Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0762 and SO0763 are separated by 376 nucleotides SO0763 and SO0764 overlap by 14 nucleotides SO0764 and SO0765 are separated by 128 nucleotides SO0765 and SO0766 are separated by 102 nucleotides
SO0762: SO0762 - isochorismatase family protein (NCBI ptt file), at 776,916 to 777,458
SO0762
SO0763: SO0763 - hypothetical protein (NCBI ptt file), at 777,835 to 778,056
SO0763
SO0764: SO0764 - hypothetical protein (NCBI ptt file), at 778,043 to 778,852
SO0764
SO0765: SO0765 - threonine efflux protein, putative (NCBI ptt file), at 778,981 to 779,685
SO0765
SO0766: SO0766 - transcriptional regulator, AraC/XylS family (NCBI ptt file), at 779,788 to 780,609
SO0766
Position (kb)
778
779 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 777.053 kb on + strand, within SO0762 at 777.063 kb on + strand, within SO0762 at 777.156 kb on + strand, within SO0762 at 777.174 kb on - strand, within SO0762 at 777.319 kb on + strand, within SO0762 at 777.369 kb on + strand, within SO0762 at 777.384 kb on - strand, within SO0762 at 777.408 kb on + strand at 777.468 kb on + strand at 777.497 kb on + strand at 777.497 kb on + strand at 777.497 kb on + strand at 777.497 kb on + strand at 777.505 kb on - strand at 777.505 kb on - strand at 777.505 kb on - strand at 777.505 kb on - strand at 777.517 kb on - strand at 777.533 kb on - strand at 777.540 kb on + strand at 777.556 kb on + strand at 777.556 kb on + strand at 777.556 kb on + strand at 777.617 kb on - strand at 777.619 kb on - strand at 777.667 kb on - strand at 777.927 kb on - strand, within SO0763 at 777.927 kb on - strand, within SO0763 at 777.963 kb on + strand, within SO0763 at 777.963 kb on + strand, within SO0763 at 777.963 kb on + strand, within SO0763 at 777.971 kb on - strand, within SO0763 at 777.971 kb on - strand, within SO0763 at 777.971 kb on - strand, within SO0763 at 777.983 kb on - strand, within SO0763 at 778.021 kb on + strand, within SO0763 at 778.022 kb on + strand, within SO0763 at 778.030 kb on - strand, within SO0763 at 778.030 kb on - strand, within SO0763 at 778.050 kb on + strand at 778.058 kb on - strand at 778.106 kb on + strand at 778.120 kb on + strand at 778.140 kb on - strand, within SO0764 at 778.158 kb on - strand, within SO0764 at 778.233 kb on + strand, within SO0764 at 778.268 kb on - strand, within SO0764 at 778.275 kb on + strand, within SO0764 at 778.342 kb on + strand, within SO0764 at 778.342 kb on + strand, within SO0764 at 778.350 kb on - strand, within SO0764 at 778.423 kb on + strand, within SO0764 at 778.423 kb on + strand, within SO0764 at 778.515 kb on - strand, within SO0764 at 778.533 kb on + strand, within SO0764 at 778.533 kb on + strand, within SO0764 at 778.541 kb on - strand, within SO0764 at 778.541 kb on - strand, within SO0764 at 778.541 kb on - strand, within SO0764 at 778.541 kb on - strand, within SO0764 at 778.686 kb on - strand, within SO0764 at 778.958 kb on + strand at 778.958 kb on + strand at 778.958 kb on + strand at 778.968 kb on + strand at 778.978 kb on + strand at 778.986 kb on - strand at 779.013 kb on - strand at 779.021 kb on - strand at 779.058 kb on + strand, within SO0765 at 779.066 kb on - strand, within SO0765 at 779.108 kb on - strand, within SO0765 at 779.163 kb on + strand, within SO0765 at 779.183 kb on - strand, within SO0765 at 779.206 kb on - strand, within SO0765 at 779.360 kb on + strand, within SO0765 at 779.366 kb on + strand, within SO0765 at 779.386 kb on - strand, within SO0765 at 779.563 kb on + strand, within SO0765 at 779.583 kb on + strand, within SO0765 at 779.583 kb on + strand, within SO0765 at 779.591 kb on - strand, within SO0765 at 779.591 kb on - strand, within SO0765 at 779.663 kb on - strand at 779.663 kb on - strand at 779.696 kb on - strand at 779.734 kb on + strand at 779.734 kb on + strand at 779.774 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 777,053 + SO0762 0.25 -1.7 777,063 + SO0762 0.27 -1.1 777,156 + SO0762 0.44 -0.1 777,174 - SO0762 0.48 -0.2 777,319 + SO0762 0.74 -0.1 777,369 + SO0762 0.83 +1.2 777,384 - SO0762 0.86 +0.3 777,408 + +1.3 777,468 + +1.9 777,497 + -2.3 777,497 + +1.1 777,497 + +1.1 777,497 + -2.7 777,505 - -1.4 777,505 - -1.1 777,505 - +0.2 777,505 - +0.5 777,517 - +1.4 777,533 - -3.0 777,540 + -2.7 777,556 + +1.6 777,556 + +2.1 777,556 + -0.4 777,617 - +0.6 777,619 - -0.7 777,667 - +0.5 777,927 - SO0763 0.41 +0.0 777,927 - SO0763 0.41 +1.2 777,963 + SO0763 0.58 +1.6 777,963 + SO0763 0.58 +0.9 777,963 + SO0763 0.58 +1.1 777,971 - SO0763 0.61 -2.7 777,971 - SO0763 0.61 -0.5 777,971 - SO0763 0.61 +0.9 777,983 - SO0763 0.67 +1.5 778,021 + SO0763 0.84 +0.7 778,022 + SO0763 0.84 -0.6 778,030 - SO0763 0.88 +1.1 778,030 - SO0763 0.88 +1.2 778,050 + +3.3 778,058 - -1.9 778,106 + -1.8 778,120 + -0.1 778,140 - SO0764 0.12 +0.2 778,158 - SO0764 0.14 -0.9 778,233 + SO0764 0.23 -0.1 778,268 - SO0764 0.28 -2.8 778,275 + SO0764 0.29 +0.1 778,342 + SO0764 0.37 -0.7 778,342 + SO0764 0.37 -0.5 778,350 - SO0764 0.38 +0.7 778,423 + SO0764 0.47 +0.0 778,423 + SO0764 0.47 -1.2 778,515 - SO0764 0.58 -0.5 778,533 + SO0764 0.60 +1.2 778,533 + SO0764 0.60 -0.5 778,541 - SO0764 0.61 +0.4 778,541 - SO0764 0.61 +0.5 778,541 - SO0764 0.61 -1.3 778,541 - SO0764 0.61 -0.0 778,686 - SO0764 0.79 -1.7 778,958 + +2.7 778,958 + +1.7 778,958 + +1.9 778,968 + +1.5 778,978 + +3.2 778,986 - +1.0 779,013 - +0.4 779,021 - -0.7 779,058 + SO0765 0.11 +0.8 779,066 - SO0765 0.12 -0.5 779,108 - SO0765 0.18 -3.0 779,163 + SO0765 0.26 -0.5 779,183 - SO0765 0.29 -3.0 779,206 - SO0765 0.32 +0.1 779,360 + SO0765 0.54 -0.7 779,366 + SO0765 0.55 -1.7 779,386 - SO0765 0.57 -1.0 779,563 + SO0765 0.83 -2.9 779,583 + SO0765 0.85 -1.0 779,583 + SO0765 0.85 +0.7 779,591 - SO0765 0.87 +0.6 779,591 - SO0765 0.87 -0.1 779,663 - +0.1 779,663 - -0.3 779,696 - +0.3 779,734 + -3.3 779,734 + +0.3 779,774 - +2.1
Or see this region's nucleotide sequence