Strain Fitness in Shewanella oneidensis MR-1 around SO1897

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpccB-1 and ivd are separated by 116 nucleotidesivd and SO1898 are separated by 144 nucleotidesSO1898 and SO1899 are separated by 260 nucleotides SO1896: pccB-1 - 3-methylcrotonyl CoA carboxylase, beta subunit (NCBI ptt file), at 1,993,425 to 1,995,032 pccB-1 SO1897: ivd - isovaleryl-CoA dehydrogenase (NCBI ptt file), at 1,995,149 to 1,996,318 ivd SO1898: SO1898 - transcriptional regulator, putative (NCBI ptt file), at 1,996,463 to 1,996,867 SO1898 SO1899: SO1899 - conserved hypothetical protein (NCBI ptt file), at 1,997,128 to 1,998,843 SO1899 Position (kb) 1995 1996 1997Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1994.261 kb on + strand, within pccB-1at 1994.269 kb on - strand, within pccB-1at 1994.269 kb on - strand, within pccB-1at 1994.469 kb on + strand, within pccB-1at 1994.472 kb on - strand, within pccB-1at 1994.477 kb on - strand, within pccB-1at 1994.503 kb on + strand, within pccB-1at 1994.927 kb on - strandat 1995.137 kb on + strandat 1995.142 kb on + strandat 1995.169 kb on - strandat 1995.204 kb on - strandat 1995.218 kb on + strandat 1995.218 kb on + strandat 1995.419 kb on + strand, within ivdat 1995.427 kb on - strand, within ivdat 1995.427 kb on - strand, within ivdat 1995.427 kb on - strand, within ivdat 1995.563 kb on - strand, within ivdat 1995.651 kb on + strand, within ivdat 1995.681 kb on - strand, within ivdat 1995.681 kb on - strand, within ivdat 1995.776 kb on - strand, within ivdat 1995.987 kb on + strand, within ivdat 1996.063 kb on - strand, within ivdat 1996.264 kb on + strandat 1996.299 kb on + strandat 1996.309 kb on - strandat 1996.534 kb on - strand, within SO1898at 1996.599 kb on + strand, within SO1898at 1996.599 kb on + strand, within SO1898at 1996.599 kb on + strand, within SO1898at 1996.599 kb on + strand, within SO1898at 1996.607 kb on - strand, within SO1898at 1996.622 kb on - strand, within SO1898at 1996.622 kb on - strand, within SO1898at 1996.668 kb on - strand, within SO1898at 1996.693 kb on + strand, within SO1898at 1996.693 kb on + strand, within SO1898at 1996.717 kb on + strand, within SO1898at 1996.723 kb on - strand, within SO1898at 1996.774 kb on - strand, within SO1898at 1996.873 kb on - strandat 1996.873 kb on - strandat 1997.057 kb on + strandat 1997.087 kb on - strandat 1997.087 kb on - strandat 1997.134 kb on - strandat 1997.134 kb on - strandat 1997.134 kb on - strandat 1997.138 kb on + strandat 1997.146 kb on - strandat 1997.146 kb on - strandat 1997.185 kb on + strandat 1997.193 kb on - strandat 1997.193 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,994,261 + pccB-1 SO1896 0.52 +0.6
1,994,269 - pccB-1 SO1896 0.52 +2.2
1,994,269 - pccB-1 SO1896 0.52 +1.2
1,994,469 + pccB-1 SO1896 0.65 +0.8
1,994,472 - pccB-1 SO1896 0.65 +1.3
1,994,477 - pccB-1 SO1896 0.65 -0.5
1,994,503 + pccB-1 SO1896 0.67 +0.7
1,994,927 - +1.1
1,995,137 + +2.1
1,995,142 + +2.9
1,995,169 - +0.5
1,995,204 - +2.1
1,995,218 + +1.6
1,995,218 + +1.4
1,995,419 + ivd SO1897 0.23 +3.9
1,995,427 - ivd SO1897 0.24 +2.7
1,995,427 - ivd SO1897 0.24 +3.1
1,995,427 - ivd SO1897 0.24 +2.4
1,995,563 - ivd SO1897 0.35 +2.6
1,995,651 + ivd SO1897 0.43 +3.5
1,995,681 - ivd SO1897 0.45 +2.7
1,995,681 - ivd SO1897 0.45 +3.4
1,995,776 - ivd SO1897 0.54 +3.2
1,995,987 + ivd SO1897 0.72 +2.3
1,996,063 - ivd SO1897 0.78 +2.1
1,996,264 + +4.2
1,996,299 + +0.2
1,996,309 - +2.4
1,996,534 - SO1898 0.18 -4.6
1,996,599 + SO1898 0.34 -3.1
1,996,599 + SO1898 0.34 -1.6
1,996,599 + SO1898 0.34 -3.8
1,996,599 + SO1898 0.34 -3.5
1,996,607 - SO1898 0.36 -3.7
1,996,622 - SO1898 0.39 -2.2
1,996,622 - SO1898 0.39 -1.5
1,996,668 - SO1898 0.51 -1.1
1,996,693 + SO1898 0.57 -2.6
1,996,693 + SO1898 0.57 -4.2
1,996,717 + SO1898 0.63 -3.2
1,996,723 - SO1898 0.64 -3.8
1,996,774 - SO1898 0.77 -3.3
1,996,873 - -0.2
1,996,873 - -0.8
1,997,057 + -0.6
1,997,087 - -0.7
1,997,087 - +0.3
1,997,134 - -1.3
1,997,134 - -1.1
1,997,134 - -2.3
1,997,138 + -2.6
1,997,146 - +0.5
1,997,146 - -2.1
1,997,185 + +0.9
1,997,193 - +1.4
1,997,193 - -3.1

Or see this region's nucleotide sequence