Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lgt and thyA overlap by 1 nucleotides thyA and nhaA are separated by 118 nucleotides nhaA and SO1337 are separated by 2 nucleotides SO1337 and nhaR are separated by 94 nucleotides
SO1334: lgt - prolipoprotein diacylglyceryl transferase (NCBI ptt file), at 1,392,260 to 1,393,066
lgt
SO1335: thyA - thymidylate synthase (NCBI ptt file), at 1,393,066 to 1,393,860
thyA
SO1336: nhaA - Na+/H+ antiporter (NCBI ptt file), at 1,393,979 to 1,395,148
nhaA
SO1337: SO1337 - hypothetical protein (NCBI ptt file), at 1,395,151 to 1,395,537
SO1337
SO1338: nhaR - transcriptional activator protein NhaR (NCBI ptt file), at 1,395,632 to 1,396,540
nhaR
Position (kb)
1393
1394
1395
1396 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1394.042 kb on + strand at 1394.048 kb on + strand at 1394.065 kb on + strand at 1394.080 kb on + strand at 1394.151 kb on - strand, within nhaA at 1394.208 kb on - strand, within nhaA at 1394.212 kb on + strand, within nhaA at 1394.212 kb on + strand, within nhaA at 1394.260 kb on - strand, within nhaA at 1394.410 kb on + strand, within nhaA at 1394.503 kb on - strand, within nhaA at 1394.564 kb on + strand, within nhaA at 1394.589 kb on + strand, within nhaA at 1394.597 kb on - strand, within nhaA at 1394.613 kb on + strand, within nhaA at 1394.621 kb on - strand, within nhaA at 1394.643 kb on - strand, within nhaA at 1394.683 kb on + strand, within nhaA at 1394.747 kb on + strand, within nhaA at 1394.782 kb on - strand, within nhaA at 1395.047 kb on - strand at 1395.062 kb on + strand at 1395.070 kb on - strand at 1395.194 kb on - strand, within SO1337 at 1395.266 kb on + strand, within SO1337 at 1395.274 kb on - strand, within SO1337 at 1395.421 kb on + strand, within SO1337 at 1395.490 kb on + strand, within SO1337 at 1395.738 kb on - strand, within nhaR at 1395.754 kb on + strand, within nhaR at 1395.872 kb on - strand, within nhaR at 1395.872 kb on - strand, within nhaR at 1396.016 kb on - strand, within nhaR at 1396.036 kb on - strand, within nhaR
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,394,042 + -1.5 1,394,048 + -0.5 1,394,065 + +0.7 1,394,080 + -0.3 1,394,151 - nhaA SO1336 0.15 -0.7 1,394,208 - nhaA SO1336 0.20 +0.9 1,394,212 + nhaA SO1336 0.20 +2.3 1,394,212 + nhaA SO1336 0.20 +1.2 1,394,260 - nhaA SO1336 0.24 -0.2 1,394,410 + nhaA SO1336 0.37 +0.6 1,394,503 - nhaA SO1336 0.45 +0.9 1,394,564 + nhaA SO1336 0.50 -2.6 1,394,589 + nhaA SO1336 0.52 -2.5 1,394,597 - nhaA SO1336 0.53 -0.2 1,394,613 + nhaA SO1336 0.54 +1.9 1,394,621 - nhaA SO1336 0.55 +0.1 1,394,643 - nhaA SO1336 0.57 -0.3 1,394,683 + nhaA SO1336 0.60 +0.6 1,394,747 + nhaA SO1336 0.66 +1.7 1,394,782 - nhaA SO1336 0.69 +2.6 1,395,047 - +0.4 1,395,062 + -0.3 1,395,070 - +4.8 1,395,194 - SO1337 0.11 +1.2 1,395,266 + SO1337 0.30 +0.3 1,395,274 - SO1337 0.32 +1.0 1,395,421 + SO1337 0.70 -0.2 1,395,490 + SO1337 0.88 -2.3 1,395,738 - nhaR SO1338 0.12 +0.6 1,395,754 + nhaR SO1338 0.13 -1.2 1,395,872 - nhaR SO1338 0.26 -0.8 1,395,872 - nhaR SO1338 0.26 -0.6 1,396,016 - nhaR SO1338 0.42 -1.7 1,396,036 - nhaR SO1338 0.44 +3.0
Or see this region's nucleotide sequence