Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0978 and SO0980 are separated by 191 nucleotides SO0980 and SO0981 are separated by 31 nucleotides SO0981 and SO0982 are separated by 48 nucleotides
SO0978: SO0978 - FAD-dependent glycerol-3-phosphate dehydrogenase, family protein (NCBI ptt file), at 1,008,247 to 1,009,422
SO0978
SO0980: SO0980 - RNA methyltransferase, TrmA family (NCBI ptt file), at 1,009,614 to 1,010,762
SO0980
SO0981: SO0981 - sensor histidine kinase (NCBI ptt file), at 1,010,794 to 1,011,435
SO0981
SO0982: SO0982 - ISSod1, transposase OrfA (NCBI ptt file), at 1,011,484 to 1,011,774
SO0982
Position (kb)
1009
1010
1011 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1008.647 kb on + strand, within SO0978 at 1008.655 kb on - strand, within SO0978 at 1008.661 kb on + strand, within SO0978 at 1008.734 kb on + strand, within SO0978 at 1008.758 kb on + strand, within SO0978 at 1008.843 kb on - strand, within SO0978 at 1009.004 kb on + strand, within SO0978 at 1009.053 kb on + strand, within SO0978 at 1009.070 kb on - strand, within SO0978 at 1009.190 kb on - strand, within SO0978 at 1009.216 kb on - strand, within SO0978 at 1009.259 kb on - strand, within SO0978 at 1009.274 kb on + strand, within SO0978 at 1009.282 kb on - strand, within SO0978 at 1009.282 kb on - strand, within SO0978 at 1009.282 kb on - strand, within SO0978 at 1009.325 kb on - strand at 1009.417 kb on + strand at 1009.609 kb on + strand at 1009.635 kb on - strand at 1009.761 kb on + strand, within SO0980 at 1009.770 kb on + strand, within SO0980 at 1009.819 kb on - strand, within SO0980 at 1009.821 kb on + strand, within SO0980 at 1009.828 kb on + strand, within SO0980 at 1009.833 kb on + strand, within SO0980 at 1009.864 kb on + strand, within SO0980 at 1010.063 kb on - strand, within SO0980 at 1010.063 kb on - strand, within SO0980 at 1010.158 kb on + strand, within SO0980 at 1010.270 kb on - strand, within SO0980 at 1010.270 kb on - strand, within SO0980 at 1010.275 kb on + strand, within SO0980 at 1010.283 kb on - strand, within SO0980 at 1010.359 kb on + strand, within SO0980 at 1010.359 kb on + strand, within SO0980 at 1010.359 kb on + strand, within SO0980 at 1010.372 kb on - strand, within SO0980 at 1010.377 kb on - strand, within SO0980 at 1010.445 kb on + strand, within SO0980 at 1010.517 kb on + strand, within SO0980 at 1010.563 kb on + strand, within SO0980 at 1010.563 kb on + strand, within SO0980 at 1010.581 kb on - strand, within SO0980 at 1010.769 kb on - strand at 1010.769 kb on - strand at 1010.813 kb on + strand at 1010.868 kb on - strand, within SO0981 at 1010.886 kb on - strand, within SO0981 at 1010.959 kb on - strand, within SO0981 at 1010.961 kb on + strand, within SO0981 at 1011.114 kb on + strand, within SO0981 at 1011.138 kb on - strand, within SO0981 at 1011.194 kb on + strand, within SO0981 at 1011.253 kb on + strand, within SO0981 at 1011.353 kb on + strand, within SO0981 at 1011.353 kb on + strand, within SO0981 at 1011.431 kb on - strand at 1011.431 kb on - strand at 1011.431 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,008,647 + SO0978 0.34 -1.6 1,008,655 - SO0978 0.35 -2.7 1,008,661 + SO0978 0.35 -0.6 1,008,734 + SO0978 0.41 -2.8 1,008,758 + SO0978 0.43 -2.9 1,008,843 - SO0978 0.51 -1.3 1,009,004 + SO0978 0.64 -3.3 1,009,053 + SO0978 0.69 -3.8 1,009,070 - SO0978 0.70 -3.1 1,009,190 - SO0978 0.80 -3.5 1,009,216 - SO0978 0.82 -1.5 1,009,259 - SO0978 0.86 -0.7 1,009,274 + SO0978 0.87 -4.5 1,009,282 - SO0978 0.88 +1.5 1,009,282 - SO0978 0.88 -2.7 1,009,282 - SO0978 0.88 -2.5 1,009,325 - -1.6 1,009,417 + +0.3 1,009,609 + +0.9 1,009,635 - -0.6 1,009,761 + SO0980 0.13 +1.5 1,009,770 + SO0980 0.14 -1.9 1,009,819 - SO0980 0.18 +1.3 1,009,821 + SO0980 0.18 +0.5 1,009,828 + SO0980 0.19 -0.9 1,009,833 + SO0980 0.19 +1.7 1,009,864 + SO0980 0.22 +0.2 1,010,063 - SO0980 0.39 +0.8 1,010,063 - SO0980 0.39 -0.5 1,010,158 + SO0980 0.47 +2.3 1,010,270 - SO0980 0.57 +0.9 1,010,270 - SO0980 0.57 +0.1 1,010,275 + SO0980 0.58 -1.6 1,010,283 - SO0980 0.58 +1.9 1,010,359 + SO0980 0.65 +0.2 1,010,359 + SO0980 0.65 -1.9 1,010,359 + SO0980 0.65 +1.1 1,010,372 - SO0980 0.66 +0.9 1,010,377 - SO0980 0.66 +1.8 1,010,445 + SO0980 0.72 +1.9 1,010,517 + SO0980 0.79 -1.1 1,010,563 + SO0980 0.83 -0.1 1,010,563 + SO0980 0.83 +0.0 1,010,581 - SO0980 0.84 -0.8 1,010,769 - -2.1 1,010,769 - +3.9 1,010,813 + -0.7 1,010,868 - SO0981 0.12 -0.1 1,010,886 - SO0981 0.14 -1.0 1,010,959 - SO0981 0.26 +0.0 1,010,961 + SO0981 0.26 +1.1 1,011,114 + SO0981 0.50 -0.4 1,011,138 - SO0981 0.54 +1.2 1,011,194 + SO0981 0.62 +0.4 1,011,253 + SO0981 0.71 +1.6 1,011,353 + SO0981 0.87 -1.0 1,011,353 + SO0981 0.87 +0.6 1,011,431 - +1.3 1,011,431 - +0.6 1,011,431 - +0.3
Or see this region's nucleotide sequence