Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1129 and SO1130 are separated by 59 nucleotides SO1130 and SO1132 are separated by 217 nucleotides SO1132 and SO1133 are separated by 14 nucleotides SO1133 and SO1134 are separated by 59 nucleotides
SO1129: SO1129 - ISSod1, transposase OrfB (NCBI ptt file), at 1,172,848 to 1,173,657
SO1129
SO1130: SO1130 - ISSod1, transposase OrfA (NCBI ptt file), at 1,173,717 to 1,174,007
SO1130
SO1132: SO1132 - hypothetical protein (NCBI ptt file), at 1,174,225 to 1,174,659
SO1132
SO1133: SO1133 - ISSod1, transposase OrfA (NCBI ptt file), at 1,174,674 to 1,174,964
SO1133
SO1134: SO1134 - ISSod1, transposase OrfB (NCBI ptt file), at 1,175,024 to 1,175,833
SO1134
Position (kb)
1174
1175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1174.060 kb on + strand at 1174.060 kb on + strand at 1174.060 kb on + strand at 1174.060 kb on + strand at 1174.060 kb on + strand at 1174.060 kb on + strand at 1174.060 kb on + strand at 1174.115 kb on - strand at 1174.128 kb on + strand at 1174.160 kb on + strand at 1174.223 kb on - strand at 1174.323 kb on + strand, within SO1132 at 1174.323 kb on + strand, within SO1132 at 1174.331 kb on - strand, within SO1132 at 1174.331 kb on - strand, within SO1132 at 1174.541 kb on - strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.599 kb on + strand, within SO1132 at 1174.606 kb on - strand, within SO1132 at 1174.606 kb on - strand, within SO1132 at 1174.606 kb on - strand, within SO1132 at 1174.607 kb on - strand, within SO1132 at 1174.607 kb on - strand, within SO1132 at 1174.607 kb on - strand, within SO1132 at 1174.616 kb on - strand at 1174.621 kb on - strand at 1174.621 kb on - strand at 1174.621 kb on - strand at 1174.621 kb on - strand at 1174.621 kb on - strand at 1174.621 kb on - strand at 1174.621 kb on - strand at 1174.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,174,060 + -0.1 1,174,060 + +0.3 1,174,060 + +2.0 1,174,060 + -0.9 1,174,060 + +1.0 1,174,060 + -3.2 1,174,060 + +0.2 1,174,115 - +0.1 1,174,128 + +0.4 1,174,160 + +0.1 1,174,223 - +1.5 1,174,323 + SO1132 0.23 -1.1 1,174,323 + SO1132 0.23 +2.3 1,174,331 - SO1132 0.24 +0.4 1,174,331 - SO1132 0.24 +0.9 1,174,541 - SO1132 0.73 +1.0 1,174,599 + SO1132 0.86 -2.2 1,174,599 + SO1132 0.86 -1.7 1,174,599 + SO1132 0.86 -2.9 1,174,599 + SO1132 0.86 -0.4 1,174,599 + SO1132 0.86 +0.1 1,174,599 + SO1132 0.86 -0.4 1,174,599 + SO1132 0.86 -1.1 1,174,599 + SO1132 0.86 +0.4 1,174,606 - SO1132 0.88 +0.3 1,174,606 - SO1132 0.88 +0.4 1,174,606 - SO1132 0.88 -0.6 1,174,607 - SO1132 0.88 +1.0 1,174,607 - SO1132 0.88 -0.9 1,174,607 - SO1132 0.88 -0.7 1,174,616 - +0.8 1,174,621 - -0.5 1,174,621 - +1.2 1,174,621 - -1.6 1,174,621 - +0.5 1,174,621 - -0.1 1,174,621 - +3.9 1,174,621 - +0.2 1,174,621 - -0.2
Or see this region's nucleotide sequence