Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1656 and SO1657 are separated by 96 nucleotides SO1657 and SO1658 are separated by 4 nucleotides SO1658 and SO1659 are separated by 52 nucleotides
SO1656: SO1656 - ROK family protein (NCBI ptt file), at 1,743,565 to 1,744,485
SO1656
SO1657: SO1657 - conserved hypothetical protein (NCBI ptt file), at 1,744,582 to 1,745,316
SO1657
SO1658: SO1658 - hypothetical protein (NCBI ptt file), at 1,745,321 to 1,746,052
SO1658
SO1659: SO1659 - decaheme cytochrome c (NCBI ptt file), at 1,746,105 to 1,748,381
SO1659
Position (kb)
1745
1746
1747 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1744.327 kb on - strand, within SO1656 at 1744.349 kb on - strand, within SO1656 at 1744.463 kb on - strand at 1744.473 kb on - strand at 1744.557 kb on + strand at 1744.557 kb on + strand at 1744.623 kb on - strand at 1744.790 kb on - strand, within SO1657 at 1744.794 kb on + strand, within SO1657 at 1744.802 kb on - strand, within SO1657 at 1744.925 kb on - strand, within SO1657 at 1745.006 kb on - strand, within SO1657 at 1745.024 kb on - strand, within SO1657 at 1745.359 kb on + strand at 1745.378 kb on + strand at 1745.378 kb on + strand at 1745.378 kb on + strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.464 kb on - strand, within SO1658 at 1745.480 kb on - strand, within SO1658 at 1745.516 kb on - strand, within SO1658 at 1745.634 kb on - strand, within SO1658 at 1745.651 kb on - strand, within SO1658 at 1745.752 kb on + strand, within SO1658 at 1745.752 kb on + strand, within SO1658 at 1745.777 kb on + strand, within SO1658 at 1745.880 kb on - strand, within SO1658 at 1745.928 kb on + strand, within SO1658 at 1745.936 kb on - strand, within SO1658 at 1745.990 kb on - strand at 1745.991 kb on - strand at 1746.069 kb on - strand at 1746.074 kb on - strand at 1746.074 kb on - strand at 1746.110 kb on - strand at 1746.368 kb on + strand, within SO1659 at 1746.376 kb on - strand, within SO1659 at 1746.376 kb on - strand, within SO1659 at 1746.378 kb on + strand, within SO1659 at 1746.429 kb on - strand, within SO1659 at 1746.429 kb on - strand, within SO1659 at 1746.429 kb on - strand, within SO1659 at 1746.496 kb on + strand, within SO1659 at 1746.527 kb on + strand, within SO1659 at 1746.535 kb on - strand, within SO1659 at 1746.535 kb on - strand, within SO1659 at 1746.535 kb on - strand, within SO1659 at 1746.535 kb on - strand, within SO1659 at 1746.535 kb on - strand, within SO1659 at 1746.612 kb on + strand, within SO1659 at 1746.621 kb on - strand, within SO1659 at 1746.621 kb on - strand, within SO1659 at 1746.663 kb on + strand, within SO1659 at 1746.671 kb on - strand, within SO1659 at 1746.760 kb on - strand, within SO1659 at 1746.859 kb on + strand, within SO1659 at 1746.932 kb on - strand, within SO1659 at 1747.006 kb on - strand, within SO1659 at 1747.019 kb on + strand, within SO1659
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,744,327 - SO1656 0.83 +1.2 1,744,349 - SO1656 0.85 +2.1 1,744,463 - +2.0 1,744,473 - -1.8 1,744,557 + -0.1 1,744,557 + +0.3 1,744,623 - -3.6 1,744,790 - SO1657 0.28 -0.6 1,744,794 + SO1657 0.29 -0.0 1,744,802 - SO1657 0.30 -1.1 1,744,925 - SO1657 0.47 +0.5 1,745,006 - SO1657 0.58 -1.9 1,745,024 - SO1657 0.60 -1.9 1,745,359 + -1.4 1,745,378 + +0.6 1,745,378 + +0.2 1,745,378 + +2.1 1,745,386 - +0.1 1,745,386 - -2.9 1,745,386 - +1.0 1,745,386 - +0.4 1,745,386 - +1.9 1,745,464 - SO1658 0.20 -0.1 1,745,480 - SO1658 0.22 +0.1 1,745,516 - SO1658 0.27 +1.6 1,745,634 - SO1658 0.43 +1.4 1,745,651 - SO1658 0.45 -0.9 1,745,752 + SO1658 0.59 +0.3 1,745,752 + SO1658 0.59 -1.4 1,745,777 + SO1658 0.62 +2.5 1,745,880 - SO1658 0.76 -0.3 1,745,928 + SO1658 0.83 -0.5 1,745,936 - SO1658 0.84 +0.4 1,745,990 - -0.8 1,745,991 - -0.0 1,746,069 - -1.9 1,746,074 - +1.4 1,746,074 - +0.0 1,746,110 - -0.2 1,746,368 + SO1659 0.12 +0.4 1,746,376 - SO1659 0.12 -1.9 1,746,376 - SO1659 0.12 +2.5 1,746,378 + SO1659 0.12 +0.9 1,746,429 - SO1659 0.14 +0.4 1,746,429 - SO1659 0.14 +1.2 1,746,429 - SO1659 0.14 +1.5 1,746,496 + SO1659 0.17 +0.6 1,746,527 + SO1659 0.19 +1.7 1,746,535 - SO1659 0.19 -0.1 1,746,535 - SO1659 0.19 +1.1 1,746,535 - SO1659 0.19 -1.1 1,746,535 - SO1659 0.19 +1.2 1,746,535 - SO1659 0.19 +0.6 1,746,612 + SO1659 0.22 +0.1 1,746,621 - SO1659 0.23 -1.0 1,746,621 - SO1659 0.23 +1.5 1,746,663 + SO1659 0.25 -1.1 1,746,671 - SO1659 0.25 -0.0 1,746,760 - SO1659 0.29 -3.2 1,746,859 + SO1659 0.33 +0.9 1,746,932 - SO1659 0.36 +0.3 1,747,006 - SO1659 0.40 -1.5 1,747,019 + SO1659 0.40 +0.2
Or see this region's nucleotide sequence