Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt carB and SO1143 are separated by 72 nucleotides SO1143 and SO1144 are separated by 296 nucleotides
SO1142: carB - carbamoyl-phosphate synthase, large subunit (NCBI ptt file), at 1,182,946 to 1,186,170
carB
SO1143: SO1143 - conserved hypothetical protein (NCBI ptt file), at 1,186,243 to 1,187,010
SO1143
SO1144: SO1144 - methyl-accepting chemotaxis protein (NCBI ptt file), at 1,187,307 to 1,188,395
SO1144
Position (kb)
1186
1187
1188 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1185.294 kb on + strand, within carB at 1185.302 kb on - strand, within carB at 1185.302 kb on - strand, within carB at 1185.334 kb on + strand, within carB at 1185.334 kb on + strand, within carB at 1185.343 kb on - strand, within carB at 1185.460 kb on + strand, within carB at 1185.471 kb on + strand, within carB at 1185.480 kb on + strand, within carB at 1185.759 kb on + strand, within carB at 1185.874 kb on + strand at 1185.897 kb on + strand at 1185.924 kb on + strand at 1185.925 kb on + strand at 1186.044 kb on + strand at 1186.044 kb on + strand at 1186.346 kb on + strand, within SO1143 at 1186.482 kb on + strand, within SO1143 at 1186.490 kb on - strand, within SO1143 at 1186.490 kb on - strand, within SO1143 at 1186.534 kb on + strand, within SO1143 at 1186.544 kb on + strand, within SO1143 at 1186.550 kb on - strand, within SO1143 at 1186.550 kb on - strand, within SO1143 at 1186.552 kb on - strand, within SO1143 at 1186.552 kb on - strand, within SO1143 at 1186.552 kb on - strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.622 kb on + strand, within SO1143 at 1186.630 kb on - strand, within SO1143 at 1186.630 kb on - strand, within SO1143 at 1186.630 kb on - strand, within SO1143 at 1186.727 kb on - strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.769 kb on + strand, within SO1143 at 1186.777 kb on - strand, within SO1143 at 1186.777 kb on - strand, within SO1143 at 1186.777 kb on - strand, within SO1143 at 1186.777 kb on - strand, within SO1143 at 1186.777 kb on - strand, within SO1143 at 1186.777 kb on - strand, within SO1143 at 1186.857 kb on + strand, within SO1143 at 1186.914 kb on - strand, within SO1143 at 1187.045 kb on + strand at 1187.045 kb on + strand at 1187.045 kb on + strand at 1187.045 kb on + strand at 1187.045 kb on + strand at 1187.045 kb on + strand at 1187.053 kb on - strand at 1187.053 kb on - strand at 1187.053 kb on - strand at 1187.074 kb on - strand at 1187.090 kb on - strand at 1187.100 kb on + strand at 1187.100 kb on + strand at 1187.108 kb on - strand at 1187.108 kb on - strand at 1187.160 kb on - strand at 1187.226 kb on + strand at 1187.229 kb on - strand at 1187.232 kb on + strand at 1187.234 kb on - strand at 1187.234 kb on - strand at 1187.235 kb on - strand at 1187.292 kb on - strand at 1187.306 kb on - strand at 1187.441 kb on - strand, within SO1144 at 1187.610 kb on + strand, within SO1144 at 1187.610 kb on + strand, within SO1144 at 1187.610 kb on - strand, within SO1144 at 1187.618 kb on - strand, within SO1144 at 1187.880 kb on + strand, within SO1144 at 1187.903 kb on - strand, within SO1144 at 1188.010 kb on + strand, within SO1144
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,185,294 + carB SO1142 0.73 -2.8 1,185,302 - carB SO1142 0.73 -2.4 1,185,302 - carB SO1142 0.73 -3.4 1,185,334 + carB SO1142 0.74 -3.7 1,185,334 + carB SO1142 0.74 -4.9 1,185,343 - carB SO1142 0.74 -3.1 1,185,460 + carB SO1142 0.78 -3.4 1,185,471 + carB SO1142 0.78 -1.8 1,185,480 + carB SO1142 0.79 -4.0 1,185,759 + carB SO1142 0.87 -3.1 1,185,874 + -3.4 1,185,897 + -1.8 1,185,924 + -4.8 1,185,925 + -3.3 1,186,044 + -4.3 1,186,044 + -3.1 1,186,346 + SO1143 0.13 -0.4 1,186,482 + SO1143 0.31 -1.0 1,186,490 - SO1143 0.32 -0.6 1,186,490 - SO1143 0.32 +2.1 1,186,534 + SO1143 0.38 +1.9 1,186,544 + SO1143 0.39 +0.7 1,186,550 - SO1143 0.40 +1.3 1,186,550 - SO1143 0.40 -1.8 1,186,552 - SO1143 0.40 +0.3 1,186,552 - SO1143 0.40 +0.4 1,186,552 - SO1143 0.40 +1.3 1,186,622 + SO1143 0.49 +2.0 1,186,622 + SO1143 0.49 -0.1 1,186,622 + SO1143 0.49 -0.4 1,186,622 + SO1143 0.49 +1.2 1,186,622 + SO1143 0.49 +0.9 1,186,622 + SO1143 0.49 +2.8 1,186,622 + SO1143 0.49 -0.8 1,186,622 + SO1143 0.49 -0.1 1,186,622 + SO1143 0.49 +1.6 1,186,630 - SO1143 0.50 +2.1 1,186,630 - SO1143 0.50 -0.7 1,186,630 - SO1143 0.50 -1.2 1,186,727 - SO1143 0.63 -1.2 1,186,769 + SO1143 0.68 -1.5 1,186,769 + SO1143 0.68 -0.8 1,186,769 + SO1143 0.68 -1.4 1,186,769 + SO1143 0.68 +0.8 1,186,769 + SO1143 0.68 -0.5 1,186,769 + SO1143 0.68 +0.0 1,186,769 + SO1143 0.68 +1.0 1,186,769 + SO1143 0.68 +0.1 1,186,769 + SO1143 0.68 -2.8 1,186,777 - SO1143 0.70 -0.2 1,186,777 - SO1143 0.70 -1.2 1,186,777 - SO1143 0.70 -2.4 1,186,777 - SO1143 0.70 -1.3 1,186,777 - SO1143 0.70 -1.8 1,186,777 - SO1143 0.70 -0.2 1,186,857 + SO1143 0.80 +1.2 1,186,914 - SO1143 0.87 +2.6 1,187,045 + -3.2 1,187,045 + -1.7 1,187,045 + +0.4 1,187,045 + -0.8 1,187,045 + -0.8 1,187,045 + +1.8 1,187,053 - +2.0 1,187,053 - -2.0 1,187,053 - -0.1 1,187,074 - -1.5 1,187,090 - -0.4 1,187,100 + +1.4 1,187,100 + +1.1 1,187,108 - +1.0 1,187,108 - -2.6 1,187,160 - -1.1 1,187,226 + +1.0 1,187,229 - -1.7 1,187,232 + -0.1 1,187,234 - +0.4 1,187,234 - -2.1 1,187,235 - -0.2 1,187,292 - -0.9 1,187,306 - -1.2 1,187,441 - SO1144 0.12 +1.5 1,187,610 + SO1144 0.28 -1.1 1,187,610 + SO1144 0.28 -2.4 1,187,610 - SO1144 0.28 -0.7 1,187,618 - SO1144 0.29 -0.3 1,187,880 + SO1144 0.53 +0.2 1,187,903 - SO1144 0.55 -1.7 1,188,010 + SO1144 0.65 +1.6
Or see this region's nucleotide sequence