Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjeF and SO0599 are separated by 80 nucleotides SO0599 and amiB are separated by 8 nucleotides amiB and mutL are separated by 13 nucleotides
SO0598: yjeF - yjeF protein (NCBI ptt file), at 623,398 to 624,897
yjeF
SO0599: SO0599 - conserved hypothetical protein TIGR00150 (NCBI ptt file), at 624,978 to 625,436
SO0599
SO0600: amiB - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 625,445 to 626,836
amiB
SO0601: mutL - DNA mismatch repair protein MutL (NCBI ptt file), at 626,850 to 628,745
mutL
Position (kb) 625
626
627 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 624.542 kb on + strand, within yjeF at 624.565 kb on - strand, within yjeF at 624.567 kb on + strand, within yjeF at 624.575 kb on - strand, within yjeF at 624.619 kb on - strand, within yjeF at 624.629 kb on - strand, within yjeF at 624.683 kb on + strand, within yjeF at 624.790 kb on + strand at 624.790 kb on + strand at 624.790 kb on + strand at 624.790 kb on + strand at 624.790 kb on + strand at 624.798 kb on - strand at 624.820 kb on + strand at 624.891 kb on + strand at 624.906 kb on + strand at 625.518 kb on + strand at 625.543 kb on + strand at 625.865 kb on - strand, within amiB at 625.886 kb on - strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.431 kb on + strand, within amiB at 626.663 kb on + strand, within amiB at 626.898 kb on + strand at 626.920 kb on + strand at 626.958 kb on - strand at 626.964 kb on - strand at 626.998 kb on + strand at 626.998 kb on + strand at 627.062 kb on - strand, within mutL at 627.102 kb on - strand, within mutL at 627.104 kb on + strand, within mutL at 627.112 kb on - strand, within mutL at 627.190 kb on + strand, within mutL at 627.190 kb on + strand, within mutL at 627.198 kb on - strand, within mutL at 627.200 kb on + strand, within mutL at 627.280 kb on + strand, within mutL at 627.370 kb on + strand, within mutL at 627.384 kb on + strand, within mutL at 627.546 kb on + strand, within mutL at 627.588 kb on + strand, within mutL at 627.588 kb on + strand, within mutL at 627.588 kb on - strand, within mutL at 627.708 kb on - strand, within mutL
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 624,542 + yjeF SO0598 0.76 -1.0 624,565 - yjeF SO0598 0.78 -1.3 624,567 + yjeF SO0598 0.78 +1.4 624,575 - yjeF SO0598 0.78 +0.7 624,619 - yjeF SO0598 0.81 -1.0 624,629 - yjeF SO0598 0.82 -0.6 624,683 + yjeF SO0598 0.86 +1.8 624,790 + +0.4 624,790 + +0.7 624,790 + -1.5 624,790 + +1.4 624,790 + +2.0 624,798 - -0.4 624,820 + -0.4 624,891 + -0.7 624,906 + +1.7 625,518 + -3.7 625,543 + -0.5 625,865 - amiB SO0600 0.30 -1.8 625,886 - amiB SO0600 0.32 -0.1 626,364 + amiB SO0600 0.66 -3.7 626,364 + amiB SO0600 0.66 +3.6 626,364 + amiB SO0600 0.66 +5.3 626,364 + amiB SO0600 0.66 +0.9 626,431 + amiB SO0600 0.71 +0.6 626,663 + amiB SO0600 0.88 +1.1 626,898 + -0.2 626,920 + -0.6 626,958 - -1.4 626,964 - -2.2 626,998 + -0.2 626,998 + +0.6 627,062 - mutL SO0601 0.11 -0.0 627,102 - mutL SO0601 0.13 -0.4 627,104 + mutL SO0601 0.13 +1.2 627,112 - mutL SO0601 0.14 -1.9 627,190 + mutL SO0601 0.18 -1.8 627,190 + mutL SO0601 0.18 +1.1 627,198 - mutL SO0601 0.18 -2.6 627,200 + mutL SO0601 0.18 -3.1 627,280 + mutL SO0601 0.23 -3.1 627,370 + mutL SO0601 0.27 -1.7 627,384 + mutL SO0601 0.28 +1.7 627,546 + mutL SO0601 0.37 -1.0 627,588 + mutL SO0601 0.39 +1.1 627,588 + mutL SO0601 0.39 -2.0 627,588 - mutL SO0601 0.39 +0.3 627,708 - mutL SO0601 0.45 -2.7
Or see this region's nucleotide sequence