Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1247 and SO1248 are separated by 47 nucleotides SO1248 and SO1249 are separated by 33 nucleotides
SO1247: SO1247 - hypothetical protein (NCBI ptt file), at 1,298,063 to 1,298,698
SO1247
SO1248: SO1248 - hypothetical peptidase (NCBI ptt file), at 1,298,746 to 1,299,615
SO1248
SO1249: SO1249 - peptidase, U32 family (NCBI ptt file), at 1,299,649 to 1,300,650
SO1249
Position (kb)
1298
1299
1300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1297.941 kb on - strand at 1298.025 kb on + strand at 1298.033 kb on - strand at 1298.087 kb on - strand at 1298.163 kb on + strand, within SO1247 at 1298.320 kb on - strand, within SO1247 at 1298.322 kb on + strand, within SO1247 at 1298.330 kb on - strand, within SO1247 at 1298.343 kb on + strand, within SO1247 at 1298.500 kb on + strand, within SO1247 at 1298.613 kb on + strand, within SO1247 at 1298.642 kb on + strand at 1298.723 kb on + strand at 1298.723 kb on + strand at 1298.731 kb on - strand at 1298.786 kb on + strand at 1298.794 kb on + strand at 1298.794 kb on - strand at 1298.794 kb on - strand at 1298.794 kb on - strand at 1298.794 kb on - strand at 1298.860 kb on + strand, within SO1248 at 1298.915 kb on + strand, within SO1248 at 1298.979 kb on + strand, within SO1248 at 1298.979 kb on + strand, within SO1248 at 1298.987 kb on - strand, within SO1248 at 1299.127 kb on - strand, within SO1248 at 1299.241 kb on + strand, within SO1248 at 1299.241 kb on + strand, within SO1248 at 1299.459 kb on + strand, within SO1248 at 1299.459 kb on + strand, within SO1248 at 1299.459 kb on + strand, within SO1248 at 1299.467 kb on - strand, within SO1248 at 1299.592 kb on - strand at 1299.815 kb on - strand, within SO1249 at 1299.876 kb on + strand, within SO1249 at 1299.894 kb on - strand, within SO1249 at 1299.942 kb on + strand, within SO1249 at 1300.044 kb on - strand, within SO1249 at 1300.049 kb on - strand, within SO1249 at 1300.191 kb on + strand, within SO1249 at 1300.228 kb on + strand, within SO1249 at 1300.228 kb on + strand, within SO1249 at 1300.228 kb on + strand, within SO1249 at 1300.228 kb on + strand, within SO1249 at 1300.228 kb on + strand, within SO1249 at 1300.228 kb on + strand, within SO1249 at 1300.236 kb on - strand, within SO1249 at 1300.236 kb on - strand, within SO1249 at 1300.246 kb on - strand, within SO1249 at 1300.246 kb on - strand, within SO1249 at 1300.300 kb on - strand, within SO1249 at 1300.310 kb on - strand, within SO1249 at 1300.589 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,297,941 - +2.7 1,298,025 + +1.9 1,298,033 - +1.5 1,298,087 - +2.7 1,298,163 + SO1247 0.16 +2.2 1,298,320 - SO1247 0.40 -0.8 1,298,322 + SO1247 0.41 -1.2 1,298,330 - SO1247 0.42 +2.4 1,298,343 + SO1247 0.44 +1.0 1,298,500 + SO1247 0.69 +0.8 1,298,613 + SO1247 0.86 +1.7 1,298,642 + -0.2 1,298,723 + +1.2 1,298,723 + +0.4 1,298,731 - -0.4 1,298,786 + +0.9 1,298,794 + +2.6 1,298,794 - +0.1 1,298,794 - -0.4 1,298,794 - -3.3 1,298,794 - -1.6 1,298,860 + SO1248 0.13 +0.3 1,298,915 + SO1248 0.19 +1.4 1,298,979 + SO1248 0.27 -0.8 1,298,979 + SO1248 0.27 -2.1 1,298,987 - SO1248 0.28 +0.5 1,299,127 - SO1248 0.44 -1.2 1,299,241 + SO1248 0.57 +5.2 1,299,241 + SO1248 0.57 +0.5 1,299,459 + SO1248 0.82 +1.4 1,299,459 + SO1248 0.82 -1.5 1,299,459 + SO1248 0.82 -0.3 1,299,467 - SO1248 0.83 -0.1 1,299,592 - +0.7 1,299,815 - SO1249 0.17 +1.3 1,299,876 + SO1249 0.23 +0.9 1,299,894 - SO1249 0.24 +0.2 1,299,942 + SO1249 0.29 -0.5 1,300,044 - SO1249 0.39 +1.5 1,300,049 - SO1249 0.40 -0.8 1,300,191 + SO1249 0.54 +1.1 1,300,228 + SO1249 0.58 +1.9 1,300,228 + SO1249 0.58 +1.1 1,300,228 + SO1249 0.58 -0.7 1,300,228 + SO1249 0.58 +0.6 1,300,228 + SO1249 0.58 +0.1 1,300,228 + SO1249 0.58 +0.7 1,300,236 - SO1249 0.59 -0.5 1,300,236 - SO1249 0.59 -0.5 1,300,246 - SO1249 0.60 -0.9 1,300,246 - SO1249 0.60 +0.6 1,300,300 - SO1249 0.65 -0.1 1,300,310 - SO1249 0.66 +1.2 1,300,589 + +2.9
Or see this region's nucleotide sequence