Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0152 and SO0154 are separated by 241 nucleotides SO0154 and SO0157 are separated by 531 nucleotides
SO0152: SO0152 - hypothetical carboxypeptidase (NCBI ptt file), at 160,241 to 162,124
SO0152
SO0154: SO0154 - conserved hypothetical protein (NCBI ptt file), at 162,366 to 163,490
SO0154
SO0157: SO0157 - proton/glutamate symporter (NCBI ptt file), at 164,022 to 165,260
SO0157
Position (kb) 162
163
164 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 161.392 kb on + strand, within SO0152 at 161.419 kb on + strand, within SO0152 at 161.426 kb on + strand, within SO0152 at 161.427 kb on + strand, within SO0152 at 161.627 kb on + strand, within SO0152 at 161.666 kb on + strand, within SO0152 at 161.749 kb on - strand, within SO0152 at 161.765 kb on + strand, within SO0152 at 161.845 kb on + strand, within SO0152 at 161.846 kb on + strand, within SO0152 at 161.854 kb on - strand, within SO0152 at 161.881 kb on - strand, within SO0152 at 161.897 kb on + strand, within SO0152 at 161.897 kb on + strand, within SO0152 at 161.905 kb on - strand, within SO0152 at 161.912 kb on + strand, within SO0152 at 161.912 kb on + strand, within SO0152 at 161.926 kb on + strand, within SO0152 at 161.926 kb on + strand, within SO0152 at 162.005 kb on + strand at 162.053 kb on + strand at 162.061 kb on - strand at 162.061 kb on - strand at 162.061 kb on - strand at 162.122 kb on - strand at 162.171 kb on + strand at 162.343 kb on - strand at 162.452 kb on - strand at 162.488 kb on - strand, within SO0154 at 162.502 kb on - strand, within SO0154 at 162.535 kb on - strand, within SO0154 at 162.545 kb on + strand, within SO0154 at 162.566 kb on + strand, within SO0154 at 162.566 kb on + strand, within SO0154 at 162.566 kb on + strand, within SO0154 at 162.598 kb on + strand, within SO0154 at 162.659 kb on - strand, within SO0154 at 162.742 kb on + strand, within SO0154 at 162.754 kb on + strand, within SO0154 at 162.854 kb on + strand, within SO0154 at 162.945 kb on + strand, within SO0154 at 162.969 kb on - strand, within SO0154 at 162.987 kb on + strand, within SO0154 at 162.992 kb on - strand, within SO0154 at 163.089 kb on - strand, within SO0154 at 163.145 kb on - strand, within SO0154 at 163.287 kb on + strand, within SO0154 at 163.307 kb on + strand, within SO0154 at 163.403 kb on + strand at 163.411 kb on - strand at 163.415 kb on + strand at 163.423 kb on - strand at 163.833 kb on + strand at 163.873 kb on + strand at 163.881 kb on - strand at 163.887 kb on - strand at 163.902 kb on + strand at 163.902 kb on + strand at 163.906 kb on + strand at 163.915 kb on - strand at 164.001 kb on + strand at 164.001 kb on + strand at 164.003 kb on - strand at 164.003 kb on - strand at 164.178 kb on + strand, within SO0157 at 164.211 kb on + strand, within SO0157 at 164.272 kb on - strand, within SO0157 at 164.370 kb on - strand, within SO0157 at 164.407 kb on - strand, within SO0157 at 164.407 kb on - strand, within SO0157 at 164.407 kb on - strand, within SO0157
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 161,392 + SO0152 0.61 -2.3 161,419 + SO0152 0.63 +0.8 161,426 + SO0152 0.63 -0.0 161,427 + SO0152 0.63 +0.8 161,627 + SO0152 0.74 +1.7 161,666 + SO0152 0.76 -0.4 161,749 - SO0152 0.80 -0.6 161,765 + SO0152 0.81 -0.4 161,845 + SO0152 0.85 -1.2 161,846 + SO0152 0.85 -1.2 161,854 - SO0152 0.86 -1.0 161,881 - SO0152 0.87 +1.1 161,897 + SO0152 0.88 +0.8 161,897 + SO0152 0.88 -2.9 161,905 - SO0152 0.88 -1.4 161,912 + SO0152 0.89 +0.3 161,912 + SO0152 0.89 -0.0 161,926 + SO0152 0.89 +1.6 161,926 + SO0152 0.89 +1.8 162,005 + +0.5 162,053 + +0.0 162,061 - +0.9 162,061 - +0.7 162,061 - +0.5 162,122 - -1.2 162,171 + -1.3 162,343 - -1.9 162,452 - -2.3 162,488 - SO0154 0.11 +0.1 162,502 - SO0154 0.12 -0.4 162,535 - SO0154 0.15 -1.3 162,545 + SO0154 0.16 +0.3 162,566 + SO0154 0.18 +0.5 162,566 + SO0154 0.18 +0.3 162,566 + SO0154 0.18 -0.8 162,598 + SO0154 0.21 -2.1 162,659 - SO0154 0.26 +0.8 162,742 + SO0154 0.33 +2.4 162,754 + SO0154 0.34 +4.1 162,854 + SO0154 0.43 +1.3 162,945 + SO0154 0.51 +0.9 162,969 - SO0154 0.54 -0.9 162,987 + SO0154 0.55 +3.2 162,992 - SO0154 0.56 +2.1 163,089 - SO0154 0.64 -1.4 163,145 - SO0154 0.69 +0.3 163,287 + SO0154 0.82 -0.7 163,307 + SO0154 0.84 +1.5 163,403 + +2.4 163,411 - -1.0 163,415 + +0.6 163,423 - -0.2 163,833 + +1.3 163,873 + -1.5 163,881 - -2.3 163,887 - -2.2 163,902 + +0.5 163,902 + +1.0 163,906 + -0.3 163,915 - -1.2 164,001 + +0.2 164,001 + -2.4 164,003 - +0.8 164,003 - -0.2 164,178 + SO0157 0.13 +1.5 164,211 + SO0157 0.15 +0.7 164,272 - SO0157 0.20 +1.5 164,370 - SO0157 0.28 +1.6 164,407 - SO0157 0.31 -0.2 164,407 - SO0157 0.31 -0.5 164,407 - SO0157 0.31 -1.5
Or see this region's nucleotide sequence