Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fba and SO0934 are separated by 137 nucleotides SO0934 and nhaD are separated by 279 nucleotides
SO0933: fba - fructose-bisphosphate aldolase, class II, Calvin cycle subtype (NCBI ptt file), at 964,252 to 965,319
fba
SO0934: SO0934 - conserved hypothetical protein (NCBI ptt file), at 965,457 to 966,194
SO0934
SO0935: nhaD - Na+/H+ antiporter (NCBI ptt file), at 966,474 to 967,904
nhaD
Position (kb) 965
966
967 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 965.294 kb on + strand at 965.320 kb on + strand at 965.483 kb on + strand at 965.491 kb on - strand at 965.594 kb on + strand, within SO0934 at 965.705 kb on + strand, within SO0934 at 965.705 kb on + strand, within SO0934 at 965.705 kb on + strand, within SO0934 at 965.760 kb on - strand, within SO0934 at 965.796 kb on - strand, within SO0934 at 965.826 kb on + strand, within SO0934 at 965.826 kb on + strand, within SO0934 at 965.841 kb on - strand, within SO0934 at 965.841 kb on - strand, within SO0934 at 965.849 kb on - strand, within SO0934 at 966.175 kb on - strand at 966.541 kb on + strand at 966.565 kb on + strand at 966.617 kb on + strand, within nhaD at 966.803 kb on - strand, within nhaD at 966.861 kb on - strand, within nhaD at 966.876 kb on - strand, within nhaD at 966.946 kb on - strand, within nhaD at 966.964 kb on - strand, within nhaD at 967.120 kb on + strand, within nhaD at 967.130 kb on + strand, within nhaD at 967.130 kb on + strand, within nhaD
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 965,294 + -1.7 965,320 + -4.1 965,483 + +0.6 965,491 - +1.4 965,594 + SO0934 0.19 -0.3 965,705 + SO0934 0.34 +1.7 965,705 + SO0934 0.34 +0.7 965,705 + SO0934 0.34 +0.6 965,760 - SO0934 0.41 -0.6 965,796 - SO0934 0.46 -1.5 965,826 + SO0934 0.50 +0.5 965,826 + SO0934 0.50 -1.1 965,841 - SO0934 0.52 +0.9 965,841 - SO0934 0.52 +3.1 965,849 - SO0934 0.53 -2.7 966,175 - -1.7 966,541 + -1.6 966,565 + +0.9 966,617 + nhaD SO0935 0.10 -0.4 966,803 - nhaD SO0935 0.23 +1.5 966,861 - nhaD SO0935 0.27 +1.6 966,876 - nhaD SO0935 0.28 -1.1 966,946 - nhaD SO0935 0.33 -3.4 966,964 - nhaD SO0935 0.34 +0.1 967,120 + nhaD SO0935 0.45 +1.2 967,130 + nhaD SO0935 0.46 +1.9 967,130 + nhaD SO0935 0.46 +4.9
Or see this region's nucleotide sequence