Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panK and SO0216 are separated by 112 nucleotides SO0216 and tRNA-Thr-2 overlap by 10 nucleotides tRNA-Thr-2 and tRNA-Tyr-1 are separated by 7 nucleotides tRNA-Tyr-1 and tRNA-Gly-6 are separated by 34 nucleotides tRNA-Gly-6 and tRNA-Thr-1 are separated by 9 nucleotides tRNA-Thr-1 and tufB are separated by 115 nucleotides tufB and secE are separated by 276 nucleotides secE and nusG are separated by 9 nucleotides
SO0215: panK - pantothenate kinase (NCBI ptt file), at 225,619 to 226,569
panK
SO0216: SO0216 - hypothetical protein (NCBI ptt file), at 226,682 to 226,813
SO0216
SO_t005: tRNA-Thr-2 - tRNA-Thr (RefSeq), at 226,804 to 226,879
tRNA-Thr-2
SO_t006: tRNA-Tyr-1 - tRNA-Tyr (RefSeq), at 226,887 to 226,971
tRNA-Tyr-1
SO_t007: tRNA-Gly-6 - tRNA-Gly (RefSeq), at 227,006 to 227,079
tRNA-Gly-6
SO_t008: tRNA-Thr-1 - tRNA-Thr (RefSeq), at 227,089 to 227,164
tRNA-Thr-1
SO0217: tufB - translation elongation factor Tu (NCBI ptt file), at 227,280 to 228,464
tufB
SO0218: secE - preprotein translocase, SecE subunit (NCBI ptt file), at 228,741 to 229,112
secE
SO0219: nusG - transcription antitermination protein NusG (NCBI ptt file), at 229,122 to 229,673
nusG
Position (kb) 227
228
229 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 226.604 kb on - strand at 226.676 kb on + strand at 226.704 kb on + strand, within SO0216 at 227.133 kb on + strand, within tRNA-Thr-1 at 228.121 kb on + strand, within tufB
Per-strain Table
Or see this region's nucleotide sequence