Strain Fitness in Shewanella oneidensis MR-1 around SO1862

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuvrC and pgsA are separated by 47 nucleotidespgsA and tRNA-Gly-5 are separated by 143 nucleotidestRNA-Gly-5 and tRNA-Cys-1 are separated by 21 nucleotidestRNA-Cys-1 and tRNA-Leu-2 are separated by 48 nucleotidestRNA-Leu-2 and tRNA-Leu-1 are separated by 132 nucleotidestRNA-Leu-1 and SO1863 are separated by 315 nucleotides SO1861: uvrC - excinuclease ABC, C subunit (NCBI ptt file), at 1,952,556 to 1,954,385 uvrC SO1862: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (NCBI ptt file), at 1,954,433 to 1,954,981 pgsA SO_t033: tRNA-Gly-5 - tRNA-Gly (RefSeq), at 1,955,125 to 1,955,200 tRNA-Gly-5 SO_t034: tRNA-Cys-1 - tRNA-Cys (RefSeq), at 1,955,222 to 1,955,295 tRNA-Cys-1 SO_t035: tRNA-Leu-2 - tRNA-Leu (RefSeq), at 1,955,344 to 1,955,430 tRNA-Leu-2 SO_t036: tRNA-Leu-1 - tRNA-Leu (RefSeq), at 1,955,563 to 1,955,649 tRNA-Leu-1 SO1863: SO1863 - DNA-binding protein, HU family (NCBI ptt file), at 1,955,965 to 1,956,240 SO1863 Position (kb) 1954 1955Strain fitness (log2 ratio) -2 -1 0 1 2at 1953.435 kb on + strand, within uvrCat 1953.435 kb on + strand, within uvrCat 1953.450 kb on - strand, within uvrCat 1953.543 kb on - strand, within uvrCat 1953.745 kb on + strand, within uvrCat 1953.778 kb on + strand, within uvrCat 1953.795 kb on + strand, within uvrCat 1953.864 kb on - strand, within uvrCat 1954.055 kb on + strand, within uvrCat 1954.063 kb on - strand, within uvrCat 1954.129 kb on + strand, within uvrCat 1955.397 kb on + strand, within tRNA-Leu-2at 1955.448 kb on + strandat 1955.465 kb on + strandat 1955.465 kb on + strandat 1955.647 kb on + strandat 1955.778 kb on + strandat 1955.786 kb on - strandat 1955.786 kb on - strandat 1955.798 kb on + strandat 1955.822 kb on + strandat 1955.855 kb on - strandat 1955.879 kb on - strandat 1955.928 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,953,435 + uvrC SO1861 0.48 -0.3
1,953,435 + uvrC SO1861 0.48 -0.4
1,953,450 - uvrC SO1861 0.49 +0.9
1,953,543 - uvrC SO1861 0.54 -0.4
1,953,745 + uvrC SO1861 0.65 -1.1
1,953,778 + uvrC SO1861 0.67 +1.3
1,953,795 + uvrC SO1861 0.68 +2.0
1,953,864 - uvrC SO1861 0.71 +1.0
1,954,055 + uvrC SO1861 0.82 +0.2
1,954,063 - uvrC SO1861 0.82 -2.5
1,954,129 + uvrC SO1861 0.86 -1.7
1,955,397 + tRNA-Leu-2 SO_t035 0.61 -1.7
1,955,448 + -1.7
1,955,465 + +1.7
1,955,465 + -0.2
1,955,647 + +1.8
1,955,778 + +1.6
1,955,786 - +1.0
1,955,786 - +1.8
1,955,798 + +0.7
1,955,822 + -0.9
1,955,855 - +1.2
1,955,879 - -1.3
1,955,928 + +0.9

Or see this region's nucleotide sequence