Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0415 and SO0416 are separated by 251 nucleotides SO0416 and SO0417 are separated by 73 nucleotides SO0417 and SO0418 are separated by 106 nucleotides SO0418 and SO_0419 are separated by 275 nucleotides
SO0415: SO0415 - type IV pilus biogenesis protein PilC (NCBI ptt file), at 430,085 to 431,356
SO0415
SO0416: SO0416 - type IV pilus biogenesis protein PilB (NCBI ptt file), at 431,608 to 433,317
SO0416
SO0417: SO0417 - pilin, putative (NCBI ptt file), at 433,391 to 433,807
SO0417
SO0418: SO0418 - hypothetical protein (NCBI ptt file), at 433,914 to 434,012
SO0418
SO_0419: SO_0419 - no description, at 434,288 to 434,911
_0419
Position (kb) 431
432
433
434 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 430.745 kb on + strand, within SO0415 at 430.745 kb on + strand, within SO0415 at 430.788 kb on + strand, within SO0415 at 430.841 kb on + strand, within SO0415 at 430.867 kb on + strand, within SO0415 at 430.896 kb on - strand, within SO0415 at 430.945 kb on + strand, within SO0415 at 431.184 kb on - strand, within SO0415 at 431.184 kb on - strand, within SO0415 at 431.261 kb on + strand at 431.431 kb on - strand at 431.570 kb on + strand at 431.570 kb on + strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.578 kb on - strand at 431.703 kb on + strand at 431.703 kb on - strand at 431.811 kb on + strand, within SO0416 at 431.837 kb on + strand, within SO0416 at 431.837 kb on + strand, within SO0416 at 431.837 kb on + strand, within SO0416 at 431.956 kb on - strand, within SO0416 at 432.028 kb on + strand, within SO0416 at 432.030 kb on - strand, within SO0416 at 432.036 kb on - strand, within SO0416 at 432.166 kb on + strand, within SO0416 at 432.166 kb on + strand, within SO0416 at 432.230 kb on + strand, within SO0416 at 432.230 kb on + strand, within SO0416 at 432.230 kb on + strand, within SO0416 at 432.238 kb on - strand, within SO0416 at 432.239 kb on - strand, within SO0416 at 432.289 kb on + strand, within SO0416 at 432.299 kb on + strand, within SO0416 at 432.299 kb on + strand, within SO0416 at 432.322 kb on - strand, within SO0416 at 432.334 kb on - strand, within SO0416 at 432.334 kb on - strand, within SO0416 at 432.359 kb on + strand, within SO0416 at 432.488 kb on + strand, within SO0416 at 432.503 kb on - strand, within SO0416 at 432.525 kb on + strand, within SO0416 at 432.538 kb on - strand, within SO0416 at 432.595 kb on - strand, within SO0416 at 432.595 kb on - strand, within SO0416 at 432.612 kb on + strand, within SO0416 at 432.713 kb on - strand, within SO0416 at 432.778 kb on + strand, within SO0416 at 432.812 kb on - strand, within SO0416 at 432.846 kb on + strand, within SO0416 at 432.967 kb on - strand, within SO0416 at 432.977 kb on - strand, within SO0416 at 432.993 kb on + strand, within SO0416 at 432.993 kb on + strand, within SO0416 at 433.001 kb on - strand, within SO0416 at 433.080 kb on + strand, within SO0416 at 433.098 kb on + strand, within SO0416 at 433.112 kb on + strand, within SO0416 at 433.140 kb on - strand, within SO0416 at 433.152 kb on + strand at 433.183 kb on - strand at 433.185 kb on + strand at 433.193 kb on - strand at 433.193 kb on - strand at 433.291 kb on + strand at 433.291 kb on + strand at 433.296 kb on + strand at 433.305 kb on - strand at 433.343 kb on + strand at 433.361 kb on - strand at 433.361 kb on - strand at 433.437 kb on - strand, within SO0417 at 433.444 kb on - strand, within SO0417 at 433.450 kb on + strand, within SO0417 at 433.455 kb on + strand, within SO0417 at 433.525 kb on - strand, within SO0417 at 433.546 kb on + strand, within SO0417 at 433.546 kb on + strand, within SO0417 at 433.554 kb on - strand, within SO0417 at 433.604 kb on + strand, within SO0417 at 433.634 kb on - strand at 433.815 kb on + strand at 433.815 kb on + strand at 433.815 kb on + strand at 433.815 kb on + strand at 433.817 kb on + strand at 433.823 kb on - strand at 433.825 kb on - strand at 434.106 kb on + strand at 434.106 kb on + strand at 434.136 kb on + strand at 434.169 kb on + strand at 434.311 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 430,745 + SO0415 0.52 -3.1 430,745 + SO0415 0.52 -0.3 430,788 + SO0415 0.55 +0.8 430,841 + SO0415 0.59 +1.5 430,867 + SO0415 0.61 -2.6 430,896 - SO0415 0.64 +0.8 430,945 + SO0415 0.68 -0.1 431,184 - SO0415 0.86 +0.0 431,184 - SO0415 0.86 +2.3 431,261 + -0.7 431,431 - -0.9 431,570 + +0.5 431,570 + +0.1 431,578 - +0.8 431,578 - -2.0 431,578 - +0.4 431,578 - +0.7 431,578 - +0.0 431,578 - +2.3 431,703 + +0.5 431,703 - +0.8 431,811 + SO0416 0.12 +0.6 431,837 + SO0416 0.13 -2.1 431,837 + SO0416 0.13 +1.1 431,837 + SO0416 0.13 +0.2 431,956 - SO0416 0.20 +0.5 432,028 + SO0416 0.25 +0.3 432,030 - SO0416 0.25 +0.1 432,036 - SO0416 0.25 +0.7 432,166 + SO0416 0.33 +1.2 432,166 + SO0416 0.33 -1.6 432,230 + SO0416 0.36 +1.2 432,230 + SO0416 0.36 +0.8 432,230 + SO0416 0.36 +0.1 432,238 - SO0416 0.37 -0.2 432,239 - SO0416 0.37 +0.5 432,289 + SO0416 0.40 -1.9 432,299 + SO0416 0.40 +2.7 432,299 + SO0416 0.40 +1.0 432,322 - SO0416 0.42 +1.3 432,334 - SO0416 0.42 +2.1 432,334 - SO0416 0.42 -0.1 432,359 + SO0416 0.44 +1.6 432,488 + SO0416 0.51 -1.7 432,503 - SO0416 0.52 +1.1 432,525 + SO0416 0.54 +0.2 432,538 - SO0416 0.54 +0.1 432,595 - SO0416 0.58 -0.0 432,595 - SO0416 0.58 +0.3 432,612 + SO0416 0.59 -0.8 432,713 - SO0416 0.65 +2.3 432,778 + SO0416 0.68 +1.1 432,812 - SO0416 0.70 +1.1 432,846 + SO0416 0.72 -1.2 432,967 - SO0416 0.79 +1.3 432,977 - SO0416 0.80 -1.6 432,993 + SO0416 0.81 +0.1 432,993 + SO0416 0.81 +0.9 433,001 - SO0416 0.81 +0.8 433,080 + SO0416 0.86 +1.4 433,098 + SO0416 0.87 -1.1 433,112 + SO0416 0.88 +1.5 433,140 - SO0416 0.90 -2.5 433,152 + +1.6 433,183 - +2.1 433,185 + -1.2 433,193 - -3.9 433,193 - +0.6 433,291 + +0.3 433,291 + -0.9 433,296 + +0.3 433,305 - -0.5 433,343 + -1.9 433,361 - +3.0 433,361 - -0.3 433,437 - SO0417 0.11 +3.0 433,444 - SO0417 0.13 +2.7 433,450 + SO0417 0.14 +2.6 433,455 + SO0417 0.15 +0.9 433,525 - SO0417 0.32 -2.3 433,546 + SO0417 0.37 -1.8 433,546 + SO0417 0.37 +2.4 433,554 - SO0417 0.39 +5.0 433,604 + SO0417 0.51 +2.6 433,634 - +0.1 433,815 + +1.5 433,815 + +0.8 433,815 + +1.0 433,815 + +1.2 433,817 + +1.9 433,823 - -2.2 433,825 - +0.6 434,106 + +1.5 434,106 + -0.7 434,136 + -0.8 434,169 + +0.1 434,311 + -1.5
Or see this region's nucleotide sequence