Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoP and SO1947 are separated by 181 nucleotides SO1947 and SO1948 are separated by 165 nucleotides SO1948 and SO1949 are separated by 220 nucleotides
SO1946: phoP - transcriptional regulatory protein PhoP (NCBI ptt file), at 2,049,116 to 2,049,790
phoP
SO1947: SO1947 - hypothetical protein (NCBI ptt file), at 2,049,972 to 2,050,316
SO1947
SO1948: SO1948 - sodium:dicarboxylate symporter family protein (NCBI ptt file), at 2,050,482 to 2,051,795
SO1948
SO1949: SO1949 - invasin domain protein (NCBI ptt file), at 2,052,016 to 2,055,729
SO1949
Position (kb)
2050
2051
2052 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2049.514 kb on + strand, within phoP at 2049.559 kb on + strand, within phoP at 2049.601 kb on + strand, within phoP at 2049.709 kb on + strand, within phoP at 2049.824 kb on - strand at 2049.908 kb on - strand at 2050.057 kb on + strand, within SO1947 at 2050.057 kb on + strand, within SO1947 at 2050.067 kb on + strand, within SO1947 at 2050.249 kb on - strand, within SO1947 at 2050.314 kb on - strand at 2050.345 kb on + strand at 2050.429 kb on + strand at 2050.434 kb on + strand at 2050.614 kb on - strand, within SO1948 at 2050.866 kb on - strand, within SO1948 at 2050.900 kb on - strand, within SO1948 at 2050.946 kb on - strand, within SO1948 at 2050.994 kb on + strand, within SO1948 at 2051.057 kb on + strand, within SO1948 at 2051.099 kb on + strand, within SO1948 at 2051.182 kb on + strand, within SO1948 at 2051.196 kb on + strand at 2051.205 kb on - strand, within SO1948 at 2051.273 kb on + strand, within SO1948 at 2051.341 kb on + strand, within SO1948 at 2051.349 kb on - strand, within SO1948 at 2051.361 kb on + strand, within SO1948 at 2051.385 kb on - strand, within SO1948 at 2051.385 kb on - strand, within SO1948 at 2051.395 kb on - strand, within SO1948 at 2051.457 kb on + strand, within SO1948 at 2051.465 kb on - strand, within SO1948 at 2051.468 kb on + strand, within SO1948 at 2051.524 kb on + strand, within SO1948 at 2051.719 kb on - strand at 2051.719 kb on - strand at 2051.965 kb on + strand at 2051.996 kb on + strand at 2052.004 kb on - strand at 2052.015 kb on + strand at 2052.054 kb on - strand at 2052.054 kb on - strand at 2052.087 kb on - strand at 2052.087 kb on - strand at 2052.087 kb on - strand at 2052.087 kb on - strand at 2052.087 kb on - strand at 2052.122 kb on + strand at 2052.130 kb on - strand at 2052.205 kb on + strand at 2052.213 kb on + strand at 2052.213 kb on + strand at 2052.213 kb on + strand at 2052.213 kb on + strand at 2052.213 kb on + strand at 2052.213 kb on - strand at 2052.221 kb on - strand at 2052.233 kb on - strand at 2052.354 kb on + strand at 2052.377 kb on - strand at 2052.425 kb on + strand, within SO1949 at 2052.441 kb on + strand, within SO1949 at 2052.451 kb on + strand, within SO1949 at 2052.451 kb on + strand, within SO1949 at 2052.459 kb on - strand, within SO1949 at 2052.502 kb on + strand, within SO1949 at 2052.509 kb on - strand, within SO1949 at 2052.598 kb on - strand, within SO1949 at 2052.628 kb on + strand, within SO1949 at 2052.636 kb on - strand, within SO1949 at 2052.716 kb on + strand, within SO1949
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,049,514 + phoP SO1946 0.59 -3.9 2,049,559 + phoP SO1946 0.66 +0.1 2,049,601 + phoP SO1946 0.72 -3.8 2,049,709 + phoP SO1946 0.88 -3.4 2,049,824 - -0.7 2,049,908 - -0.3 2,050,057 + SO1947 0.25 +1.5 2,050,057 + SO1947 0.25 -0.4 2,050,067 + SO1947 0.28 +0.7 2,050,249 - SO1947 0.80 +0.9 2,050,314 - +0.1 2,050,345 + +1.0 2,050,429 + +0.5 2,050,434 + +0.4 2,050,614 - SO1948 0.10 -0.8 2,050,866 - SO1948 0.29 +0.5 2,050,900 - SO1948 0.32 -1.6 2,050,946 - SO1948 0.35 -1.8 2,050,994 + SO1948 0.39 -3.6 2,051,057 + SO1948 0.44 -0.1 2,051,099 + SO1948 0.47 -1.8 2,051,182 + SO1948 0.53 -0.6 2,051,196 + -0.8 2,051,205 - SO1948 0.55 -4.6 2,051,273 + SO1948 0.60 -3.4 2,051,341 + SO1948 0.65 -2.2 2,051,349 - SO1948 0.66 -1.9 2,051,361 + SO1948 0.67 -1.2 2,051,385 - SO1948 0.69 -2.9 2,051,385 - SO1948 0.69 -0.2 2,051,395 - SO1948 0.69 -0.3 2,051,457 + SO1948 0.74 -1.8 2,051,465 - SO1948 0.75 -1.2 2,051,468 + SO1948 0.75 -0.4 2,051,524 + SO1948 0.79 -1.1 2,051,719 - -0.7 2,051,719 - -1.3 2,051,965 + -1.0 2,051,996 + +1.8 2,052,004 - +0.3 2,052,015 + +0.3 2,052,054 - +0.5 2,052,054 - -1.8 2,052,087 - -0.1 2,052,087 - -1.1 2,052,087 - +1.4 2,052,087 - -2.6 2,052,087 - -1.4 2,052,122 + -0.2 2,052,130 - +0.3 2,052,205 + -1.4 2,052,213 + -2.4 2,052,213 + +1.9 2,052,213 + +0.4 2,052,213 + -0.1 2,052,213 + -1.3 2,052,213 - +0.4 2,052,221 - +1.6 2,052,233 - -0.3 2,052,354 + +1.2 2,052,377 - -0.8 2,052,425 + SO1949 0.11 +2.1 2,052,441 + SO1949 0.11 -0.2 2,052,451 + SO1949 0.12 +0.9 2,052,451 + SO1949 0.12 -0.3 2,052,459 - SO1949 0.12 +2.2 2,052,502 + SO1949 0.13 -2.1 2,052,509 - SO1949 0.13 -1.4 2,052,598 - SO1949 0.16 -1.8 2,052,628 + SO1949 0.16 +0.8 2,052,636 - SO1949 0.17 +0.5 2,052,716 + SO1949 0.19 +1.5
Or see this region's nucleotide sequence