Strain Fitness in Shewanella oneidensis MR-1 around SO1169

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmrdA and SO1169 are separated by 63 nucleotidesSO1169 and SO1170 overlap by 1 nucleotidesSO1170 and nad are separated by 107 nucleotides SO1168: mrdA - penicillin-binding protein 2 (NCBI ptt file), at 1,210,703 to 1,212,559 mrdA SO1169: SO1169 - conserved hypothetical protein TIGR00246 (NCBI ptt file), at 1,212,623 to 1,213,093 SO1169 SO1170: SO1170 - iojap domain protein (NCBI ptt file), at 1,213,093 to 1,213,422 SO1170 SO1171: nad - nicotinate (nicotinamide) nucleotide adenylyltransferase (NCBI ptt file), at 1,213,530 to 1,214,168 nad Position (kb) 1212 1213 1214Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 1212.618 kb on - strandat 1212.676 kb on - strand, within SO1169at 1212.676 kb on - strand, within SO1169at 1212.720 kb on - strand, within SO1169at 1212.721 kb on - strand, within SO1169at 1212.772 kb on - strand, within SO1169at 1212.849 kb on - strand, within SO1169at 1212.879 kb on - strand, within SO1169at 1212.902 kb on - strand, within SO1169at 1213.012 kb on - strand, within SO1169at 1213.055 kb on - strandat 1213.149 kb on - strand, within SO1170at 1213.434 kb on - strandat 1213.457 kb on + strandat 1213.466 kb on + strandat 1213.474 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,212,618 - +0.5
1,212,676 - SO1169 0.11 +2.9
1,212,676 - SO1169 0.11 -1.8
1,212,720 - SO1169 0.21 +3.2
1,212,721 - SO1169 0.21 +1.5
1,212,772 - SO1169 0.32 +0.5
1,212,849 - SO1169 0.48 +3.6
1,212,879 - SO1169 0.54 +4.9
1,212,902 - SO1169 0.59 +3.1
1,213,012 - SO1169 0.83 -2.5
1,213,055 - +1.2
1,213,149 - SO1170 0.17 +0.6
1,213,434 - +3.9
1,213,457 + -1.5
1,213,466 + -0.4
1,213,474 - +1.7

Or see this region's nucleotide sequence