Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0131 and SO0132 are separated by 101 nucleotides SO0132 and SO_0134 are separated by 230 nucleotides SO_0134 and SO0135 are separated by 42 nucleotides SO0135 and SO0136 are separated by 26 nucleotides
SO0131: SO0131 - hypothetical chorismate pyruvate lyase (NCBI ptt file), at 140,071 to 140,640
SO0131
SO0132: SO0132 - flagellar protein FliL, putative (NCBI ptt file), at 140,742 to 141,152
SO0132
SO_0134: SO_0134 - putative SAM-dependent methyltransferase (RefSeq), at 141,383 to 142,309
_0134
SO0135: SO0135 - hemolysin protein, putative (NCBI ptt file), at 142,352 to 142,642
SO0135
SO0136: SO0136 - hypothetical protein (NCBI ptt file), at 142,669 to 143,331
SO0136
Position (kb) 141
142
143 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.398 kb on + strand, within SO0131 at 140.406 kb on - strand, within SO0131 at 140.406 kb on - strand, within SO0131 at 140.501 kb on - strand, within SO0131 at 140.501 kb on - strand, within SO0131 at 140.628 kb on - strand at 140.729 kb on + strand at 140.729 kb on + strand at 140.779 kb on + strand at 140.781 kb on + strand at 140.781 kb on + strand at 140.786 kb on + strand, within SO0132 at 140.786 kb on + strand, within SO0132 at 140.789 kb on - strand, within SO0132 at 140.789 kb on - strand, within SO0132 at 140.794 kb on - strand, within SO0132 at 140.963 kb on + strand, within SO0132 at 140.971 kb on - strand, within SO0132 at 141.005 kb on - strand, within SO0132 at 141.045 kb on + strand, within SO0132 at 141.074 kb on + strand, within SO0132 at 141.101 kb on + strand, within SO0132 at 141.101 kb on + strand, within SO0132 at 141.101 kb on + strand, within SO0132 at 141.141 kb on - strand at 141.141 kb on - strand at 141.225 kb on + strand at 141.285 kb on - strand at 141.319 kb on + strand at 141.374 kb on - strand at 141.441 kb on - strand at 141.585 kb on + strand, within SO_0134 at 141.661 kb on + strand, within SO_0134 at 141.716 kb on - strand, within SO_0134 at 141.716 kb on - strand, within SO_0134 at 141.781 kb on + strand, within SO_0134 at 141.888 kb on + strand, within SO_0134 at 141.895 kb on - strand, within SO_0134 at 141.934 kb on + strand, within SO_0134 at 141.956 kb on - strand, within SO_0134 at 141.956 kb on - strand, within SO_0134 at 141.971 kb on - strand, within SO_0134 at 142.015 kb on - strand, within SO_0134 at 142.036 kb on + strand, within SO_0134 at 142.103 kb on + strand, within SO_0134 at 142.109 kb on + strand, within SO_0134 at 142.125 kb on + strand, within SO_0134 at 142.169 kb on - strand, within SO_0134 at 142.312 kb on - strand at 142.312 kb on - strand at 142.438 kb on - strand, within SO0135 at 142.453 kb on + strand, within SO0135 at 142.491 kb on + strand, within SO0135 at 142.550 kb on - strand, within SO0135 at 142.556 kb on - strand, within SO0135 at 142.701 kb on + strand at 142.906 kb on + strand, within SO0136 at 143.146 kb on - strand, within SO0136 at 143.177 kb on - strand, within SO0136
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 140,398 + SO0131 0.57 +2.6 140,398 + SO0131 0.57 +2.1 140,398 + SO0131 0.57 +1.1 140,398 + SO0131 0.57 -2.0 140,398 + SO0131 0.57 +0.9 140,398 + SO0131 0.57 -0.2 140,398 + SO0131 0.57 +0.7 140,398 + SO0131 0.57 -0.0 140,398 + SO0131 0.57 +0.7 140,406 - SO0131 0.59 -3.0 140,406 - SO0131 0.59 -1.3 140,501 - SO0131 0.75 -1.9 140,501 - SO0131 0.75 +0.6 140,628 - -0.9 140,729 + +2.2 140,729 + +0.9 140,779 + -0.7 140,781 + -2.5 140,781 + +0.2 140,786 + SO0132 0.11 +3.1 140,786 + SO0132 0.11 -3.5 140,789 - SO0132 0.11 +0.4 140,789 - SO0132 0.11 +1.2 140,794 - SO0132 0.13 +2.3 140,963 + SO0132 0.54 +0.8 140,971 - SO0132 0.56 +2.6 141,005 - SO0132 0.64 +1.2 141,045 + SO0132 0.74 -1.1 141,074 + SO0132 0.81 +3.1 141,101 + SO0132 0.87 +2.2 141,101 + SO0132 0.87 -0.2 141,101 + SO0132 0.87 +3.0 141,141 - -0.3 141,141 - +0.4 141,225 + +0.8 141,285 - -1.5 141,319 + -1.9 141,374 - +2.6 141,441 - +0.1 141,585 + SO_0134 0.22 +1.3 141,661 + SO_0134 0.30 -0.4 141,716 - SO_0134 0.36 -3.5 141,716 - SO_0134 0.36 -0.4 141,781 + SO_0134 0.43 +3.3 141,888 + SO_0134 0.54 +0.9 141,895 - SO_0134 0.55 -0.7 141,934 + SO_0134 0.59 -2.6 141,956 - SO_0134 0.62 -0.4 141,956 - SO_0134 0.62 -1.3 141,971 - SO_0134 0.63 -0.2 142,015 - SO_0134 0.68 +0.4 142,036 + SO_0134 0.70 +1.4 142,103 + SO_0134 0.78 -0.1 142,109 + SO_0134 0.78 +0.6 142,125 + SO_0134 0.80 +0.7 142,169 - SO_0134 0.85 +0.5 142,312 - +1.2 142,312 - +1.0 142,438 - SO0135 0.30 -3.3 142,453 + SO0135 0.35 -2.7 142,491 + SO0135 0.48 +1.5 142,550 - SO0135 0.68 -0.7 142,556 - SO0135 0.70 +1.2 142,701 + +1.4 142,906 + SO0136 0.36 -1.7 143,146 - SO0136 0.72 -0.8 143,177 - SO0136 0.77 +0.4
Or see this region's nucleotide sequence