Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fkpA and SO1066 are separated by 563 nucleotides SO1066 and SO1067 are separated by 133 nucleotides SO1067 and SO1068 are separated by 108 nucleotides
SO1065: fkpA - FKBP-type peptidyl-prolyl cis-trans isomerase FkpA (NCBI ptt file), at 1,104,192 to 1,104,959
fkpA
SO1066: SO1066 - extracellular nuclease (NCBI ptt file), at 1,105,523 to 1,108,138
SO1066
SO1067: SO1067 - hypothetical protein (NCBI ptt file), at 1,108,272 to 1,108,727
SO1067
SO1068: SO1068 - conserved hypothetical protein (NCBI ptt file), at 1,108,836 to 1,109,273
SO1068
Position (kb)
1105
1106
1107
1108
1109 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1104.529 kb on - strand, within fkpA at 1104.596 kb on + strand, within fkpA at 1104.599 kb on - strand, within fkpA at 1104.650 kb on + strand, within fkpA at 1104.668 kb on - strand, within fkpA at 1104.730 kb on + strand, within fkpA at 1104.768 kb on + strand, within fkpA at 1104.776 kb on - strand, within fkpA at 1104.787 kb on - strand, within fkpA at 1104.821 kb on - strand, within fkpA at 1104.865 kb on + strand, within fkpA at 1104.870 kb on + strand, within fkpA at 1104.880 kb on + strand, within fkpA at 1104.888 kb on - strand at 1104.888 kb on - strand at 1104.945 kb on - strand at 1104.946 kb on - strand at 1104.964 kb on - strand at 1105.025 kb on + strand at 1105.035 kb on - strand at 1105.043 kb on - strand at 1105.047 kb on + strand at 1105.062 kb on + strand at 1105.062 kb on + strand at 1105.158 kb on + strand at 1105.164 kb on + strand at 1105.166 kb on - strand at 1105.172 kb on - strand at 1105.172 kb on - strand at 1105.172 kb on - strand at 1105.205 kb on - strand at 1105.290 kb on - strand at 1105.290 kb on - strand at 1105.296 kb on + strand at 1105.590 kb on + strand at 1105.603 kb on - strand at 1105.616 kb on + strand at 1105.616 kb on + strand at 1105.616 kb on + strand at 1105.616 kb on + strand at 1105.616 kb on + strand at 1105.616 kb on + strand at 1105.616 kb on + strand at 1105.624 kb on - strand at 1105.624 kb on - strand at 1105.624 kb on - strand at 1105.624 kb on - strand at 1105.659 kb on - strand at 1105.679 kb on - strand at 1105.686 kb on + strand at 1105.756 kb on - strand at 1105.756 kb on - strand at 1105.770 kb on - strand at 1105.786 kb on + strand, within SO1066 at 1105.806 kb on - strand, within SO1066 at 1105.810 kb on + strand, within SO1066 at 1105.810 kb on + strand, within SO1066 at 1105.810 kb on + strand, within SO1066 at 1105.817 kb on + strand, within SO1066 at 1105.818 kb on - strand, within SO1066 at 1105.910 kb on + strand, within SO1066 at 1105.917 kb on + strand, within SO1066 at 1105.943 kb on + strand, within SO1066 at 1105.959 kb on - strand, within SO1066 at 1105.992 kb on - strand, within SO1066 at 1106.006 kb on + strand, within SO1066 at 1106.006 kb on - strand, within SO1066 at 1106.030 kb on + strand, within SO1066 at 1106.049 kb on + strand, within SO1066 at 1106.113 kb on + strand, within SO1066 at 1106.129 kb on - strand, within SO1066 at 1106.139 kb on - strand, within SO1066 at 1106.175 kb on + strand, within SO1066 at 1106.189 kb on - strand, within SO1066 at 1106.242 kb on + strand, within SO1066 at 1106.344 kb on + strand, within SO1066 at 1106.344 kb on + strand, within SO1066 at 1106.344 kb on + strand, within SO1066 at 1106.344 kb on + strand, within SO1066 at 1106.352 kb on - strand, within SO1066 at 1106.352 kb on - strand, within SO1066 at 1106.509 kb on + strand, within SO1066 at 1106.593 kb on - strand, within SO1066 at 1106.600 kb on + strand, within SO1066 at 1106.620 kb on + strand, within SO1066 at 1106.633 kb on - strand, within SO1066 at 1106.753 kb on + strand, within SO1066 at 1106.753 kb on + strand, within SO1066 at 1106.761 kb on - strand, within SO1066 at 1106.761 kb on - strand, within SO1066 at 1106.761 kb on - strand, within SO1066 at 1106.792 kb on + strand, within SO1066 at 1106.906 kb on + strand, within SO1066 at 1106.925 kb on + strand, within SO1066 at 1106.989 kb on + strand, within SO1066 at 1107.172 kb on + strand, within SO1066 at 1107.182 kb on - strand, within SO1066 at 1107.247 kb on + strand, within SO1066 at 1107.247 kb on + strand, within SO1066 at 1107.268 kb on - strand, within SO1066 at 1107.301 kb on - strand, within SO1066 at 1107.317 kb on + strand, within SO1066 at 1107.317 kb on + strand, within SO1066 at 1107.326 kb on + strand, within SO1066 at 1107.335 kb on - strand, within SO1066 at 1107.352 kb on + strand, within SO1066 at 1107.354 kb on - strand, within SO1066 at 1107.386 kb on + strand, within SO1066 at 1107.386 kb on + strand, within SO1066 at 1107.386 kb on + strand, within SO1066 at 1107.386 kb on + strand, within SO1066 at 1107.394 kb on - strand, within SO1066 at 1107.394 kb on - strand, within SO1066 at 1107.398 kb on + strand, within SO1066 at 1107.405 kb on + strand, within SO1066 at 1107.413 kb on - strand, within SO1066 at 1107.461 kb on + strand, within SO1066 at 1107.486 kb on - strand, within SO1066 at 1107.523 kb on + strand, within SO1066 at 1107.560 kb on + strand, within SO1066 at 1107.722 kb on + strand, within SO1066 at 1107.730 kb on + strand, within SO1066 at 1107.731 kb on + strand, within SO1066 at 1107.754 kb on + strand, within SO1066 at 1107.792 kb on + strand, within SO1066 at 1107.811 kb on - strand, within SO1066 at 1107.855 kb on + strand, within SO1066 at 1107.898 kb on - strand at 1107.912 kb on + strand at 1107.912 kb on + strand at 1107.912 kb on + strand at 1107.920 kb on - strand at 1107.922 kb on + strand at 1107.930 kb on - strand at 1107.986 kb on + strand at 1107.988 kb on - strand at 1108.049 kb on - strand at 1108.073 kb on + strand at 1108.106 kb on + strand at 1108.170 kb on - strand at 1108.213 kb on + strand at 1108.263 kb on - strand at 1108.263 kb on - strand at 1108.285 kb on + strand at 1108.320 kb on - strand, within SO1067 at 1108.410 kb on - strand, within SO1067 at 1108.410 kb on - strand, within SO1067 at 1108.434 kb on - strand, within SO1067 at 1108.434 kb on - strand, within SO1067 at 1108.503 kb on + strand, within SO1067 at 1108.648 kb on + strand, within SO1067 at 1108.736 kb on - strand at 1108.861 kb on + strand at 1108.869 kb on - strand at 1108.869 kb on - strand at 1108.869 kb on - strand at 1108.914 kb on + strand, within SO1068 at 1108.921 kb on - strand, within SO1068 at 1108.951 kb on - strand, within SO1068 at 1109.005 kb on + strand, within SO1068 at 1109.025 kb on + strand, within SO1068
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,104,529 - fkpA SO1065 0.44 +0.6 1,104,596 + fkpA SO1065 0.53 -0.8 1,104,599 - fkpA SO1065 0.53 -2.9 1,104,650 + fkpA SO1065 0.60 +0.0 1,104,668 - fkpA SO1065 0.62 +1.7 1,104,730 + fkpA SO1065 0.70 -0.9 1,104,768 + fkpA SO1065 0.75 +1.0 1,104,776 - fkpA SO1065 0.76 +0.1 1,104,787 - fkpA SO1065 0.77 +1.2 1,104,821 - fkpA SO1065 0.82 -1.0 1,104,865 + fkpA SO1065 0.88 -0.9 1,104,870 + fkpA SO1065 0.88 -1.1 1,104,880 + fkpA SO1065 0.90 +0.5 1,104,888 - -0.6 1,104,888 - +2.8 1,104,945 - +1.2 1,104,946 - -0.8 1,104,964 - +0.5 1,105,025 + -0.3 1,105,035 - -2.9 1,105,043 - -2.1 1,105,047 + +1.7 1,105,062 + +0.7 1,105,062 + -0.1 1,105,158 + -2.4 1,105,164 + +1.6 1,105,166 - +1.1 1,105,172 - -0.6 1,105,172 - +0.6 1,105,172 - -0.4 1,105,205 - -1.5 1,105,290 - +0.1 1,105,290 - -1.3 1,105,296 + +1.7 1,105,590 + -0.9 1,105,603 - +2.9 1,105,616 + -0.9 1,105,616 + -0.4 1,105,616 + +0.3 1,105,616 + +1.4 1,105,616 + +0.7 1,105,616 + +0.6 1,105,616 + +2.5 1,105,624 - -0.2 1,105,624 - -0.9 1,105,624 - -0.2 1,105,624 - -0.4 1,105,659 - +1.3 1,105,679 - +1.5 1,105,686 + -0.9 1,105,756 - +0.6 1,105,756 - +0.8 1,105,770 - -0.5 1,105,786 + SO1066 0.10 +0.5 1,105,806 - SO1066 0.11 +0.5 1,105,810 + SO1066 0.11 +0.1 1,105,810 + SO1066 0.11 +2.3 1,105,810 + SO1066 0.11 -2.9 1,105,817 + SO1066 0.11 +1.1 1,105,818 - SO1066 0.11 -1.7 1,105,910 + SO1066 0.15 +1.7 1,105,917 + SO1066 0.15 +0.9 1,105,943 + SO1066 0.16 -0.8 1,105,959 - SO1066 0.17 -0.3 1,105,992 - SO1066 0.18 -0.5 1,106,006 + SO1066 0.18 -0.9 1,106,006 - SO1066 0.18 -1.3 1,106,030 + SO1066 0.19 -0.1 1,106,049 + SO1066 0.20 -0.4 1,106,113 + SO1066 0.23 +2.4 1,106,129 - SO1066 0.23 +2.4 1,106,139 - SO1066 0.24 -1.5 1,106,175 + SO1066 0.25 -0.8 1,106,189 - SO1066 0.25 -0.9 1,106,242 + SO1066 0.27 -2.3 1,106,344 + SO1066 0.31 +1.4 1,106,344 + SO1066 0.31 -1.2 1,106,344 + SO1066 0.31 +0.3 1,106,344 + SO1066 0.31 +1.6 1,106,352 - SO1066 0.32 -0.7 1,106,352 - SO1066 0.32 +1.2 1,106,509 + SO1066 0.38 -1.2 1,106,593 - SO1066 0.41 -0.1 1,106,600 + SO1066 0.41 +1.5 1,106,620 + SO1066 0.42 -4.6 1,106,633 - SO1066 0.42 +0.2 1,106,753 + SO1066 0.47 -1.0 1,106,753 + SO1066 0.47 -0.8 1,106,761 - SO1066 0.47 -0.2 1,106,761 - SO1066 0.47 +1.0 1,106,761 - SO1066 0.47 +1.0 1,106,792 + SO1066 0.49 -0.3 1,106,906 + SO1066 0.53 -0.3 1,106,925 + SO1066 0.54 -1.8 1,106,989 + SO1066 0.56 +0.0 1,107,172 + SO1066 0.63 +0.1 1,107,182 - SO1066 0.63 +1.4 1,107,247 + SO1066 0.66 +0.9 1,107,247 + SO1066 0.66 +0.5 1,107,268 - SO1066 0.67 -0.4 1,107,301 - SO1066 0.68 +1.2 1,107,317 + SO1066 0.69 -0.0 1,107,317 + SO1066 0.69 -3.5 1,107,326 + SO1066 0.69 -2.0 1,107,335 - SO1066 0.69 -1.7 1,107,352 + SO1066 0.70 +1.8 1,107,354 - SO1066 0.70 -2.2 1,107,386 + SO1066 0.71 -2.3 1,107,386 + SO1066 0.71 -0.6 1,107,386 + SO1066 0.71 -0.0 1,107,386 + SO1066 0.71 -0.8 1,107,394 - SO1066 0.72 +0.0 1,107,394 - SO1066 0.72 +0.4 1,107,398 + SO1066 0.72 +2.2 1,107,405 + SO1066 0.72 -0.5 1,107,413 - SO1066 0.72 +0.9 1,107,461 + SO1066 0.74 -0.5 1,107,486 - SO1066 0.75 +0.7 1,107,523 + SO1066 0.76 -2.5 1,107,560 + SO1066 0.78 -1.7 1,107,722 + SO1066 0.84 -1.9 1,107,730 + SO1066 0.84 -1.8 1,107,731 + SO1066 0.84 -1.1 1,107,754 + SO1066 0.85 +0.8 1,107,792 + SO1066 0.87 -0.3 1,107,811 - SO1066 0.87 -0.1 1,107,855 + SO1066 0.89 +1.1 1,107,898 - -0.3 1,107,912 + -0.4 1,107,912 + -0.3 1,107,912 + +0.7 1,107,920 - -1.4 1,107,922 + +0.0 1,107,930 - +1.2 1,107,986 + +0.1 1,107,988 - -4.6 1,108,049 - -2.0 1,108,073 + +1.5 1,108,106 + -2.8 1,108,170 - -0.1 1,108,213 + +0.2 1,108,263 - -0.0 1,108,263 - -1.5 1,108,285 + +0.7 1,108,320 - SO1067 0.11 +0.4 1,108,410 - SO1067 0.30 +0.5 1,108,410 - SO1067 0.30 -1.8 1,108,434 - SO1067 0.36 -1.2 1,108,434 - SO1067 0.36 -1.2 1,108,503 + SO1067 0.51 -0.5 1,108,648 + SO1067 0.82 +0.1 1,108,736 - -0.2 1,108,861 + -2.4 1,108,869 - +0.9 1,108,869 - +0.0 1,108,869 - -0.8 1,108,914 + SO1068 0.18 -3.4 1,108,921 - SO1068 0.19 -0.8 1,108,951 - SO1068 0.26 -0.6 1,109,005 + SO1068 0.39 +1.3 1,109,025 + SO1068 0.43 -3.3
Or see this region's nucleotide sequence