Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gshB and SO0832 are separated by 93 nucleotides SO0832 and endA are separated by 8 nucleotides endA and SO0834 are separated by 138 nucleotides SO0834 and SO0835 are separated by 141 nucleotides
SO0831: gshB - glutathione synthetase (NCBI ptt file), at 849,536 to 850,483
gshB
SO0832: SO0832 - conserved hypothetical protein TIGR00046 (NCBI ptt file), at 850,577 to 851,308
SO0832
SO0833: endA - endonuclease I (NCBI ptt file), at 851,317 to 852,093
endA
SO0834: SO0834 - sprT protein, putative (NCBI ptt file), at 852,232 to 852,852
SO0834
SO0835: SO0835 - conserved hypothetical protein (NCBI ptt file), at 852,994 to 854,151
SO0835
Position (kb) 851
852
853 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 850.333 kb on + strand, within gshB at 850.397 kb on - strand at 850.462 kb on + strand at 850.529 kb on + strand at 850.562 kb on - strand at 850.622 kb on + strand at 850.630 kb on - strand at 850.630 kb on - strand at 850.632 kb on + strand at 850.640 kb on - strand at 850.666 kb on - strand, within SO0832 at 850.763 kb on - strand, within SO0832 at 850.768 kb on + strand, within SO0832 at 850.783 kb on + strand, within SO0832 at 851.007 kb on - strand, within SO0832 at 851.007 kb on - strand, within SO0832 at 851.064 kb on + strand, within SO0832 at 851.162 kb on + strand, within SO0832 at 851.183 kb on - strand, within SO0832 at 851.207 kb on - strand, within SO0832 at 851.218 kb on - strand, within SO0832 at 851.441 kb on + strand, within endA at 851.443 kb on - strand, within endA at 851.651 kb on + strand, within endA at 851.651 kb on + strand, within endA at 851.681 kb on - strand, within endA at 851.763 kb on - strand, within endA at 851.796 kb on - strand, within endA at 851.796 kb on - strand, within endA at 851.937 kb on - strand, within endA at 851.937 kb on - strand, within endA at 851.946 kb on + strand, within endA at 851.973 kb on - strand, within endA at 851.974 kb on - strand, within endA at 851.984 kb on - strand, within endA at 852.046 kb on + strand at 852.063 kb on + strand at 852.072 kb on - strand at 852.220 kb on + strand at 852.228 kb on - strand at 852.230 kb on + strand at 852.249 kb on + strand at 852.286 kb on + strand at 852.294 kb on - strand, within SO0834 at 852.392 kb on + strand, within SO0834 at 852.401 kb on - strand, within SO0834 at 852.449 kb on + strand, within SO0834 at 852.538 kb on + strand, within SO0834 at 852.556 kb on - strand, within SO0834 at 852.556 kb on - strand, within SO0834 at 852.556 kb on - strand, within SO0834 at 852.660 kb on + strand, within SO0834 at 852.707 kb on - strand, within SO0834 at 852.798 kb on + strand at 853.001 kb on - strand at 853.001 kb on - strand at 853.040 kb on + strand at 853.070 kb on + strand at 853.085 kb on - strand at 853.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 850,333 + gshB SO0831 0.84 -3.9 850,397 - -2.3 850,462 + -2.9 850,529 + -1.8 850,562 - +0.9 850,622 + -0.7 850,630 - +1.2 850,630 - +0.2 850,632 + -2.8 850,640 - +1.0 850,666 - SO0832 0.12 -0.7 850,763 - SO0832 0.25 +1.4 850,768 + SO0832 0.26 -0.7 850,783 + SO0832 0.28 -0.7 851,007 - SO0832 0.59 +0.3 851,007 - SO0832 0.59 +1.7 851,064 + SO0832 0.67 -0.6 851,162 + SO0832 0.80 -0.0 851,183 - SO0832 0.83 +0.4 851,207 - SO0832 0.86 -1.0 851,218 - SO0832 0.88 +0.5 851,441 + endA SO0833 0.16 -0.9 851,443 - endA SO0833 0.16 +0.6 851,651 + endA SO0833 0.43 -1.9 851,651 + endA SO0833 0.43 +0.6 851,681 - endA SO0833 0.47 -3.3 851,763 - endA SO0833 0.57 +0.5 851,796 - endA SO0833 0.62 -2.6 851,796 - endA SO0833 0.62 +0.9 851,937 - endA SO0833 0.80 -0.0 851,937 - endA SO0833 0.80 +0.2 851,946 + endA SO0833 0.81 -1.5 851,973 - endA SO0833 0.84 +0.0 851,974 - endA SO0833 0.85 -0.7 851,984 - endA SO0833 0.86 +0.6 852,046 + +0.6 852,063 + -1.1 852,072 - +1.0 852,220 + +0.8 852,228 - +1.0 852,230 + +1.1 852,249 + +1.5 852,286 + +1.8 852,294 - SO0834 0.10 +0.9 852,392 + SO0834 0.26 +1.0 852,401 - SO0834 0.27 +0.7 852,449 + SO0834 0.35 +1.5 852,538 + SO0834 0.49 -2.3 852,556 - SO0834 0.52 -0.2 852,556 - SO0834 0.52 +0.4 852,556 - SO0834 0.52 +0.2 852,660 + SO0834 0.69 -0.7 852,707 - SO0834 0.76 +1.3 852,798 + +1.5 853,001 - +1.3 853,001 - +0.2 853,040 + -2.1 853,070 + -3.5 853,085 - +1.2 853,092 - -1.0
Or see this region's nucleotide sequence