Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0764 and SO0765 are separated by 128 nucleotides SO0765 and SO0766 are separated by 102 nucleotides SO0766 and SO0766.1 are separated by 165 nucleotides SO0766.1 and SO0767 are separated by 58 nucleotides
SO0764: SO0764 - hypothetical protein (NCBI ptt file), at 778,043 to 778,852
SO0764
SO0765: SO0765 - threonine efflux protein, putative (NCBI ptt file), at 778,981 to 779,685
SO0765
SO0766: SO0766 - transcriptional regulator, AraC/XylS family (NCBI ptt file), at 779,788 to 780,609
SO0766
SO0766.1: SO0766.1 - conserved hypothetical protein (NCBI ptt file), at 780,775 to 781,344
SO0766.1
SO0767: SO0767 - transcriptional regulator, LysR family (NCBI ptt file), at 781,403 to 781,819
SO0767
Position (kb) 779
780
781 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 778.958 kb on + strand at 778.958 kb on + strand at 778.958 kb on + strand at 778.968 kb on + strand at 778.978 kb on + strand at 778.986 kb on - strand at 779.013 kb on - strand at 779.021 kb on - strand at 779.058 kb on + strand, within SO0765 at 779.066 kb on - strand, within SO0765 at 779.108 kb on - strand, within SO0765 at 779.163 kb on + strand, within SO0765 at 779.183 kb on - strand, within SO0765 at 779.206 kb on - strand, within SO0765 at 779.360 kb on + strand, within SO0765 at 779.366 kb on + strand, within SO0765 at 779.386 kb on - strand, within SO0765 at 779.563 kb on + strand, within SO0765 at 779.583 kb on + strand, within SO0765 at 779.583 kb on + strand, within SO0765 at 779.591 kb on - strand, within SO0765 at 779.591 kb on - strand, within SO0765 at 779.663 kb on - strand at 779.663 kb on - strand at 779.696 kb on - strand at 779.734 kb on + strand at 779.734 kb on + strand at 779.774 kb on - strand at 779.903 kb on - strand, within SO0766 at 779.910 kb on + strand, within SO0766 at 779.910 kb on - strand, within SO0766 at 779.931 kb on + strand, within SO0766 at 779.956 kb on + strand, within SO0766 at 779.971 kb on - strand, within SO0766 at 780.035 kb on + strand, within SO0766 at 780.056 kb on - strand, within SO0766 at 780.065 kb on + strand, within SO0766 at 780.087 kb on + strand, within SO0766 at 780.134 kb on - strand, within SO0766 at 780.134 kb on - strand, within SO0766 at 780.258 kb on - strand, within SO0766 at 780.346 kb on - strand, within SO0766 at 780.350 kb on + strand, within SO0766 at 780.358 kb on - strand, within SO0766 at 780.412 kb on - strand, within SO0766 at 780.432 kb on - strand, within SO0766 at 780.443 kb on + strand, within SO0766 at 780.446 kb on - strand, within SO0766 at 780.451 kb on - strand, within SO0766 at 780.488 kb on + strand, within SO0766 at 780.575 kb on - strand at 780.575 kb on - strand at 780.613 kb on + strand at 780.624 kb on - strand at 780.674 kb on + strand at 780.674 kb on + strand at 780.682 kb on - strand at 780.682 kb on - strand at 780.742 kb on - strand at 780.808 kb on - strand at 780.815 kb on - strand at 780.827 kb on - strand at 780.899 kb on - strand, within SO0766.1 at 781.063 kb on - strand, within SO0766.1 at 781.078 kb on - strand, within SO0766.1 at 781.078 kb on - strand, within SO0766.1 at 781.078 kb on - strand, within SO0766.1 at 781.078 kb on - strand, within SO0766.1 at 781.214 kb on + strand, within SO0766.1 at 781.222 kb on + strand, within SO0766.1 at 781.284 kb on + strand, within SO0766.1 at 781.284 kb on + strand at 781.292 kb on - strand at 781.292 kb on - strand at 781.295 kb on + strand at 781.325 kb on - strand at 781.328 kb on + strand at 781.336 kb on - strand at 781.378 kb on + strand at 781.523 kb on + strand, within SO0767 at 781.531 kb on - strand, within SO0767 at 781.533 kb on + strand, within SO0767
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 778,958 + +2.7 778,958 + +1.7 778,958 + +1.9 778,968 + +1.5 778,978 + +3.2 778,986 - +1.0 779,013 - +0.4 779,021 - -0.7 779,058 + SO0765 0.11 +0.8 779,066 - SO0765 0.12 -0.5 779,108 - SO0765 0.18 -3.0 779,163 + SO0765 0.26 -0.5 779,183 - SO0765 0.29 -3.0 779,206 - SO0765 0.32 +0.1 779,360 + SO0765 0.54 -0.7 779,366 + SO0765 0.55 -1.7 779,386 - SO0765 0.57 -1.0 779,563 + SO0765 0.83 -2.9 779,583 + SO0765 0.85 -1.0 779,583 + SO0765 0.85 +0.7 779,591 - SO0765 0.87 +0.6 779,591 - SO0765 0.87 -0.1 779,663 - +0.1 779,663 - -0.3 779,696 - +0.3 779,734 + -3.3 779,734 + +0.3 779,774 - +2.1 779,903 - SO0766 0.14 -0.5 779,910 + SO0766 0.15 -3.7 779,910 - SO0766 0.15 +2.0 779,931 + SO0766 0.17 -1.3 779,956 + SO0766 0.20 -1.0 779,971 - SO0766 0.22 +0.7 780,035 + SO0766 0.30 -0.7 780,056 - SO0766 0.33 +1.4 780,065 + SO0766 0.34 +0.5 780,087 + SO0766 0.36 -0.9 780,134 - SO0766 0.42 -1.6 780,134 - SO0766 0.42 -0.6 780,258 - SO0766 0.57 -1.4 780,346 - SO0766 0.68 -0.9 780,350 + SO0766 0.68 +0.8 780,358 - SO0766 0.69 +0.5 780,412 - SO0766 0.76 -2.4 780,432 - SO0766 0.78 +0.1 780,443 + SO0766 0.80 -1.1 780,446 - SO0766 0.80 +1.9 780,451 - SO0766 0.81 -0.7 780,488 + SO0766 0.85 -0.1 780,575 - -2.9 780,575 - -2.3 780,613 + +2.0 780,624 - -1.5 780,674 + +0.1 780,674 + +0.9 780,682 - +0.2 780,682 - +1.9 780,742 - +0.9 780,808 - -1.5 780,815 - -0.3 780,827 - -1.1 780,899 - SO0766.1 0.22 -0.3 781,063 - SO0766.1 0.51 -1.6 781,078 - SO0766.1 0.53 +0.0 781,078 - SO0766.1 0.53 +0.1 781,078 - SO0766.1 0.53 -2.8 781,078 - SO0766.1 0.53 -0.2 781,214 + SO0766.1 0.77 +0.9 781,222 + SO0766.1 0.78 -2.4 781,284 + SO0766.1 0.89 +0.4 781,284 + -0.7 781,292 - -2.1 781,292 - +0.1 781,295 + +1.0 781,325 - -0.3 781,328 + -1.3 781,336 - -0.6 781,378 + +0.1 781,523 + SO0767 0.29 -1.1 781,531 - SO0767 0.31 -0.1 781,533 + SO0767 0.31 +1.0
Or see this region's nucleotide sequence