Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0815 and SO0816 are separated by 260 nucleotides SO0816 and metR are separated by 56 nucleotides
SO0815: SO0815 - TonB-dependent receptor C-terminal domain protein (NCBI ptt file), at 827,155 to 829,116
SO0815
SO0816: SO0816 - hypothetical protein (NCBI ptt file), at 829,377 to 830,105
SO0816
SO0817: metR - transcriptional activator protein MetR (NCBI ptt file), at 830,162 to 831,067
metR
Position (kb) 829
830
831 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 828.391 kb on - strand, within SO0815 at 828.403 kb on - strand, within SO0815 at 828.419 kb on + strand, within SO0815 at 828.419 kb on + strand, within SO0815 at 828.419 kb on + strand, within SO0815 at 828.479 kb on - strand, within SO0815 at 828.504 kb on + strand, within SO0815 at 828.522 kb on - strand, within SO0815 at 828.554 kb on + strand, within SO0815 at 828.571 kb on + strand, within SO0815 at 828.650 kb on + strand, within SO0815 at 828.657 kb on + strand, within SO0815 at 828.657 kb on + strand, within SO0815 at 828.657 kb on + strand, within SO0815 at 828.836 kb on - strand, within SO0815 at 828.841 kb on - strand, within SO0815 at 828.935 kb on + strand at 829.047 kb on + strand at 829.118 kb on - strand at 829.157 kb on - strand at 829.157 kb on - strand at 829.164 kb on - strand at 829.170 kb on + strand at 829.218 kb on - strand at 829.229 kb on + strand at 829.250 kb on - strand at 829.326 kb on - strand at 829.341 kb on - strand at 829.362 kb on + strand at 829.370 kb on - strand at 829.409 kb on + strand at 829.562 kb on + strand, within SO0816 at 829.562 kb on + strand, within SO0816 at 829.577 kb on + strand, within SO0816 at 829.585 kb on - strand, within SO0816 at 829.585 kb on - strand, within SO0816 at 829.594 kb on + strand, within SO0816 at 829.617 kb on - strand, within SO0816 at 829.648 kb on - strand, within SO0816 at 829.680 kb on - strand, within SO0816 at 829.680 kb on - strand, within SO0816 at 829.772 kb on - strand, within SO0816 at 829.812 kb on + strand, within SO0816 at 829.812 kb on + strand, within SO0816 at 829.812 kb on + strand, within SO0816 at 829.812 kb on + strand, within SO0816 at 829.819 kb on + strand, within SO0816 at 829.820 kb on - strand, within SO0816 at 829.834 kb on + strand, within SO0816 at 830.011 kb on + strand, within SO0816 at 830.044 kb on + strand at 830.050 kb on + strand at 830.055 kb on + strand at 830.055 kb on + strand at 830.063 kb on - strand at 830.063 kb on - strand at 830.063 kb on - strand at 830.183 kb on + strand at 830.191 kb on - strand at 830.273 kb on + strand, within metR at 830.355 kb on - strand, within metR at 830.481 kb on + strand, within metR at 830.600 kb on - strand, within metR at 830.620 kb on + strand, within metR at 830.628 kb on - strand, within metR at 830.662 kb on + strand, within metR at 830.700 kb on + strand, within metR at 830.706 kb on + strand, within metR at 830.743 kb on + strand, within metR at 830.789 kb on - strand, within metR at 830.799 kb on - strand, within metR at 830.835 kb on + strand, within metR at 830.850 kb on - strand, within metR at 830.867 kb on + strand, within metR at 830.902 kb on - strand, within metR at 830.993 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 828,391 - SO0815 0.63 -0.5 828,403 - SO0815 0.64 -0.1 828,419 + SO0815 0.64 -0.7 828,419 + SO0815 0.64 -2.7 828,419 + SO0815 0.64 -2.6 828,479 - SO0815 0.67 -2.4 828,504 + SO0815 0.69 -2.6 828,522 - SO0815 0.70 -0.8 828,554 + SO0815 0.71 -1.8 828,571 + SO0815 0.72 -0.9 828,650 + SO0815 0.76 -4.8 828,657 + SO0815 0.77 -0.1 828,657 + SO0815 0.77 -3.6 828,657 + SO0815 0.77 -3.3 828,836 - SO0815 0.86 -2.6 828,841 - SO0815 0.86 -0.1 828,935 + +1.2 829,047 + -1.2 829,118 - -0.7 829,157 - +0.1 829,157 - +0.4 829,164 - -0.3 829,170 + +0.3 829,218 - -1.4 829,229 + +0.7 829,250 - -0.3 829,326 - -3.1 829,341 - +0.6 829,362 + +0.6 829,370 - -1.1 829,409 + -3.6 829,562 + SO0816 0.25 +1.9 829,562 + SO0816 0.25 -1.4 829,577 + SO0816 0.27 +0.9 829,585 - SO0816 0.29 +0.0 829,585 - SO0816 0.29 -1.4 829,594 + SO0816 0.30 +1.1 829,617 - SO0816 0.33 -4.1 829,648 - SO0816 0.37 -0.9 829,680 - SO0816 0.42 +1.7 829,680 - SO0816 0.42 +2.6 829,772 - SO0816 0.54 +1.9 829,812 + SO0816 0.60 -1.0 829,812 + SO0816 0.60 +0.8 829,812 + SO0816 0.60 +0.5 829,812 + SO0816 0.60 +0.9 829,819 + SO0816 0.61 -0.1 829,820 - SO0816 0.61 +2.3 829,834 + SO0816 0.63 +0.9 830,011 + SO0816 0.87 +4.9 830,044 + +0.6 830,050 + -1.0 830,055 + -2.4 830,055 + -1.3 830,063 - +3.1 830,063 - +2.1 830,063 - -0.3 830,183 + -3.4 830,191 - -3.6 830,273 + metR SO0817 0.12 -3.6 830,355 - metR SO0817 0.21 -2.4 830,481 + metR SO0817 0.35 +0.9 830,600 - metR SO0817 0.48 -3.0 830,620 + metR SO0817 0.51 -0.7 830,628 - metR SO0817 0.51 -4.0 830,662 + metR SO0817 0.55 -4.3 830,700 + metR SO0817 0.59 -2.0 830,706 + metR SO0817 0.60 -5.1 830,743 + metR SO0817 0.64 -1.7 830,789 - metR SO0817 0.69 -3.6 830,799 - metR SO0817 0.70 -2.6 830,835 + metR SO0817 0.74 -3.0 830,850 - metR SO0817 0.76 -4.7 830,867 + metR SO0817 0.78 -1.9 830,902 - metR SO0817 0.82 -4.4 830,993 + -5.3
Or see this region's nucleotide sequence