Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1261 and SO1262 are separated by 93 nucleotides SO1262 and SO1263 are separated by 76 nucleotides SO1263 and SO1264 are separated by 175 nucleotides
SO1261: SO1261 - mercaptopyruvate sulfurtransferase (NCBI ptt file), at 1,310,077 to 1,310,934
SO1261
SO1262: SO1262 - hypothetical protein (NCBI ptt file), at 1,311,028 to 1,311,540
SO1262
SO1263: SO1263 - hypothetical protein (NCBI ptt file), at 1,311,617 to 1,311,712
SO1263
SO1264: SO1264 - hypothetical oxidoreductase (NCBI ptt file), at 1,311,888 to 1,313,195
SO1264
Position (kb)
1311
1312 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1310.028 kb on - strand at 1310.062 kb on + strand at 1310.062 kb on + strand at 1310.094 kb on - strand at 1310.104 kb on - strand at 1310.105 kb on + strand at 1310.121 kb on - strand at 1310.145 kb on - strand at 1310.159 kb on - strand at 1310.222 kb on + strand, within SO1261 at 1310.260 kb on + strand, within SO1261 at 1310.268 kb on - strand, within SO1261 at 1310.313 kb on + strand, within SO1261 at 1310.429 kb on + strand, within SO1261 at 1310.440 kb on + strand, within SO1261 at 1310.447 kb on + strand, within SO1261 at 1310.452 kb on + strand, within SO1261 at 1310.452 kb on + strand, within SO1261 at 1310.460 kb on - strand, within SO1261 at 1310.460 kb on - strand, within SO1261 at 1310.460 kb on - strand, within SO1261 at 1310.460 kb on - strand, within SO1261 at 1310.462 kb on + strand, within SO1261 at 1310.462 kb on + strand, within SO1261 at 1310.585 kb on + strand, within SO1261 at 1310.585 kb on + strand, within SO1261 at 1310.716 kb on + strand, within SO1261 at 1310.872 kb on + strand at 1310.896 kb on + strand at 1310.985 kb on - strand at 1310.992 kb on - strand at 1311.129 kb on + strand, within SO1262 at 1311.129 kb on + strand, within SO1262 at 1311.137 kb on - strand, within SO1262 at 1311.137 kb on - strand, within SO1262 at 1311.137 kb on - strand, within SO1262 at 1311.154 kb on + strand, within SO1262 at 1311.154 kb on + strand, within SO1262 at 1311.154 kb on + strand, within SO1262 at 1311.162 kb on - strand, within SO1262 at 1311.184 kb on - strand, within SO1262 at 1311.186 kb on + strand, within SO1262 at 1311.470 kb on + strand, within SO1262 at 1311.485 kb on + strand, within SO1262 at 1311.497 kb on + strand at 1311.552 kb on - strand at 1311.802 kb on + strand at 1311.812 kb on + strand at 1311.812 kb on + strand at 1311.812 kb on + strand at 1311.899 kb on + strand at 1311.940 kb on - strand at 1312.042 kb on + strand, within SO1264 at 1312.080 kb on - strand, within SO1264 at 1312.080 kb on - strand, within SO1264 at 1312.110 kb on - strand, within SO1264 at 1312.163 kb on + strand, within SO1264 at 1312.183 kb on + strand, within SO1264 at 1312.183 kb on + strand, within SO1264 at 1312.191 kb on - strand, within SO1264 at 1312.191 kb on - strand, within SO1264 at 1312.191 kb on - strand, within SO1264 at 1312.226 kb on - strand, within SO1264 at 1312.228 kb on + strand, within SO1264 at 1312.261 kb on + strand, within SO1264 at 1312.460 kb on + strand, within SO1264
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,310,028 - +0.1 1,310,062 + -2.4 1,310,062 + -0.3 1,310,094 - -1.4 1,310,104 - +1.7 1,310,105 + -0.7 1,310,121 - +0.0 1,310,145 - -0.6 1,310,159 - +0.4 1,310,222 + SO1261 0.17 -0.7 1,310,260 + SO1261 0.21 -0.3 1,310,268 - SO1261 0.22 +1.5 1,310,313 + SO1261 0.28 +1.1 1,310,429 + SO1261 0.41 -1.2 1,310,440 + SO1261 0.42 +0.0 1,310,447 + SO1261 0.43 -0.6 1,310,452 + SO1261 0.44 -2.1 1,310,452 + SO1261 0.44 -1.2 1,310,460 - SO1261 0.45 +2.1 1,310,460 - SO1261 0.45 -0.5 1,310,460 - SO1261 0.45 +0.7 1,310,460 - SO1261 0.45 -2.0 1,310,462 + SO1261 0.45 -1.0 1,310,462 + SO1261 0.45 +0.4 1,310,585 + SO1261 0.59 -1.2 1,310,585 + SO1261 0.59 +0.3 1,310,716 + SO1261 0.74 +0.0 1,310,872 + +0.4 1,310,896 + +1.0 1,310,985 - -2.3 1,310,992 - -1.1 1,311,129 + SO1262 0.20 +3.5 1,311,129 + SO1262 0.20 +1.2 1,311,137 - SO1262 0.21 +0.9 1,311,137 - SO1262 0.21 +0.6 1,311,137 - SO1262 0.21 -0.9 1,311,154 + SO1262 0.25 +0.6 1,311,154 + SO1262 0.25 -1.8 1,311,154 + SO1262 0.25 +1.5 1,311,162 - SO1262 0.26 -0.7 1,311,184 - SO1262 0.30 +0.5 1,311,186 + SO1262 0.31 +0.3 1,311,470 + SO1262 0.86 -0.7 1,311,485 + SO1262 0.89 +1.2 1,311,497 + -0.3 1,311,552 - +0.8 1,311,802 + +0.1 1,311,812 + +0.6 1,311,812 + -0.9 1,311,812 + +0.2 1,311,899 + +1.0 1,311,940 - +0.6 1,312,042 + SO1264 0.12 -0.1 1,312,080 - SO1264 0.15 +0.2 1,312,080 - SO1264 0.15 -1.6 1,312,110 - SO1264 0.17 -1.9 1,312,163 + SO1264 0.21 -1.0 1,312,183 + SO1264 0.23 -0.4 1,312,183 + SO1264 0.23 +1.5 1,312,191 - SO1264 0.23 -3.0 1,312,191 - SO1264 0.23 +1.2 1,312,191 - SO1264 0.23 -0.3 1,312,226 - SO1264 0.26 -1.3 1,312,228 + SO1264 0.26 -2.3 1,312,261 + SO1264 0.29 +1.0 1,312,460 + SO1264 0.44 +0.7
Or see this region's nucleotide sequence