Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1748 and SO1750 are separated by 527 nucleotides SO1750 and SO1751 are separated by 12 nucleotides
SO1748: SO1748 - hypothetical protein (NCBI ptt file), at 1,828,761 to 1,830,290
SO1748
SO1750: SO1750 - ABC transporter, ATP-binding protein (NCBI ptt file), at 1,830,818 to 1,831,696
SO1750
SO1751: SO1751 - membrane protein, putative (NCBI ptt file), at 1,831,709 to 1,835,266
SO1751
Position (kb)
1830
1831
1832 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1829.962 kb on - strand, within SO1748 at 1830.091 kb on + strand, within SO1748 at 1830.190 kb on + strand at 1830.198 kb on - strand at 1830.198 kb on - strand at 1830.203 kb on - strand at 1830.204 kb on - strand at 1830.204 kb on - strand at 1830.247 kb on + strand at 1830.276 kb on + strand at 1830.279 kb on - strand at 1830.740 kb on - strand at 1830.752 kb on + strand at 1830.752 kb on + strand at 1830.752 kb on + strand at 1830.793 kb on - strand at 1830.866 kb on - strand at 1830.918 kb on + strand, within SO1750 at 1831.081 kb on + strand, within SO1750 at 1831.089 kb on - strand, within SO1750 at 1831.089 kb on - strand, within SO1750 at 1831.125 kb on + strand, within SO1750 at 1831.235 kb on - strand, within SO1750 at 1831.250 kb on + strand, within SO1750 at 1831.258 kb on - strand, within SO1750 at 1831.311 kb on + strand, within SO1750 at 1831.311 kb on + strand, within SO1750 at 1831.311 kb on + strand, within SO1750 at 1831.311 kb on + strand, within SO1750 at 1831.319 kb on - strand, within SO1750 at 1831.329 kb on - strand, within SO1750 at 1831.401 kb on + strand, within SO1750 at 1831.401 kb on + strand, within SO1750 at 1831.409 kb on - strand, within SO1750 at 1831.409 kb on - strand, within SO1750 at 1831.409 kb on - strand, within SO1750 at 1831.519 kb on + strand, within SO1750 at 1831.534 kb on - strand, within SO1750 at 1831.601 kb on + strand, within SO1750 at 1831.620 kb on + strand at 1831.698 kb on - strand at 1831.698 kb on - strand at 1831.858 kb on - strand at 1831.981 kb on + strand at 1832.313 kb on + strand, within SO1751 at 1832.326 kb on + strand, within SO1751 at 1832.342 kb on - strand, within SO1751 at 1832.614 kb on + strand, within SO1751 at 1832.636 kb on + strand, within SO1751 at 1832.694 kb on + strand, within SO1751
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,829,962 - SO1748 0.78 +0.1 1,830,091 + SO1748 0.87 -0.6 1,830,190 + +1.8 1,830,198 - +0.7 1,830,198 - +0.1 1,830,203 - +3.4 1,830,204 - +0.4 1,830,204 - +0.2 1,830,247 + +1.2 1,830,276 + -1.6 1,830,279 - -1.4 1,830,740 - -1.9 1,830,752 + +0.2 1,830,752 + -0.6 1,830,752 + -0.1 1,830,793 - +1.1 1,830,866 - +0.4 1,830,918 + SO1750 0.11 -0.1 1,831,081 + SO1750 0.30 -1.7 1,831,089 - SO1750 0.31 -0.6 1,831,089 - SO1750 0.31 -0.7 1,831,125 + SO1750 0.35 -2.5 1,831,235 - SO1750 0.47 +1.4 1,831,250 + SO1750 0.49 -0.6 1,831,258 - SO1750 0.50 -2.2 1,831,311 + SO1750 0.56 -2.3 1,831,311 + SO1750 0.56 -1.2 1,831,311 + SO1750 0.56 -2.2 1,831,311 + SO1750 0.56 -0.6 1,831,319 - SO1750 0.57 -2.8 1,831,329 - SO1750 0.58 +0.5 1,831,401 + SO1750 0.66 -0.2 1,831,401 + SO1750 0.66 -3.2 1,831,409 - SO1750 0.67 -1.9 1,831,409 - SO1750 0.67 -1.8 1,831,409 - SO1750 0.67 -2.2 1,831,519 + SO1750 0.80 -1.2 1,831,534 - SO1750 0.81 +0.2 1,831,601 + SO1750 0.89 -1.3 1,831,620 + -1.1 1,831,698 - -3.6 1,831,698 - +0.4 1,831,858 - -0.1 1,831,981 + +0.4 1,832,313 + SO1751 0.17 -2.0 1,832,326 + SO1751 0.17 -1.2 1,832,342 - SO1751 0.18 +0.4 1,832,614 + SO1751 0.25 -1.9 1,832,636 + SO1751 0.26 +0.0 1,832,694 + SO1751 0.28 -0.9
Or see this region's nucleotide sequence