Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and pgsA are separated by 47 nucleotides pgsA and tRNA-Gly-5 are separated by 143 nucleotides tRNA-Gly-5 and tRNA-Cys-1 are separated by 21 nucleotides tRNA-Cys-1 and tRNA-Leu-2 are separated by 48 nucleotides tRNA-Leu-2 and tRNA-Leu-1 are separated by 132 nucleotides tRNA-Leu-1 and SO1863 are separated by 315 nucleotides
SO1861: uvrC - excinuclease ABC, C subunit (NCBI ptt file), at 1,952,556 to 1,954,385
uvrC
SO1862: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (NCBI ptt file), at 1,954,433 to 1,954,981
pgsA
SO_t033: tRNA-Gly-5 - tRNA-Gly (RefSeq), at 1,955,125 to 1,955,200
tRNA-Gly-5
SO_t034: tRNA-Cys-1 - tRNA-Cys (RefSeq), at 1,955,222 to 1,955,295
tRNA-Cys-1
SO_t035: tRNA-Leu-2 - tRNA-Leu (RefSeq), at 1,955,344 to 1,955,430
tRNA-Leu-2
SO_t036: tRNA-Leu-1 - tRNA-Leu (RefSeq), at 1,955,563 to 1,955,649
tRNA-Leu-1
SO1863: SO1863 - DNA-binding protein, HU family (NCBI ptt file), at 1,955,965 to 1,956,240
SO1863
Position (kb)
1954
1955 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1953.435 kb on + strand, within uvrC at 1953.435 kb on + strand, within uvrC at 1953.450 kb on - strand, within uvrC at 1953.543 kb on - strand, within uvrC at 1953.745 kb on + strand, within uvrC at 1953.778 kb on + strand, within uvrC at 1953.795 kb on + strand, within uvrC at 1953.864 kb on - strand, within uvrC at 1954.055 kb on + strand, within uvrC at 1954.063 kb on - strand, within uvrC at 1954.129 kb on + strand, within uvrC at 1955.397 kb on + strand, within tRNA-Leu-2 at 1955.448 kb on + strand at 1955.465 kb on + strand at 1955.465 kb on + strand at 1955.647 kb on + strand at 1955.778 kb on + strand at 1955.786 kb on - strand at 1955.786 kb on - strand at 1955.798 kb on + strand at 1955.822 kb on + strand at 1955.855 kb on - strand at 1955.879 kb on - strand at 1955.928 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,953,435 + uvrC SO1861 0.48 -0.3 1,953,435 + uvrC SO1861 0.48 -0.4 1,953,450 - uvrC SO1861 0.49 +0.9 1,953,543 - uvrC SO1861 0.54 -0.4 1,953,745 + uvrC SO1861 0.65 -1.1 1,953,778 + uvrC SO1861 0.67 +1.3 1,953,795 + uvrC SO1861 0.68 +2.0 1,953,864 - uvrC SO1861 0.71 +1.0 1,954,055 + uvrC SO1861 0.82 +0.2 1,954,063 - uvrC SO1861 0.82 -2.5 1,954,129 + uvrC SO1861 0.86 -1.7 1,955,397 + tRNA-Leu-2 SO_t035 0.61 -1.7 1,955,448 + -1.7 1,955,465 + +1.7 1,955,465 + -0.2 1,955,647 + +1.8 1,955,778 + +1.6 1,955,786 - +1.0 1,955,786 - +1.8 1,955,798 + +0.7 1,955,822 + -0.9 1,955,855 - +1.2 1,955,879 - -1.3 1,955,928 + +0.9
Or see this region's nucleotide sequence