Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0412 and SO0413 overlap by 8 nucleotides SO0413 and pilD are separated by 153 nucleotides pilD and SO0415 are separated by 165 nucleotides
SO0412: SO0412 - conserved hypothetical protein (NCBI ptt file), at 427,495 to 428,229
SO0412
SO0413: SO0413 - kinase, putative (NCBI ptt file), at 428,222 to 428,839
SO0413
SO0414: pilD - type 4 prepilin-like proteins leader peptide processing enzyme (NCBI ptt file), at 428,993 to 429,919
pilD
SO0415: SO0415 - type IV pilus biogenesis protein PilC (NCBI ptt file), at 430,085 to 431,356
SO0415
Position (kb) 428
429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 428.053 kb on - strand, within SO0412 at 428.067 kb on + strand, within SO0412 at 428.094 kb on - strand, within SO0412 at 428.144 kb on + strand, within SO0412 at 428.147 kb on - strand, within SO0412 at 428.152 kb on - strand, within SO0412 at 428.152 kb on - strand, within SO0412 at 428.198 kb on + strand at 428.198 kb on + strand at 428.225 kb on + strand at 428.898 kb on - strand at 428.987 kb on - strand at 428.988 kb on - strand at 429.070 kb on + strand at 429.105 kb on - strand, within pilD at 429.337 kb on + strand, within pilD at 429.355 kb on + strand, within pilD at 429.363 kb on - strand, within pilD at 429.511 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.598 kb on - strand, within pilD at 429.688 kb on - strand, within pilD at 429.993 kb on - strand at 430.106 kb on - strand at 430.126 kb on - strand at 430.143 kb on - strand at 430.217 kb on - strand, within SO0415 at 430.236 kb on + strand, within SO0415 at 430.236 kb on + strand, within SO0415 at 430.242 kb on + strand, within SO0415 at 430.268 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.318 kb on + strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.326 kb on - strand, within SO0415 at 430.541 kb on + strand, within SO0415 at 430.542 kb on + strand, within SO0415 at 430.542 kb on + strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.550 kb on - strand, within SO0415 at 430.552 kb on + strand, within SO0415 at 430.560 kb on - strand, within SO0415 at 430.562 kb on - strand, within SO0415 at 430.745 kb on + strand, within SO0415 at 430.745 kb on + strand, within SO0415 at 430.788 kb on + strand, within SO0415 at 430.841 kb on + strand, within SO0415 at 430.867 kb on + strand, within SO0415 at 430.896 kb on - strand, within SO0415
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 428,053 - SO0412 0.76 +1.2 428,067 + SO0412 0.78 +0.6 428,094 - SO0412 0.81 +0.2 428,144 + SO0412 0.88 -0.2 428,147 - SO0412 0.89 +0.8 428,152 - SO0412 0.89 -0.0 428,152 - SO0412 0.89 +0.6 428,198 + -2.1 428,198 + +0.5 428,225 + -1.3 428,898 - +3.0 428,987 - -0.6 428,988 - +2.1 429,070 + +2.4 429,105 - pilD SO0414 0.12 +1.8 429,337 + pilD SO0414 0.37 +4.0 429,355 + pilD SO0414 0.39 -1.2 429,363 - pilD SO0414 0.40 +0.1 429,511 - pilD SO0414 0.56 -1.6 429,588 - pilD SO0414 0.64 +0.3 429,588 - pilD SO0414 0.64 +3.7 429,598 - pilD SO0414 0.65 +2.0 429,688 - pilD SO0414 0.75 +2.4 429,993 - -1.9 430,106 - -0.6 430,126 - +1.2 430,143 - -2.5 430,217 - SO0415 0.10 +1.7 430,236 + SO0415 0.12 +0.1 430,236 + SO0415 0.12 +0.9 430,242 + SO0415 0.12 +2.8 430,268 + SO0415 0.14 -0.1 430,318 + SO0415 0.18 +1.9 430,318 + SO0415 0.18 +0.9 430,318 + SO0415 0.18 -1.9 430,326 - SO0415 0.19 -2.1 430,326 - SO0415 0.19 +0.9 430,326 - SO0415 0.19 -3.5 430,541 + SO0415 0.36 -1.3 430,542 + SO0415 0.36 +1.1 430,542 + SO0415 0.36 -1.3 430,550 - SO0415 0.37 +2.8 430,550 - SO0415 0.37 +0.9 430,550 - SO0415 0.37 -2.6 430,552 + SO0415 0.37 +1.4 430,560 - SO0415 0.37 -2.1 430,562 - SO0415 0.38 -0.2 430,745 + SO0415 0.52 -3.1 430,745 + SO0415 0.52 -0.3 430,788 + SO0415 0.55 +0.8 430,841 + SO0415 0.59 +1.5 430,867 + SO0415 0.61 -2.6 430,896 - SO0415 0.64 +0.8
Or see this region's nucleotide sequence