Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1727 and SO1728 are separated by 235 nucleotides SO1728 and SO1732 are separated by 510 nucleotides SO1732 and SO1733 are separated by 85 nucleotides SO1733 and SO1734 are separated by 66 nucleotides
SO1727: SO1727 - hypothetical protein (NCBI ptt file), at 1,814,473 to 1,814,985
SO1727
SO1728: SO1728 - hypothetical protein (NCBI ptt file), at 1,815,221 to 1,815,463
SO1728
SO1732: SO1732 - cold shock domain family protein (NCBI ptt file), at 1,815,974 to 1,816,648
SO1732
SO1733: SO1733 - hypothetical protein (NCBI ptt file), at 1,816,734 to 1,817,333
SO1733
SO1734: SO1734 - glyoxalase family protein (NCBI ptt file), at 1,817,400 to 1,817,795
SO1734
Position (kb)
1815
1816
1817 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1815.311 kb on + strand, within SO1728 at 1815.321 kb on - strand, within SO1728 at 1815.353 kb on - strand, within SO1728 at 1815.463 kb on + strand at 1815.581 kb on - strand at 1815.583 kb on + strand at 1815.583 kb on + strand at 1815.583 kb on + strand at 1815.631 kb on - strand at 1815.833 kb on - strand at 1815.844 kb on + strand at 1815.844 kb on + strand at 1815.850 kb on - strand at 1815.850 kb on - strand at 1815.850 kb on - strand at 1815.850 kb on - strand at 1815.895 kb on + strand at 1815.962 kb on + strand at 1816.041 kb on - strand at 1816.041 kb on - strand at 1816.071 kb on - strand, within SO1732 at 1816.183 kb on - strand, within SO1732 at 1816.202 kb on + strand, within SO1732 at 1816.225 kb on - strand, within SO1732 at 1816.312 kb on + strand, within SO1732 at 1816.320 kb on - strand, within SO1732 at 1816.320 kb on - strand, within SO1732 at 1816.406 kb on - strand, within SO1732 at 1816.478 kb on + strand, within SO1732 at 1816.478 kb on + strand, within SO1732 at 1816.480 kb on + strand, within SO1732 at 1816.480 kb on + strand, within SO1732 at 1816.486 kb on - strand, within SO1732 at 1816.486 kb on - strand, within SO1732 at 1816.522 kb on + strand, within SO1732 at 1816.525 kb on - strand, within SO1732 at 1816.920 kb on - strand, within SO1733 at 1816.920 kb on - strand, within SO1733 at 1817.061 kb on + strand, within SO1733 at 1817.080 kb on - strand, within SO1733 at 1817.087 kb on + strand, within SO1733 at 1817.087 kb on + strand, within SO1733 at 1817.091 kb on - strand, within SO1733 at 1817.143 kb on + strand, within SO1733 at 1817.190 kb on + strand, within SO1733 at 1817.432 kb on + strand at 1817.432 kb on + strand at 1817.440 kb on - strand, within SO1734 at 1817.460 kb on + strand, within SO1734 at 1817.470 kb on + strand, within SO1734 at 1817.470 kb on + strand, within SO1734 at 1817.484 kb on + strand, within SO1734 at 1817.484 kb on + strand, within SO1734 at 1817.504 kb on - strand, within SO1734 at 1817.541 kb on - strand, within SO1734 at 1817.614 kb on + strand, within SO1734
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,815,311 + SO1728 0.37 +1.2 1,815,321 - SO1728 0.41 -1.2 1,815,353 - SO1728 0.54 -0.7 1,815,463 + +1.3 1,815,581 - +1.8 1,815,583 + -1.2 1,815,583 + +0.4 1,815,583 + +0.7 1,815,631 - -1.6 1,815,833 - +0.5 1,815,844 + +1.4 1,815,844 + +1.9 1,815,850 - +1.6 1,815,850 - +1.2 1,815,850 - +0.1 1,815,850 - +0.2 1,815,895 + +0.5 1,815,962 + -0.9 1,816,041 - -0.3 1,816,041 - -0.9 1,816,071 - SO1732 0.14 +0.6 1,816,183 - SO1732 0.31 -0.8 1,816,202 + SO1732 0.34 -0.4 1,816,225 - SO1732 0.37 +0.7 1,816,312 + SO1732 0.50 -1.1 1,816,320 - SO1732 0.51 -0.6 1,816,320 - SO1732 0.51 -1.9 1,816,406 - SO1732 0.64 +0.5 1,816,478 + SO1732 0.75 -2.0 1,816,478 + SO1732 0.75 +1.3 1,816,480 + SO1732 0.75 -0.5 1,816,480 + SO1732 0.75 +0.9 1,816,486 - SO1732 0.76 -1.0 1,816,486 - SO1732 0.76 +0.1 1,816,522 + SO1732 0.81 +0.4 1,816,525 - SO1732 0.82 +0.2 1,816,920 - SO1733 0.31 -1.1 1,816,920 - SO1733 0.31 -2.4 1,817,061 + SO1733 0.55 +0.0 1,817,080 - SO1733 0.58 -0.4 1,817,087 + SO1733 0.59 +1.8 1,817,087 + SO1733 0.59 -2.2 1,817,091 - SO1733 0.59 +0.2 1,817,143 + SO1733 0.68 +0.4 1,817,190 + SO1733 0.76 +5.0 1,817,432 + +0.6 1,817,432 + +0.6 1,817,440 - SO1734 0.10 -0.5 1,817,460 + SO1734 0.15 +0.4 1,817,470 + SO1734 0.18 +1.3 1,817,470 + SO1734 0.18 -2.0 1,817,484 + SO1734 0.21 -1.3 1,817,484 + SO1734 0.21 -1.6 1,817,504 - SO1734 0.26 +0.4 1,817,541 - SO1734 0.36 -0.6 1,817,614 + SO1734 0.54 -1.0
Or see this region's nucleotide sequence