Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0356 and SO0357 are separated by 106 nucleotides SO0357 and SO0358 are separated by 199 nucleotides SO0358 and spoT are separated by 24 nucleotides
SO0356: SO0356 - ISSod4, transposase (NCBI ptt file), at 364,485 to 365,687
SO0356
SO0357: SO0357 - metallo-beta-lactamase superfamily protein (NCBI ptt file), at 365,794 to 367,647
SO0357
SO0358: SO0358 - endoribonuclease L-PSP, putative (NCBI ptt file), at 367,847 to 368,230
SO0358
SO0359: spoT - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (NCBI ptt file), at 368,255 to 370,360
spoT
Position (kb) 365
366
367
368 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 365.728 kb on - strand at 365.728 kb on - strand at 365.872 kb on - strand at 365.876 kb on + strand at 365.884 kb on - strand at 365.919 kb on + strand at 365.919 kb on + strand at 365.919 kb on + strand at 365.919 kb on + strand at 365.919 kb on + strand at 365.927 kb on - strand at 365.927 kb on - strand at 365.927 kb on - strand at 365.927 kb on - strand at 365.935 kb on + strand at 365.935 kb on + strand at 365.960 kb on - strand at 365.966 kb on + strand at 365.966 kb on + strand at 365.966 kb on + strand at 365.974 kb on - strand at 365.974 kb on - strand at 365.974 kb on - strand at 365.974 kb on - strand at 365.974 kb on - strand at 365.991 kb on + strand, within SO0357 at 366.047 kb on + strand, within SO0357 at 366.090 kb on - strand, within SO0357 at 366.157 kb on + strand, within SO0357 at 366.159 kb on - strand, within SO0357 at 366.168 kb on - strand, within SO0357 at 366.175 kb on + strand, within SO0357 at 366.186 kb on + strand, within SO0357 at 366.186 kb on + strand, within SO0357 at 366.186 kb on + strand, within SO0357 at 366.186 kb on + strand, within SO0357 at 366.210 kb on + strand, within SO0357 at 366.218 kb on - strand, within SO0357 at 366.232 kb on - strand, within SO0357 at 366.283 kb on + strand, within SO0357 at 366.290 kb on + strand, within SO0357 at 366.352 kb on + strand, within SO0357 at 366.360 kb on - strand, within SO0357 at 366.368 kb on - strand, within SO0357 at 366.385 kb on + strand, within SO0357 at 366.385 kb on + strand, within SO0357 at 366.393 kb on - strand, within SO0357 at 366.412 kb on + strand, within SO0357 at 366.554 kb on - strand, within SO0357 at 366.569 kb on - strand, within SO0357 at 366.574 kb on + strand, within SO0357 at 366.642 kb on - strand, within SO0357 at 366.642 kb on - strand, within SO0357 at 366.657 kb on - strand, within SO0357 at 366.827 kb on - strand, within SO0357 at 366.906 kb on + strand, within SO0357 at 367.120 kb on - strand, within SO0357 at 367.177 kb on + strand, within SO0357 at 367.199 kb on - strand, within SO0357 at 367.237 kb on - strand, within SO0357 at 367.443 kb on - strand, within SO0357 at 367.465 kb on - strand at 367.640 kb on + strand at 367.765 kb on + strand at 367.783 kb on - strand at 367.815 kb on - strand at 367.845 kb on + strand at 367.845 kb on + strand at 367.897 kb on + strand, within SO0358 at 367.905 kb on - strand, within SO0358 at 367.923 kb on + strand, within SO0358 at 367.923 kb on + strand, within SO0358 at 367.931 kb on - strand, within SO0358 at 367.931 kb on - strand, within SO0358 at 367.931 kb on - strand, within SO0358 at 367.976 kb on + strand, within SO0358 at 367.983 kb on - strand, within SO0358 at 367.984 kb on - strand, within SO0358 at 367.984 kb on - strand, within SO0358 at 368.363 kb on + strand at 368.388 kb on - strand at 368.496 kb on - strand, within spoT at 368.581 kb on + strand, within spoT at 368.588 kb on + strand, within spoT
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 365,728 - -0.8 365,728 - +0.1 365,872 - -1.6 365,876 + -0.2 365,884 - +1.2 365,919 + +1.9 365,919 + -0.9 365,919 + +1.2 365,919 + +1.1 365,919 + -1.1 365,927 - -0.1 365,927 - +0.8 365,927 - +0.1 365,927 - -1.7 365,935 + -0.0 365,935 + +0.6 365,960 - +0.5 365,966 + -0.1 365,966 + +3.0 365,966 + +0.0 365,974 - -2.6 365,974 - -0.0 365,974 - -1.0 365,974 - -1.7 365,974 - -0.0 365,991 + SO0357 0.11 +1.1 366,047 + SO0357 0.14 -0.8 366,090 - SO0357 0.16 +0.2 366,157 + SO0357 0.20 +0.4 366,159 - SO0357 0.20 +0.0 366,168 - SO0357 0.20 +1.5 366,175 + SO0357 0.21 -1.8 366,186 + SO0357 0.21 +2.4 366,186 + SO0357 0.21 +0.6 366,186 + SO0357 0.21 -0.8 366,186 + SO0357 0.21 +0.1 366,210 + SO0357 0.22 +1.3 366,218 - SO0357 0.23 +0.1 366,232 - SO0357 0.24 -1.8 366,283 + SO0357 0.26 -2.0 366,290 + SO0357 0.27 -0.4 366,352 + SO0357 0.30 -0.9 366,360 - SO0357 0.31 +0.2 366,368 - SO0357 0.31 +1.0 366,385 + SO0357 0.32 +2.6 366,385 + SO0357 0.32 +0.4 366,393 - SO0357 0.32 +2.2 366,412 + SO0357 0.33 +1.3 366,554 - SO0357 0.41 -3.1 366,569 - SO0357 0.42 -0.1 366,574 + SO0357 0.42 +0.5 366,642 - SO0357 0.46 +0.2 366,642 - SO0357 0.46 +0.8 366,657 - SO0357 0.47 +0.6 366,827 - SO0357 0.56 +2.8 366,906 + SO0357 0.60 +0.3 367,120 - SO0357 0.72 +1.8 367,177 + SO0357 0.75 -0.3 367,199 - SO0357 0.76 -2.3 367,237 - SO0357 0.78 -1.7 367,443 - SO0357 0.89 +0.7 367,465 - +4.6 367,640 + +0.9 367,765 + -1.6 367,783 - -4.1 367,815 - +0.7 367,845 + +0.2 367,845 + -1.5 367,897 + SO0358 0.13 +1.2 367,905 - SO0358 0.15 +0.3 367,923 + SO0358 0.20 +2.2 367,923 + SO0358 0.20 +2.1 367,931 - SO0358 0.22 +0.8 367,931 - SO0358 0.22 +1.6 367,931 - SO0358 0.22 +0.7 367,976 + SO0358 0.34 +1.4 367,983 - SO0358 0.35 +2.2 367,984 - SO0358 0.36 +2.6 367,984 - SO0358 0.36 +3.6 368,363 + -1.3 368,388 - -3.2 368,496 - spoT SO0359 0.11 -0.2 368,581 + spoT SO0359 0.15 -0.8 368,588 + spoT SO0359 0.16 -1.9
Or see this region's nucleotide sequence