Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0411 and SO0412 are separated by 60 nucleotides SO0412 and SO0413 overlap by 8 nucleotides SO0413 and pilD are separated by 153 nucleotides
SO0411: SO0411 - conserved hypothetical protein (NCBI ptt file), at 427,225 to 427,434
SO0411
SO0412: SO0412 - conserved hypothetical protein (NCBI ptt file), at 427,495 to 428,229
SO0412
SO0413: SO0413 - kinase, putative (NCBI ptt file), at 428,222 to 428,839
SO0413
SO0414: pilD - type 4 prepilin-like proteins leader peptide processing enzyme (NCBI ptt file), at 428,993 to 429,919
pilD
Position (kb) 428
429 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 427.248 kb on - strand, within SO0411 at 427.263 kb on + strand, within SO0411 at 427.271 kb on - strand, within SO0411 at 427.278 kb on + strand, within SO0411 at 427.431 kb on + strand at 427.436 kb on + strand at 427.484 kb on + strand at 427.513 kb on + strand at 427.535 kb on + strand at 427.535 kb on + strand at 427.625 kb on + strand, within SO0412 at 427.625 kb on + strand, within SO0412 at 427.647 kb on + strand, within SO0412 at 427.647 kb on + strand, within SO0412 at 427.745 kb on - strand, within SO0412 at 427.747 kb on - strand, within SO0412 at 427.962 kb on - strand, within SO0412 at 427.963 kb on - strand, within SO0412 at 428.053 kb on - strand, within SO0412 at 428.067 kb on + strand, within SO0412 at 428.094 kb on - strand, within SO0412 at 428.144 kb on + strand, within SO0412 at 428.147 kb on - strand, within SO0412 at 428.152 kb on - strand, within SO0412 at 428.152 kb on - strand, within SO0412 at 428.198 kb on + strand at 428.198 kb on + strand at 428.225 kb on + strand at 428.898 kb on - strand at 428.987 kb on - strand at 428.988 kb on - strand at 429.070 kb on + strand at 429.105 kb on - strand, within pilD at 429.337 kb on + strand, within pilD at 429.355 kb on + strand, within pilD at 429.363 kb on - strand, within pilD at 429.511 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.588 kb on - strand, within pilD at 429.598 kb on - strand, within pilD at 429.688 kb on - strand, within pilD
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 427,248 - SO0411 0.11 +0.0 427,263 + SO0411 0.18 -0.0 427,271 - SO0411 0.22 -2.5 427,278 + SO0411 0.25 -0.4 427,431 + -1.6 427,436 + -0.9 427,484 + -2.6 427,513 + -0.7 427,535 + +0.1 427,535 + -1.5 427,625 + SO0412 0.18 +3.1 427,625 + SO0412 0.18 -0.1 427,647 + SO0412 0.21 +0.2 427,647 + SO0412 0.21 +2.7 427,745 - SO0412 0.34 +0.4 427,747 - SO0412 0.34 +0.2 427,962 - SO0412 0.64 +1.2 427,963 - SO0412 0.64 -2.5 428,053 - SO0412 0.76 +1.2 428,067 + SO0412 0.78 +0.6 428,094 - SO0412 0.81 +0.2 428,144 + SO0412 0.88 -0.2 428,147 - SO0412 0.89 +0.8 428,152 - SO0412 0.89 -0.0 428,152 - SO0412 0.89 +0.6 428,198 + -2.1 428,198 + +0.5 428,225 + -1.3 428,898 - +3.0 428,987 - -0.6 428,988 - +2.1 429,070 + +2.4 429,105 - pilD SO0414 0.12 +1.8 429,337 + pilD SO0414 0.37 +4.0 429,355 + pilD SO0414 0.39 -1.2 429,363 - pilD SO0414 0.40 +0.1 429,511 - pilD SO0414 0.56 -1.6 429,588 - pilD SO0414 0.64 +0.3 429,588 - pilD SO0414 0.64 +3.7 429,598 - pilD SO0414 0.65 +2.0 429,688 - pilD SO0414 0.75 +2.4
Or see this region's nucleotide sequence