Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmA and murI are separated by 55 nucleotides murI and SO0208 are separated by 6 nucleotides SO0208 and Sp16SB are separated by 682 nucleotides
SO0206: trmA - tRNA (uracil-5-)-methyltransferase (NCBI ptt file), at 213,570 to 214,670
trmA
SO0207: murI - glutamate racemase (NCBI ptt file), at 214,726 to 215,634
murI
SO0208: SO0208 - RNA-binding protein (NCBI ptt file), at 215,641 to 216,105
SO0208
SO_r004: Sp16SB - 16S ribosomal RNA (RefSeq), at 216,788 to 218,316
Sp16SB
Position (kb) 215
216
217 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 214.687 kb on + strand at 214.695 kb on - strand at 214.721 kb on + strand at 214.733 kb on + strand at 215.700 kb on + strand, within SO0208 at 215.708 kb on - strand, within SO0208 at 215.762 kb on + strand, within SO0208 at 215.770 kb on - strand, within SO0208 at 215.770 kb on - strand, within SO0208 at 215.770 kb on - strand, within SO0208 at 215.782 kb on + strand, within SO0208 at 215.790 kb on - strand, within SO0208 at 215.839 kb on - strand, within SO0208 at 215.885 kb on - strand, within SO0208 at 215.893 kb on - strand, within SO0208 at 215.927 kb on + strand, within SO0208 at 215.950 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.041 kb on + strand, within SO0208 at 216.049 kb on - strand, within SO0208 at 216.243 kb on + strand at 216.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 214,687 + -0.8 214,695 - -0.9 214,721 + +0.4 214,733 + +1.3 215,700 + SO0208 0.13 -0.3 215,708 - SO0208 0.14 -3.4 215,762 + SO0208 0.26 -2.4 215,770 - SO0208 0.28 -1.8 215,770 - SO0208 0.28 +1.1 215,770 - SO0208 0.28 -0.4 215,782 + SO0208 0.30 -2.6 215,790 - SO0208 0.32 -1.9 215,839 - SO0208 0.43 -1.7 215,885 - SO0208 0.52 -1.4 215,893 - SO0208 0.54 -1.4 215,927 + SO0208 0.62 -1.5 215,950 + SO0208 0.66 -0.5 216,026 + SO0208 0.83 -3.0 216,026 + SO0208 0.83 -3.2 216,026 + SO0208 0.83 -1.6 216,026 + SO0208 0.83 -1.0 216,041 + SO0208 0.86 -0.6 216,049 - SO0208 0.88 -2.9 216,243 + -2.7 216,386 + -3.2
Or see this region's nucleotide sequence