Strain Fitness in Shewanella oneidensis MR-1 around SO0997

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO0995 and SO0996 are separated by 56 nucleotidesSO0996 and SO0997 are separated by 169 nucleotidesSO0997 and SO0998 overlap by 11 nucleotidesSO0998 and pbpG are separated by 124 nucleotides SO0995: SO0995 - hypothetical protein (NCBI ptt file), at 1,027,831 to 1,028,025 SO0995 SO0996: SO0996 - glyoxalase family protein (NCBI ptt file), at 1,028,082 to 1,028,582 SO0996 SO0997: SO0997 - transcriptional regulator, LysR family (NCBI ptt file), at 1,028,752 to 1,029,684 SO0997 SO0998: SO0998 - hypothetical protein (NCBI ptt file), at 1,029,674 to 1,029,853 SO0998 SO0999: pbpG - D-alanyl-D-alanine endopeptidase (NCBI ptt file), at 1,029,978 to 1,030,943 pbpG Position (kb) 1028 1029 1030Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1027.802 kb on - strandat 1027.831 kb on - strandat 1028.101 kb on + strandat 1028.196 kb on + strand, within SO0996at 1028.223 kb on + strand, within SO0996at 1028.317 kb on - strand, within SO0996at 1028.326 kb on + strand, within SO0996at 1028.433 kb on + strand, within SO0996at 1028.433 kb on + strand, within SO0996at 1028.475 kb on - strand, within SO0996at 1028.490 kb on - strand, within SO0996at 1028.506 kb on + strand, within SO0996at 1028.514 kb on - strand, within SO0996at 1028.619 kb on + strandat 1028.640 kb on + strandat 1028.650 kb on + strandat 1028.742 kb on + strandat 1028.789 kb on + strandat 1028.803 kb on + strandat 1028.829 kb on + strandat 1028.829 kb on + strandat 1028.837 kb on - strandat 1028.849 kb on - strand, within SO0997at 1028.849 kb on - strand, within SO0997at 1028.916 kb on + strand, within SO0997at 1029.066 kb on - strand, within SO0997at 1029.098 kb on + strand, within SO0997at 1029.106 kb on - strand, within SO0997at 1029.106 kb on - strand, within SO0997at 1029.223 kb on + strand, within SO0997at 1029.223 kb on + strand, within SO0997at 1029.230 kb on - strand, within SO0997at 1029.231 kb on - strand, within SO0997at 1029.236 kb on - strand, within SO0997at 1029.557 kb on + strand, within SO0997at 1029.628 kb on - strandat 1029.632 kb on - strandat 1029.896 kb on - strandat 1029.911 kb on + strandat 1030.024 kb on + strandat 1030.032 kb on - strandat 1030.032 kb on - strandat 1030.168 kb on - strand, within pbpGat 1030.257 kb on - strand, within pbpGat 1030.285 kb on + strand, within pbpGat 1030.429 kb on - strand, within pbpGat 1030.596 kb on + strand, within pbpG

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,027,802 - +2.3
1,027,831 - +1.7
1,028,101 + +1.0
1,028,196 + SO0996 0.23 +0.8
1,028,223 + SO0996 0.28 +0.5
1,028,317 - SO0996 0.47 +2.0
1,028,326 + SO0996 0.49 +0.1
1,028,433 + SO0996 0.70 +0.9
1,028,433 + SO0996 0.70 -2.3
1,028,475 - SO0996 0.78 -0.2
1,028,490 - SO0996 0.81 -1.6
1,028,506 + SO0996 0.85 -0.1
1,028,514 - SO0996 0.86 +0.6
1,028,619 + -0.6
1,028,640 + +0.6
1,028,650 + -3.1
1,028,742 + +0.1
1,028,789 + +0.4
1,028,803 + +0.1
1,028,829 + -0.4
1,028,829 + +0.4
1,028,837 - -1.1
1,028,849 - SO0997 0.10 +0.7
1,028,849 - SO0997 0.10 +0.7
1,028,916 + SO0997 0.18 +4.6
1,029,066 - SO0997 0.34 +2.8
1,029,098 + SO0997 0.37 +0.1
1,029,106 - SO0997 0.38 -0.6
1,029,106 - SO0997 0.38 -1.2
1,029,223 + SO0997 0.50 +0.8
1,029,223 + SO0997 0.50 +1.4
1,029,230 - SO0997 0.51 -0.4
1,029,231 - SO0997 0.51 +2.0
1,029,236 - SO0997 0.52 -2.6
1,029,557 + SO0997 0.86 +2.0
1,029,628 - -3.4
1,029,632 - -0.2
1,029,896 - -0.6
1,029,911 + +0.8
1,030,024 + -2.3
1,030,032 - +1.0
1,030,032 - +0.6
1,030,168 - pbpG SO0999 0.20 +2.8
1,030,257 - pbpG SO0999 0.29 +1.0
1,030,285 + pbpG SO0999 0.32 +0.3
1,030,429 - pbpG SO0999 0.47 -1.1
1,030,596 + pbpG SO0999 0.64 +0.5

Or see this region's nucleotide sequence