Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0110 and SO0111 are separated by 115 nucleotides SO0111 and SO0112 are separated by 281 nucleotides SO0112 and SO0113 are separated by 0 nucleotides
SO0110: SO0110 - hypothetical protease (NCBI ptt file), at 120,287 to 121,630
SO0110
SO0111: SO0111 - hypothetical protein (NCBI ptt file), at 121,746 to 122,360
SO0111
SO0112: SO0112 - conserved hypothetical protein (NCBI ptt file), at 122,642 to 123,265
SO0112
SO0113: SO0113 - conserved hypothetical protein (NCBI ptt file), at 123,266 to 124,222
SO0113
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 120.768 kb on - strand, within SO0110 at 120.790 kb on - strand, within SO0110 at 120.833 kb on - strand, within SO0110 at 120.848 kb on - strand, within SO0110 at 120.851 kb on + strand, within SO0110 at 120.864 kb on - strand, within SO0110 at 120.881 kb on - strand, within SO0110 at 120.897 kb on + strand, within SO0110 at 120.900 kb on - strand, within SO0110 at 120.900 kb on - strand, within SO0110 at 120.972 kb on + strand, within SO0110 at 120.991 kb on + strand, within SO0110 at 120.999 kb on - strand, within SO0110 at 121.000 kb on - strand, within SO0110 at 121.059 kb on - strand, within SO0110 at 121.081 kb on - strand, within SO0110 at 121.081 kb on - strand, within SO0110 at 121.091 kb on + strand, within SO0110 at 121.091 kb on + strand, within SO0110 at 121.091 kb on + strand, within SO0110 at 121.099 kb on - strand, within SO0110 at 121.099 kb on - strand, within SO0110 at 121.099 kb on - strand, within SO0110 at 121.099 kb on - strand, within SO0110 at 121.099 kb on - strand, within SO0110 at 121.177 kb on + strand, within SO0110 at 121.177 kb on + strand, within SO0110 at 121.188 kb on + strand, within SO0110 at 121.246 kb on + strand, within SO0110 at 121.249 kb on - strand, within SO0110 at 121.254 kb on - strand, within SO0110 at 121.312 kb on + strand, within SO0110 at 121.385 kb on + strand, within SO0110 at 121.387 kb on + strand, within SO0110 at 121.395 kb on - strand, within SO0110 at 121.412 kb on + strand, within SO0110 at 121.414 kb on + strand, within SO0110 at 121.421 kb on - strand, within SO0110 at 121.427 kb on + strand, within SO0110 at 121.427 kb on + strand, within SO0110 at 121.435 kb on - strand, within SO0110 at 121.449 kb on - strand, within SO0110 at 121.481 kb on - strand, within SO0110 at 121.505 kb on + strand at 121.505 kb on + strand at 121.513 kb on - strand at 121.515 kb on + strand at 121.515 kb on + strand at 121.515 kb on + strand at 121.515 kb on + strand at 121.515 kb on + strand at 121.515 kb on + strand at 121.523 kb on - strand at 121.523 kb on - strand at 121.523 kb on - strand at 121.523 kb on - strand at 121.523 kb on - strand at 121.523 kb on - strand at 121.528 kb on - strand at 121.578 kb on + strand at 121.586 kb on - strand at 121.596 kb on + strand at 121.604 kb on - strand at 121.611 kb on + strand at 121.700 kb on + strand at 121.865 kb on - strand, within SO0111 at 121.917 kb on - strand, within SO0111 at 122.007 kb on - strand, within SO0111 at 122.020 kb on + strand, within SO0111 at 122.028 kb on - strand, within SO0111 at 122.071 kb on + strand, within SO0111 at 122.154 kb on + strand, within SO0111 at 122.171 kb on + strand, within SO0111 at 122.171 kb on + strand, within SO0111 at 122.171 kb on + strand, within SO0111 at 122.171 kb on + strand, within SO0111 at 122.179 kb on - strand, within SO0111 at 122.179 kb on - strand, within SO0111 at 122.232 kb on + strand, within SO0111 at 122.419 kb on - strand at 122.468 kb on + strand at 122.468 kb on + strand at 122.476 kb on - strand at 122.476 kb on - strand at 122.491 kb on + strand at 122.533 kb on - strand at 122.535 kb on + strand at 122.535 kb on + strand at 122.535 kb on + strand at 122.543 kb on - strand at 122.543 kb on - strand at 122.543 kb on - strand at 122.543 kb on - strand at 122.543 kb on - strand at 122.543 kb on - strand at 122.582 kb on + strand at 122.615 kb on - strand at 122.621 kb on - strand at 122.621 kb on - strand at 122.702 kb on + strand at 122.738 kb on + strand, within SO0112 at 122.756 kb on - strand, within SO0112 at 122.777 kb on - strand, within SO0112 at 122.878 kb on + strand, within SO0112 at 122.878 kb on + strand, within SO0112 at 122.886 kb on - strand, within SO0112 at 122.901 kb on + strand, within SO0112 at 122.909 kb on - strand, within SO0112 at 123.033 kb on - strand, within SO0112 at 123.137 kb on - strand, within SO0112 at 123.323 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 120,768 - SO0110 0.36 -1.3 120,790 - SO0110 0.37 +0.5 120,833 - SO0110 0.41 +0.7 120,848 - SO0110 0.42 -2.0 120,851 + SO0110 0.42 +1.6 120,864 - SO0110 0.43 -0.0 120,881 - SO0110 0.44 +0.4 120,897 + SO0110 0.45 -2.0 120,900 - SO0110 0.46 +0.1 120,900 - SO0110 0.46 -0.4 120,972 + SO0110 0.51 +0.4 120,991 + SO0110 0.52 -1.5 120,999 - SO0110 0.53 +1.6 121,000 - SO0110 0.53 -3.6 121,059 - SO0110 0.57 -1.2 121,081 - SO0110 0.59 -2.6 121,081 - SO0110 0.59 +0.9 121,091 + SO0110 0.60 +0.3 121,091 + SO0110 0.60 +2.0 121,091 + SO0110 0.60 +0.3 121,099 - SO0110 0.60 +1.4 121,099 - SO0110 0.60 -0.3 121,099 - SO0110 0.60 -0.3 121,099 - SO0110 0.60 +0.8 121,099 - SO0110 0.60 -0.1 121,177 + SO0110 0.66 -0.5 121,177 + SO0110 0.66 +0.7 121,188 + SO0110 0.67 -0.1 121,246 + SO0110 0.71 -1.1 121,249 - SO0110 0.72 +0.0 121,254 - SO0110 0.72 +2.1 121,312 + SO0110 0.76 -1.8 121,385 + SO0110 0.82 -0.0 121,387 + SO0110 0.82 -1.1 121,395 - SO0110 0.82 -0.2 121,412 + SO0110 0.84 +1.1 121,414 + SO0110 0.84 -0.2 121,421 - SO0110 0.84 -0.3 121,427 + SO0110 0.85 +1.5 121,427 + SO0110 0.85 +1.5 121,435 - SO0110 0.85 +1.8 121,449 - SO0110 0.86 -2.0 121,481 - SO0110 0.89 -0.4 121,505 + +0.4 121,505 + -1.1 121,513 - -1.5 121,515 + -0.1 121,515 + -1.7 121,515 + -1.1 121,515 + +0.3 121,515 + +0.2 121,515 + +0.9 121,523 - -0.0 121,523 - -2.6 121,523 - -0.2 121,523 - +1.5 121,523 - -1.8 121,523 - -3.5 121,528 - -0.2 121,578 + +1.0 121,586 - -0.1 121,596 + +1.7 121,604 - -2.8 121,611 + -0.7 121,700 + +1.6 121,865 - SO0111 0.19 +0.0 121,917 - SO0111 0.28 -1.2 122,007 - SO0111 0.42 -0.8 122,020 + SO0111 0.45 -0.1 122,028 - SO0111 0.46 +0.3 122,071 + SO0111 0.53 -0.7 122,154 + SO0111 0.66 +1.1 122,171 + SO0111 0.69 +0.1 122,171 + SO0111 0.69 -1.5 122,171 + SO0111 0.69 -0.0 122,171 + SO0111 0.69 +0.4 122,179 - SO0111 0.70 +0.9 122,179 - SO0111 0.70 -0.6 122,232 + SO0111 0.79 +0.1 122,419 - +2.1 122,468 + -3.0 122,468 + +1.0 122,476 - -0.2 122,476 - -1.1 122,491 + +0.1 122,533 - -0.3 122,535 + -0.7 122,535 + -0.7 122,535 + -0.4 122,543 - -0.7 122,543 - -3.0 122,543 - +2.1 122,543 - -2.4 122,543 - -1.5 122,543 - +0.8 122,582 + -1.2 122,615 - -2.4 122,621 - -1.1 122,621 - -0.1 122,702 + +1.8 122,738 + SO0112 0.15 +1.3 122,756 - SO0112 0.18 +0.8 122,777 - SO0112 0.22 +1.0 122,878 + SO0112 0.38 -2.9 122,878 + SO0112 0.38 +0.5 122,886 - SO0112 0.39 -0.7 122,901 + SO0112 0.42 -1.0 122,909 - SO0112 0.43 +1.3 123,033 - SO0112 0.63 +0.9 123,137 - SO0112 0.79 +1.9 123,323 + -0.8
Or see this region's nucleotide sequence