Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO_0419 and SO0420.2 are separated by 44 nucleotides SO0420.2 and SO0420 are separated by 254 nucleotides SO0420 and ampD overlap by 54 nucleotides ampD and ampE are separated by 269 nucleotides
SO_0419: SO_0419 - no description, at 434,288 to 434,911
_0419
SO0420.2: SO0420.2 - hypothetical nicotinate-nucleotide pyrophosphorylase (NCBI ptt file), at 434,956 to 435,168
SO0420.2
SO0420: SO0420 - hypothetical protein (NCBI ptt file), at 435,423 to 435,515
SO0420
SO0421: ampD - AmpD protein (NCBI ptt file), at 435,462 to 436,007
ampD
SO0422: ampE - AmpE protein (NCBI ptt file), at 436,277 to 437,128
ampE
Position (kb) 435
436
437 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 434.728 kb on + strand, within SO_0419 at 434.731 kb on - strand, within SO_0419 at 434.939 kb on - strand at 434.974 kb on + strand at 434.987 kb on - strand, within SO0420.2 at 435.235 kb on - strand at 435.256 kb on - strand at 435.266 kb on - strand at 435.278 kb on + strand at 435.278 kb on + strand at 435.278 kb on + strand at 435.286 kb on - strand at 435.393 kb on + strand at 435.431 kb on - strand at 435.431 kb on - strand at 435.514 kb on + strand at 435.544 kb on + strand, within ampD at 435.546 kb on - strand, within ampD at 435.591 kb on - strand, within ampD at 435.601 kb on - strand, within ampD at 435.601 kb on - strand, within ampD at 435.601 kb on - strand, within ampD at 435.646 kb on + strand, within ampD at 435.664 kb on + strand, within ampD at 435.724 kb on - strand, within ampD at 435.731 kb on + strand, within ampD at 435.744 kb on - strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.827 kb on + strand, within ampD at 435.835 kb on - strand, within ampD at 435.835 kb on - strand, within ampD at 435.897 kb on - strand, within ampD at 435.897 kb on - strand, within ampD at 435.923 kb on + strand, within ampD at 435.923 kb on + strand, within ampD at 436.017 kb on - strand at 436.026 kb on + strand at 436.097 kb on - strand at 436.097 kb on - strand at 436.195 kb on + strand at 436.195 kb on + strand at 436.276 kb on - strand at 436.418 kb on - strand, within ampE at 436.488 kb on - strand, within ampE at 436.490 kb on + strand, within ampE at 436.498 kb on - strand, within ampE at 436.499 kb on - strand, within ampE at 436.503 kb on + strand, within ampE at 436.595 kb on + strand, within ampE at 436.603 kb on - strand, within ampE at 436.603 kb on - strand, within ampE at 436.609 kb on + strand, within ampE at 436.617 kb on - strand, within ampE at 436.617 kb on - strand, within ampE at 436.622 kb on + strand, within ampE at 436.719 kb on - strand, within ampE at 436.768 kb on + strand, within ampE at 436.768 kb on + strand, within ampE at 436.776 kb on - strand, within ampE at 436.776 kb on - strand, within ampE at 436.776 kb on - strand, within ampE at 436.776 kb on - strand, within ampE at 436.904 kb on - strand, within ampE at 436.911 kb on - strand, within ampE at 436.911 kb on - strand, within ampE at 436.915 kb on + strand, within ampE at 436.915 kb on + strand, within ampE at 436.915 kb on + strand, within ampE at 436.946 kb on - strand, within ampE at 436.974 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 437.004 kb on - strand, within ampE at 437.004 kb on - strand, within ampE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 434,728 + SO_0419 0.71 -1.5 434,731 - SO_0419 0.71 -1.7 434,939 - -2.3 434,974 + -1.5 434,987 - SO0420.2 0.15 -0.9 435,235 - -0.1 435,256 - -1.5 435,266 - +2.1 435,278 + -0.2 435,278 + +0.1 435,278 + +0.2 435,286 - -1.7 435,393 + -1.5 435,431 - -0.2 435,431 - -1.2 435,514 + -2.5 435,544 + ampD SO0421 0.15 -0.8 435,546 - ampD SO0421 0.15 -3.9 435,591 - ampD SO0421 0.24 -4.1 435,601 - ampD SO0421 0.25 -2.4 435,601 - ampD SO0421 0.25 -1.5 435,601 - ampD SO0421 0.25 -5.2 435,646 + ampD SO0421 0.34 -3.6 435,664 + ampD SO0421 0.37 -3.8 435,724 - ampD SO0421 0.48 -3.8 435,731 + ampD SO0421 0.49 -3.3 435,744 - ampD SO0421 0.52 -3.1 435,827 + ampD SO0421 0.67 -5.1 435,827 + ampD SO0421 0.67 -1.7 435,827 + ampD SO0421 0.67 -0.8 435,827 + ampD SO0421 0.67 -1.5 435,827 + ampD SO0421 0.67 -1.2 435,827 + ampD SO0421 0.67 +0.1 435,827 + ampD SO0421 0.67 -3.1 435,835 - ampD SO0421 0.68 -2.3 435,835 - ampD SO0421 0.68 -3.3 435,897 - ampD SO0421 0.80 -0.5 435,897 - ampD SO0421 0.80 -3.1 435,923 + ampD SO0421 0.84 -2.6 435,923 + ampD SO0421 0.84 -4.4 436,017 - -0.0 436,026 + +0.2 436,097 - +0.3 436,097 - -0.7 436,195 + -0.7 436,195 + -0.7 436,276 - +0.3 436,418 - ampE SO0422 0.17 +0.1 436,488 - ampE SO0422 0.25 -0.0 436,490 + ampE SO0422 0.25 -0.9 436,498 - ampE SO0422 0.26 -1.5 436,499 - ampE SO0422 0.26 -0.5 436,503 + ampE SO0422 0.27 -1.7 436,595 + ampE SO0422 0.37 -0.5 436,603 - ampE SO0422 0.38 +3.1 436,603 - ampE SO0422 0.38 +3.4 436,609 + ampE SO0422 0.39 -0.5 436,617 - ampE SO0422 0.40 -2.3 436,617 - ampE SO0422 0.40 +0.3 436,622 + ampE SO0422 0.40 -2.2 436,719 - ampE SO0422 0.52 -1.7 436,768 + ampE SO0422 0.58 +0.1 436,768 + ampE SO0422 0.58 +0.1 436,776 - ampE SO0422 0.59 -0.2 436,776 - ampE SO0422 0.59 +0.6 436,776 - ampE SO0422 0.59 -0.3 436,776 - ampE SO0422 0.59 -1.9 436,904 - ampE SO0422 0.74 +0.5 436,911 - ampE SO0422 0.74 +2.6 436,911 - ampE SO0422 0.74 +1.1 436,915 + ampE SO0422 0.75 -0.8 436,915 + ampE SO0422 0.75 -0.6 436,915 + ampE SO0422 0.75 +0.6 436,946 - ampE SO0422 0.79 -0.3 436,974 + ampE SO0422 0.82 -0.4 436,996 + ampE SO0422 0.84 +0.6 436,996 + ampE SO0422 0.84 -0.3 436,996 + ampE SO0422 0.84 -3.1 436,996 + ampE SO0422 0.84 +0.8 437,004 - ampE SO0422 0.85 -0.4 437,004 - ampE SO0422 0.85 -0.2
Or see this region's nucleotide sequence