Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metA and atoB are separated by 430 nucleotides atoB and mmsA are separated by 136 nucleotides
SO1676: metA - homoserine O-succinyltransferase (NCBI ptt file), at 1,761,811 to 1,762,752
metA
SO1677: atoB - 3-ketoacyl-CoA thiolase [isoleucine degradation] (EC 2.3.1.16) (from data) , at 1,763,183 to 1,764,373
atoB
SO1678: mmsA - methylmalonate-semialdehyde dehydrogenase (NCBI ptt file), at 1,764,510 to 1,766,009
mmsA
Position (kb)
1763
1764
1765 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1762.355 kb on + strand, within metA at 1762.406 kb on - strand, within metA at 1762.445 kb on - strand, within metA at 1762.614 kb on + strand, within metA at 1762.679 kb on + strand at 1762.680 kb on + strand at 1762.801 kb on + strand at 1762.825 kb on + strand at 1762.941 kb on + strand at 1763.015 kb on - strand at 1763.187 kb on + strand at 1763.187 kb on - strand at 1763.284 kb on - strand at 1763.459 kb on - strand, within atoB at 1763.517 kb on + strand, within atoB at 1763.599 kb on - strand, within atoB at 1763.599 kb on - strand, within atoB at 1763.611 kb on - strand, within atoB at 1763.623 kb on - strand, within atoB at 1763.763 kb on - strand, within atoB at 1763.767 kb on + strand, within atoB at 1763.814 kb on + strand, within atoB at 1763.817 kb on - strand, within atoB at 1763.854 kb on + strand, within atoB at 1764.074 kb on - strand, within atoB at 1764.131 kb on - strand, within atoB at 1764.149 kb on + strand, within atoB at 1764.275 kb on + strand at 1764.290 kb on + strand at 1764.298 kb on - strand at 1764.315 kb on + strand at 1764.315 kb on + strand at 1764.323 kb on - strand at 1764.323 kb on - strand at 1764.330 kb on + strand at 1764.361 kb on - strand at 1764.424 kb on - strand at 1764.429 kb on - strand at 1764.479 kb on + strand at 1764.479 kb on + strand at 1764.492 kb on - strand at 1764.656 kb on - strand at 1764.793 kb on + strand, within mmsA at 1765.069 kb on - strand, within mmsA at 1765.069 kb on - strand, within mmsA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,762,355 + metA SO1676 0.58 -4.2 1,762,406 - metA SO1676 0.63 -4.3 1,762,445 - metA SO1676 0.67 -4.9 1,762,614 + metA SO1676 0.85 -3.3 1,762,679 + -3.3 1,762,680 + -2.8 1,762,801 + -1.2 1,762,825 + +0.2 1,762,941 + +3.9 1,763,015 - +0.2 1,763,187 + -3.8 1,763,187 - -2.2 1,763,284 - -2.2 1,763,459 - atoB SO1677 0.23 -4.4 1,763,517 + atoB SO1677 0.28 -2.8 1,763,599 - atoB SO1677 0.35 -3.2 1,763,599 - atoB SO1677 0.35 -3.2 1,763,611 - atoB SO1677 0.36 -2.9 1,763,623 - atoB SO1677 0.37 -2.4 1,763,763 - atoB SO1677 0.49 -3.3 1,763,767 + atoB SO1677 0.49 -2.2 1,763,814 + atoB SO1677 0.53 -3.5 1,763,817 - atoB SO1677 0.53 -3.0 1,763,854 + atoB SO1677 0.56 -3.2 1,764,074 - atoB SO1677 0.75 -3.4 1,764,131 - atoB SO1677 0.80 -5.0 1,764,149 + atoB SO1677 0.81 -3.2 1,764,275 + -2.8 1,764,290 + -2.9 1,764,298 - -2.2 1,764,315 + -5.7 1,764,315 + -3.4 1,764,323 - -3.5 1,764,323 - -3.3 1,764,330 + -5.0 1,764,361 - -1.9 1,764,424 - -4.1 1,764,429 - -2.8 1,764,479 + -2.2 1,764,479 + -3.7 1,764,492 - -4.5 1,764,656 - -4.8 1,764,793 + mmsA SO1678 0.19 -4.2 1,765,069 - mmsA SO1678 0.37 -4.4 1,765,069 - mmsA SO1678 0.37 -3.7
Or see this region's nucleotide sequence