Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1250 and SO1251 are separated by 103 nucleotides SO1251 and SO1252 are separated by 3 nucleotides SO1252 and SO1254 are separated by 448 nucleotides
SO1250: SO1250 - conserved hypothetical protein (NCBI ptt file), at 1,300,789 to 1,301,319
SO1250
SO1251: SO1251 - ferredoxin, 4Fe-4S (NCBI ptt file), at 1,301,423 to 1,301,674
SO1251
SO1252: SO1252 - peptidase, U32 family (NCBI ptt file), at 1,301,678 to 1,303,045
SO1252
SO1254: SO1254 - conserved hypothetical protein (NCBI ptt file), at 1,303,494 to 1,304,246
SO1254
Position (kb)
1301
1302
1303
1304 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1300.691 kb on - strand at 1300.691 kb on - strand at 1300.691 kb on - strand at 1300.781 kb on + strand at 1300.846 kb on - strand, within SO1250 at 1300.846 kb on - strand, within SO1250 at 1300.874 kb on + strand, within SO1250 at 1301.671 kb on - strand at 1301.675 kb on + strand at 1301.675 kb on + strand at 1301.675 kb on + strand at 1301.675 kb on + strand at 1301.675 kb on + strand at 1301.683 kb on - strand at 1301.683 kb on - strand at 1301.700 kb on + strand at 1301.705 kb on + strand at 1301.800 kb on - strand at 1301.800 kb on - strand at 1301.800 kb on - strand at 1301.943 kb on - strand, within SO1252 at 1302.058 kb on + strand, within SO1252 at 1302.058 kb on + strand, within SO1252 at 1302.227 kb on + strand, within SO1252 at 1302.246 kb on + strand, within SO1252 at 1302.395 kb on - strand, within SO1252 at 1302.508 kb on + strand, within SO1252 at 1302.531 kb on + strand, within SO1252 at 1302.531 kb on + strand, within SO1252 at 1302.531 kb on + strand, within SO1252 at 1302.531 kb on + strand, within SO1252 at 1302.533 kb on - strand, within SO1252 at 1302.533 kb on - strand, within SO1252 at 1302.539 kb on - strand, within SO1252 at 1302.539 kb on - strand, within SO1252 at 1302.689 kb on + strand, within SO1252 at 1302.692 kb on - strand, within SO1252 at 1302.767 kb on - strand, within SO1252 at 1302.767 kb on - strand, within SO1252 at 1302.767 kb on - strand, within SO1252 at 1302.824 kb on - strand, within SO1252 at 1302.934 kb on + strand at 1302.934 kb on + strand at 1302.934 kb on + strand at 1302.934 kb on + strand at 1302.942 kb on - strand at 1302.942 kb on - strand at 1302.942 kb on - strand at 1302.942 kb on - strand at 1303.035 kb on + strand at 1303.369 kb on - strand at 1303.406 kb on + strand at 1303.419 kb on + strand at 1303.489 kb on - strand at 1303.509 kb on + strand at 1303.509 kb on + strand at 1303.509 kb on + strand at 1303.509 kb on + strand at 1303.517 kb on - strand at 1303.517 kb on - strand at 1303.517 kb on - strand at 1303.524 kb on + strand at 1303.532 kb on - strand at 1303.565 kb on - strand at 1303.613 kb on - strand, within SO1254 at 1303.681 kb on - strand, within SO1254 at 1303.707 kb on - strand, within SO1254 at 1303.741 kb on - strand, within SO1254 at 1303.846 kb on + strand, within SO1254 at 1303.846 kb on + strand, within SO1254 at 1303.846 kb on + strand, within SO1254 at 1303.853 kb on - strand, within SO1254 at 1303.868 kb on - strand, within SO1254 at 1303.962 kb on + strand, within SO1254 at 1303.971 kb on + strand, within SO1254 at 1303.971 kb on + strand, within SO1254 at 1303.971 kb on + strand, within SO1254 at 1303.971 kb on + strand, within SO1254 at 1303.971 kb on + strand, within SO1254 at 1303.971 kb on + strand, within SO1254 at 1303.971 kb on + strand, within SO1254 at 1303.977 kb on - strand, within SO1254 at 1303.979 kb on - strand, within SO1254 at 1303.979 kb on - strand, within SO1254 at 1303.994 kb on - strand, within SO1254 at 1303.994 kb on - strand, within SO1254 at 1304.033 kb on - strand, within SO1254
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,300,691 - +0.7 1,300,691 - +0.4 1,300,691 - +0.3 1,300,781 + -0.3 1,300,846 - SO1250 0.11 -1.6 1,300,846 - SO1250 0.11 -0.2 1,300,874 + SO1250 0.16 +2.0 1,301,671 - +2.8 1,301,675 + -2.3 1,301,675 + +1.0 1,301,675 + -0.3 1,301,675 + -1.3 1,301,675 + -1.4 1,301,683 - -0.0 1,301,683 - +1.8 1,301,700 + +1.1 1,301,705 + +1.6 1,301,800 - +0.1 1,301,800 - -0.3 1,301,800 - +2.1 1,301,943 - SO1252 0.19 +0.4 1,302,058 + SO1252 0.28 +4.0 1,302,058 + SO1252 0.28 +1.2 1,302,227 + SO1252 0.40 +0.9 1,302,246 + SO1252 0.42 -0.2 1,302,395 - SO1252 0.52 -1.7 1,302,508 + SO1252 0.61 -2.7 1,302,531 + SO1252 0.62 +1.3 1,302,531 + SO1252 0.62 -1.0 1,302,531 + SO1252 0.62 +2.5 1,302,531 + SO1252 0.62 -1.6 1,302,533 - SO1252 0.62 +0.2 1,302,533 - SO1252 0.62 +0.9 1,302,539 - SO1252 0.63 +2.1 1,302,539 - SO1252 0.63 +1.2 1,302,689 + SO1252 0.74 -0.1 1,302,692 - SO1252 0.74 -1.1 1,302,767 - SO1252 0.80 -0.7 1,302,767 - SO1252 0.80 +2.3 1,302,767 - SO1252 0.80 -1.5 1,302,824 - SO1252 0.84 +0.6 1,302,934 + +1.1 1,302,934 + +1.2 1,302,934 + -0.3 1,302,934 + +1.6 1,302,942 - -0.7 1,302,942 - +2.2 1,302,942 - +2.3 1,302,942 - +1.1 1,303,035 + -2.0 1,303,369 - -2.4 1,303,406 + -1.8 1,303,419 + +0.4 1,303,489 - -2.3 1,303,509 + +0.3 1,303,509 + -0.8 1,303,509 + -0.4 1,303,509 + +1.4 1,303,517 - -0.0 1,303,517 - -0.1 1,303,517 - +0.8 1,303,524 + -0.7 1,303,532 - -0.1 1,303,565 - -2.3 1,303,613 - SO1254 0.16 +0.1 1,303,681 - SO1254 0.25 -0.3 1,303,707 - SO1254 0.28 -0.1 1,303,741 - SO1254 0.33 +1.6 1,303,846 + SO1254 0.47 +0.7 1,303,846 + SO1254 0.47 -0.4 1,303,846 + SO1254 0.47 +0.0 1,303,853 - SO1254 0.48 -0.1 1,303,868 - SO1254 0.50 -2.1 1,303,962 + SO1254 0.62 -1.3 1,303,971 + SO1254 0.63 +0.2 1,303,971 + SO1254 0.63 +1.0 1,303,971 + SO1254 0.63 +1.7 1,303,971 + SO1254 0.63 +0.4 1,303,971 + SO1254 0.63 -0.3 1,303,971 + SO1254 0.63 +0.7 1,303,971 + SO1254 0.63 -2.6 1,303,977 - SO1254 0.64 -2.6 1,303,979 - SO1254 0.64 +2.1 1,303,979 - SO1254 0.64 +1.3 1,303,994 - SO1254 0.66 -1.8 1,303,994 - SO1254 0.66 +1.6 1,304,033 - SO1254 0.72 +0.4
Or see this region's nucleotide sequence