Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1681 and mmsB are separated by 83 nucleotides mmsB and SO1683 are separated by 24 nucleotides SO1683 and SO1685 are separated by 207 nucleotides
SO1681: SO1681 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,768,155 to 1,769,306
SO1681
SO1682: mmsB - 3-hydroxyisobutyrate dehydrogenase (NCBI ptt file), at 1,769,390 to 1,770,292
mmsB
SO1683: SO1683 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase IvdG (EC:1.1.1.178) (from data) , at 1,770,317 to 1,771,075
SO1683
SO1685: SO1685 - hypothetical protein (NCBI ptt file), at 1,771,283 to 1,771,534
SO1685
Position (kb)
1769
1770
1771 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.650 kb on + strand, within SO1681 at 1768.717 kb on + strand, within SO1681 at 1768.752 kb on - strand, within SO1681 at 1768.880 kb on - strand, within SO1681 at 1768.921 kb on + strand, within SO1681 at 1769.040 kb on + strand, within SO1681 at 1769.040 kb on + strand, within SO1681 at 1769.080 kb on - strand, within SO1681 at 1769.090 kb on + strand, within SO1681 at 1769.240 kb on - strand at 1769.242 kb on + strand at 1769.250 kb on - strand at 1769.276 kb on - strand at 1769.309 kb on - strand at 1769.340 kb on - strand at 1769.375 kb on - strand at 1769.392 kb on + strand at 1769.402 kb on - strand at 1769.438 kb on - strand at 1769.707 kb on + strand, within mmsB at 1769.726 kb on + strand, within mmsB at 1769.783 kb on + strand, within mmsB at 1769.835 kb on - strand, within mmsB at 1769.844 kb on - strand, within mmsB at 1769.895 kb on - strand, within mmsB at 1769.960 kb on - strand, within mmsB at 1769.981 kb on - strand, within mmsB at 1770.060 kb on - strand, within mmsB at 1770.106 kb on - strand, within mmsB at 1770.362 kb on + strand at 1770.441 kb on - strand, within SO1683 at 1770.527 kb on + strand, within SO1683 at 1770.663 kb on - strand, within SO1683 at 1770.680 kb on - strand, within SO1683 at 1771.254 kb on + strand at 1771.254 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,768,526 + SO1681 0.32 -3.5 1,768,526 + SO1681 0.32 -3.8 1,768,526 + SO1681 0.32 -1.9 1,768,526 + SO1681 0.32 -3.8 1,768,650 + SO1681 0.43 -4.6 1,768,717 + SO1681 0.49 -4.1 1,768,752 - SO1681 0.52 -2.4 1,768,880 - SO1681 0.63 -4.2 1,768,921 + SO1681 0.66 -2.9 1,769,040 + SO1681 0.77 -1.2 1,769,040 + SO1681 0.77 -4.6 1,769,080 - SO1681 0.80 -3.7 1,769,090 + SO1681 0.81 -1.9 1,769,240 - -3.8 1,769,242 + -1.9 1,769,250 - -3.3 1,769,276 - -2.6 1,769,309 - -1.2 1,769,340 - -2.4 1,769,375 - -3.5 1,769,392 + -5.1 1,769,402 - -0.9 1,769,438 - -3.7 1,769,707 + mmsB SO1682 0.35 -2.6 1,769,726 + mmsB SO1682 0.37 -5.4 1,769,783 + mmsB SO1682 0.44 -3.1 1,769,835 - mmsB SO1682 0.49 -2.5 1,769,844 - mmsB SO1682 0.50 -5.1 1,769,895 - mmsB SO1682 0.56 -4.0 1,769,960 - mmsB SO1682 0.63 -2.4 1,769,981 - mmsB SO1682 0.65 -4.5 1,770,060 - mmsB SO1682 0.74 -3.4 1,770,106 - mmsB SO1682 0.79 -4.5 1,770,362 + -0.9 1,770,441 - SO1683 0.16 +0.4 1,770,527 + SO1683 0.28 -1.9 1,770,663 - SO1683 0.46 -4.9 1,770,680 - SO1683 0.48 -3.9 1,771,254 + +0.1 1,771,254 + +2.4
Or see this region's nucleotide sequence