 0.69 -1.8 2,099,474 - SO1999 0.77 -2.1 2,099,474 - SO1999 0.77 -0.6 2,099,554 - SO1999 0.89 -2.8 2,099,600 + -0.5 2,100,036 - +0.8 2,100,054 - +0.1 2,100,097 - -0.5 2,100,222 + ushA SO2001 0.14 -1.0 2,100,360 + ushA SO2001 0.22 -0.1 2,100,514 + ushA SO2001 0.31 -0.2 2,100,516 - ushA SO2001 0.31 +1.0 2,100,529 - ushA SO2001 0.32 +1.7 2,100,624 + ushA SO2001 0.37 -0.8 2,100,624 + ushA SO2001 0.37 -2.1 2,100,624 + ushA SO2001 0.37 -0.5 2,100,624 + ushA SO2001 0.37 -0.7 2,100,624 + ushA SO2001 0.37 +0.9
Or see this region's nucleotide sequence