Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sspB and pabA are separated by 154 nucleotides pabA and SO0614 are separated by 172 nucleotides SO0614 and SO0615 are separated by 187 nucleotides
SO0612: sspB - stringent starvation protein b (NCBI ptt file), at 637,024 to 637,461
sspB
SO0613: pabA - para-aminobenzoate synthase glutamine amidotransferase, component II (NCBI ptt file), at 637,616 to 638,191
pabA
SO0614: SO0614 - dipeptidyl peptidase IV, putative (NCBI ptt file), at 638,364 to 640,844
SO0614
SO0615: SO0615 - hypothetical protein (NCBI ptt file), at 641,032 to 642,177
SO0615
Position (kb) 638
639
640
641 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.587 kb on + strand at 637.595 kb on - strand at 637.659 kb on - strand at 637.737 kb on + strand, within pabA at 637.742 kb on + strand, within pabA at 637.772 kb on - strand, within pabA at 637.857 kb on - strand, within pabA at 638.009 kb on + strand, within pabA at 638.056 kb on + strand, within pabA at 638.088 kb on + strand, within pabA at 638.090 kb on + strand, within pabA at 638.152 kb on - strand at 638.244 kb on + strand at 638.315 kb on - strand at 638.321 kb on + strand at 638.321 kb on + strand at 638.329 kb on - strand at 638.329 kb on - strand at 638.366 kb on - strand at 638.422 kb on - strand at 638.447 kb on + strand at 638.472 kb on + strand at 638.674 kb on + strand, within SO0614 at 638.682 kb on - strand, within SO0614 at 638.737 kb on - strand, within SO0614 at 638.750 kb on + strand, within SO0614 at 638.758 kb on - strand, within SO0614 at 638.800 kb on + strand, within SO0614 at 638.908 kb on + strand, within SO0614 at 639.264 kb on + strand, within SO0614 at 639.304 kb on + strand, within SO0614 at 639.336 kb on - strand, within SO0614 at 639.421 kb on - strand, within SO0614 at 639.437 kb on + strand, within SO0614 at 639.442 kb on - strand, within SO0614 at 639.484 kb on - strand, within SO0614 at 639.486 kb on - strand, within SO0614 at 639.533 kb on + strand, within SO0614 at 639.587 kb on + strand, within SO0614 at 639.593 kb on + strand, within SO0614 at 639.696 kb on - strand, within SO0614 at 639.834 kb on - strand, within SO0614 at 639.947 kb on + strand, within SO0614 at 639.955 kb on - strand, within SO0614 at 640.006 kb on + strand, within SO0614 at 640.039 kb on + strand, within SO0614 at 640.174 kb on + strand, within SO0614 at 640.174 kb on + strand, within SO0614 at 640.237 kb on + strand, within SO0614 at 640.263 kb on + strand, within SO0614 at 640.270 kb on + strand, within SO0614 at 640.308 kb on + strand, within SO0614 at 640.432 kb on - strand, within SO0614 at 640.462 kb on + strand, within SO0614 at 640.539 kb on - strand, within SO0614 at 640.639 kb on + strand at 640.659 kb on + strand at 640.707 kb on - strand at 640.709 kb on + strand at 640.758 kb on + strand at 640.866 kb on - strand at 640.870 kb on + strand at 640.925 kb on + strand at 640.951 kb on - strand at 641.032 kb on - strand at 641.055 kb on + strand at 641.110 kb on - strand at 641.129 kb on + strand at 641.137 kb on - strand at 641.137 kb on - strand at 641.137 kb on - strand at 641.268 kb on + strand, within SO0615 at 641.280 kb on + strand, within SO0615 at 641.401 kb on - strand, within SO0615 at 641.408 kb on - strand, within SO0615 at 641.415 kb on - strand, within SO0615 at 641.462 kb on - strand, within SO0615 at 641.491 kb on - strand, within SO0615 at 641.492 kb on + strand, within SO0615 at 641.492 kb on + strand, within SO0615 at 641.492 kb on + strand, within SO0615 at 641.613 kb on + strand, within SO0615 at 641.613 kb on + strand, within SO0615 at 641.621 kb on - strand, within SO0615 at 641.621 kb on - strand, within SO0615 at 641.703 kb on - strand, within SO0615 at 641.706 kb on + strand, within SO0615 at 641.749 kb on - strand, within SO0615 at 641.764 kb on - strand, within SO0615 at 641.830 kb on + strand, within SO0615
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 637,587 + +0.4 637,587 + +1.3 637,587 + +2.4 637,587 + -0.9 637,587 + -0.3 637,595 - -0.9 637,659 - +1.0 637,737 + pabA SO0613 0.21 +1.4 637,742 + pabA SO0613 0.22 +3.2 637,772 - pabA SO0613 0.27 -1.3 637,857 - pabA SO0613 0.42 -1.1 638,009 + pabA SO0613 0.68 +1.8 638,056 + pabA SO0613 0.76 +0.6 638,088 + pabA SO0613 0.82 -1.3 638,090 + pabA SO0613 0.82 +0.4 638,152 - -0.0 638,244 + +0.3 638,315 - -0.9 638,321 + +0.3 638,321 + +0.5 638,329 - -0.2 638,329 - -1.0 638,366 - -0.5 638,422 - -0.7 638,447 + +0.5 638,472 + +0.7 638,674 + SO0614 0.12 +1.4 638,682 - SO0614 0.13 +2.8 638,737 - SO0614 0.15 +0.3 638,750 + SO0614 0.16 +1.4 638,758 - SO0614 0.16 -2.0 638,800 + SO0614 0.18 -0.1 638,908 + SO0614 0.22 +0.1 639,264 + SO0614 0.36 -0.4 639,304 + SO0614 0.38 -0.0 639,336 - SO0614 0.39 +0.3 639,421 - SO0614 0.43 -0.5 639,437 + SO0614 0.43 -1.6 639,442 - SO0614 0.43 +1.2 639,484 - SO0614 0.45 +0.6 639,486 - SO0614 0.45 -1.9 639,533 + SO0614 0.47 -0.1 639,587 + SO0614 0.49 +0.2 639,593 + SO0614 0.50 +1.1 639,696 - SO0614 0.54 +1.4 639,834 - SO0614 0.59 -2.2 639,947 + SO0614 0.64 -0.6 639,955 - SO0614 0.64 +0.4 640,006 + SO0614 0.66 -0.6 640,039 + SO0614 0.68 +0.1 640,174 + SO0614 0.73 +0.6 640,174 + SO0614 0.73 -1.4 640,237 + SO0614 0.75 -1.9 640,263 + SO0614 0.77 -0.0 640,270 + SO0614 0.77 -2.4 640,308 + SO0614 0.78 +0.6 640,432 - SO0614 0.83 -1.6 640,462 + SO0614 0.85 -1.7 640,539 - SO0614 0.88 -1.6 640,639 + -2.8 640,659 + -2.1 640,707 - +2.8 640,709 + -1.1 640,758 + -0.5 640,866 - -1.8 640,870 + +0.9 640,925 + +0.8 640,951 - -0.4 641,032 - -3.8 641,055 + -1.1 641,110 - +0.3 641,129 + -3.3 641,137 - -0.0 641,137 - -1.9 641,137 - -0.9 641,268 + SO0615 0.21 -1.5 641,280 + SO0615 0.22 -0.3 641,401 - SO0615 0.32 -0.8 641,408 - SO0615 0.33 +0.2 641,415 - SO0615 0.33 -1.3 641,462 - SO0615 0.38 -0.9 641,491 - SO0615 0.40 -1.0 641,492 + SO0615 0.40 -1.8 641,492 + SO0615 0.40 +0.2 641,492 + SO0615 0.40 +1.0 641,613 + SO0615 0.51 -0.0 641,613 + SO0615 0.51 -1.5 641,621 - SO0615 0.51 -1.8 641,621 - SO0615 0.51 -0.1 641,703 - SO0615 0.59 -3.5 641,706 + SO0615 0.59 +0.1 641,749 - SO0615 0.63 -1.8 641,764 - SO0615 0.64 -0.7 641,830 + SO0615 0.70 -0.7
Or see this region's nucleotide sequence