Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0123 and SO0125 are separated by 211 nucleotides SO0125 and SO0126 are separated by 21 nucleotides SO0126 and SO0127 are separated by 62 nucleotides
SO0123: SO0123 - acyltransferase family protein (NCBI ptt file), at 132,352 to 132,912
SO0123
SO0125: SO0125 - conserved hypothetical protein (NCBI ptt file), at 133,124 to 133,489
SO0125
SO0126: SO0126 - conserved hypothetical protein (NCBI ptt file), at 133,511 to 134,311
SO0126
SO0127: SO0127 - hypothetical protein (NCBI ptt file), at 134,374 to 135,888
SO0127
Position (kb) 133
134
135 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 132.518 kb on + strand, within SO0123 at 132.526 kb on - strand, within SO0123 at 132.746 kb on - strand, within SO0123 at 132.805 kb on + strand, within SO0123 at 132.895 kb on + strand at 132.903 kb on - strand at 132.929 kb on - strand at 132.986 kb on + strand at 132.995 kb on - strand at 133.002 kb on + strand at 133.010 kb on - strand at 133.010 kb on - strand at 133.035 kb on + strand at 133.043 kb on - strand at 133.114 kb on + strand at 133.114 kb on + strand at 133.122 kb on - strand at 133.122 kb on - strand at 133.122 kb on - strand at 133.225 kb on + strand, within SO0125 at 133.233 kb on - strand, within SO0125 at 133.233 kb on - strand, within SO0125 at 133.242 kb on - strand, within SO0125 at 133.265 kb on + strand, within SO0125 at 133.265 kb on + strand, within SO0125 at 133.273 kb on - strand, within SO0125 at 133.317 kb on - strand, within SO0125 at 133.410 kb on + strand, within SO0125 at 133.410 kb on + strand, within SO0125 at 133.421 kb on + strand, within SO0125 at 133.467 kb on + strand at 133.487 kb on - strand at 133.587 kb on + strand at 133.592 kb on + strand, within SO0126 at 133.732 kb on - strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.797 kb on + strand, within SO0126 at 133.805 kb on - strand, within SO0126 at 133.805 kb on - strand, within SO0126 at 133.846 kb on - strand, within SO0126 at 133.937 kb on + strand, within SO0126 at 133.954 kb on - strand, within SO0126 at 133.984 kb on - strand, within SO0126 at 134.022 kb on - strand, within SO0126 at 134.101 kb on + strand, within SO0126 at 134.101 kb on + strand, within SO0126 at 134.118 kb on + strand, within SO0126 at 134.124 kb on + strand, within SO0126 at 134.128 kb on + strand, within SO0126 at 134.337 kb on + strand at 134.338 kb on + strand at 134.340 kb on - strand at 134.345 kb on - strand at 134.350 kb on - strand at 134.379 kb on - strand at 134.383 kb on + strand at 134.423 kb on - strand at 134.434 kb on + strand at 134.434 kb on + strand at 134.452 kb on - strand at 134.482 kb on + strand at 134.482 kb on + strand at 134.562 kb on + strand, within SO0127 at 134.631 kb on + strand, within SO0127 at 134.631 kb on + strand, within SO0127 at 134.639 kb on - strand, within SO0127 at 134.740 kb on - strand, within SO0127 at 134.741 kb on - strand, within SO0127 at 134.788 kb on - strand, within SO0127 at 134.858 kb on + strand, within SO0127 at 134.910 kb on + strand, within SO0127 at 134.940 kb on + strand, within SO0127 at 134.940 kb on + strand, within SO0127 at 134.940 kb on + strand, within SO0127 at 134.940 kb on + strand, within SO0127 at 134.948 kb on - strand, within SO0127 at 134.955 kb on - strand, within SO0127 at 135.015 kb on + strand, within SO0127 at 135.090 kb on - strand, within SO0127 at 135.090 kb on - strand, within SO0127 at 135.097 kb on - strand, within SO0127 at 135.099 kb on + strand, within SO0127 at 135.105 kb on + strand, within SO0127 at 135.105 kb on + strand, within SO0127 at 135.107 kb on - strand, within SO0127 at 135.149 kb on - strand, within SO0127 at 135.182 kb on + strand, within SO0127 at 135.190 kb on - strand, within SO0127 at 135.267 kb on + strand, within SO0127 at 135.268 kb on - strand, within SO0127 at 135.268 kb on - strand, within SO0127 at 135.280 kb on - strand, within SO0127 at 135.280 kb on - strand, within SO0127
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 132,518 + SO0123 0.30 -0.0 132,526 - SO0123 0.31 +0.8 132,746 - SO0123 0.70 +0.1 132,805 + SO0123 0.81 -0.6 132,895 + -3.2 132,903 - -1.0 132,929 - +1.1 132,986 + +0.8 132,995 - -1.6 133,002 + +0.3 133,010 - +0.7 133,010 - -0.4 133,035 + +1.9 133,043 - -1.5 133,114 + -3.0 133,114 + +1.1 133,122 - +0.6 133,122 - +0.1 133,122 - -1.5 133,225 + SO0125 0.28 -0.3 133,233 - SO0125 0.30 +1.8 133,233 - SO0125 0.30 -0.5 133,242 - SO0125 0.32 +0.4 133,265 + SO0125 0.39 +0.4 133,265 + SO0125 0.39 -1.5 133,273 - SO0125 0.41 -2.4 133,317 - SO0125 0.53 -0.6 133,410 + SO0125 0.78 +0.1 133,410 + SO0125 0.78 -3.2 133,421 + SO0125 0.81 +0.4 133,467 + -0.9 133,487 - -0.2 133,587 + +1.2 133,592 + SO0126 0.10 +0.0 133,732 - SO0126 0.28 -2.6 133,797 + SO0126 0.36 -0.0 133,797 + SO0126 0.36 -0.1 133,797 + SO0126 0.36 -0.1 133,797 + SO0126 0.36 +0.9 133,797 + SO0126 0.36 +0.5 133,805 - SO0126 0.37 +0.6 133,805 - SO0126 0.37 +0.9 133,846 - SO0126 0.42 +1.1 133,937 + SO0126 0.53 +2.4 133,954 - SO0126 0.55 +1.0 133,984 - SO0126 0.59 +0.2 134,022 - SO0126 0.64 -2.0 134,101 + SO0126 0.74 -1.6 134,101 + SO0126 0.74 -0.4 134,118 + SO0126 0.76 -2.4 134,124 + SO0126 0.77 +1.9 134,128 + SO0126 0.77 -0.5 134,337 + -1.9 134,338 + -2.5 134,340 - -0.8 134,345 - +1.5 134,350 - -1.3 134,379 - -2.8 134,383 + -1.7 134,423 - -1.4 134,434 + -5.0 134,434 + -3.3 134,452 - -1.0 134,482 + -1.7 134,482 + -3.5 134,562 + SO0127 0.12 -1.9 134,631 + SO0127 0.17 -2.2 134,631 + SO0127 0.17 -5.4 134,639 - SO0127 0.17 -3.5 134,740 - SO0127 0.24 -4.3 134,741 - SO0127 0.24 -4.2 134,788 - SO0127 0.27 -1.5 134,858 + SO0127 0.32 -4.0 134,910 + SO0127 0.35 -2.5 134,940 + SO0127 0.37 -4.8 134,940 + SO0127 0.37 -3.9 134,940 + SO0127 0.37 -1.3 134,940 + SO0127 0.37 -1.9 134,948 - SO0127 0.38 -4.3 134,955 - SO0127 0.38 -2.2 135,015 + SO0127 0.42 -2.4 135,090 - SO0127 0.47 -1.7 135,090 - SO0127 0.47 -1.0 135,097 - SO0127 0.48 -2.7 135,099 + SO0127 0.48 -2.3 135,105 + SO0127 0.48 -2.2 135,105 + SO0127 0.48 -4.3 135,107 - SO0127 0.48 -1.9 135,149 - SO0127 0.51 -2.8 135,182 + SO0127 0.53 -2.5 135,190 - SO0127 0.54 -1.9 135,267 + SO0127 0.59 -3.7 135,268 - SO0127 0.59 -2.2 135,268 - SO0127 0.59 -3.5 135,280 - SO0127 0.60 -3.0 135,280 - SO0127 0.60 -1.0
Or see this region's nucleotide sequence