Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0061 and SO0062 are separated by 333 nucleotides SO0062 and SO0063 overlap by 4 nucleotides
SO0061: SO0061 - lipoprotein, NLP/P60 family (NCBI ptt file), at 68,407 to 68,871
SO0061
SO0062: SO0062 - hypothetical protein (NCBI ptt file), at 69,205 to 69,591
SO0062
SO0063: SO0063 - conserved hypothetical protein (NCBI ptt file), at 69,588 to 70,895
SO0063
Position (kb)
69
70 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 68.297 kb on + strand at 68.310 kb on - strand at 68.328 kb on - strand at 68.368 kb on + strand at 68.373 kb on + strand at 68.387 kb on - strand at 68.389 kb on + strand at 68.442 kb on - strand at 68.442 kb on - strand at 68.469 kb on + strand, within SO0061 at 68.469 kb on + strand, within SO0061 at 68.469 kb on + strand, within SO0061 at 68.469 kb on + strand, within SO0061 at 68.469 kb on + strand, within SO0061 at 68.469 kb on + strand, within SO0061 at 68.476 kb on - strand, within SO0061 at 68.477 kb on - strand, within SO0061 at 68.477 kb on - strand, within SO0061 at 68.477 kb on - strand, within SO0061 at 68.477 kb on - strand, within SO0061 at 68.517 kb on + strand, within SO0061 at 68.530 kb on + strand, within SO0061 at 68.565 kb on - strand, within SO0061 at 68.610 kb on - strand, within SO0061 at 68.634 kb on + strand, within SO0061 at 68.644 kb on - strand, within SO0061 at 68.654 kb on + strand, within SO0061 at 68.676 kb on + strand, within SO0061 at 68.741 kb on - strand, within SO0061 at 68.744 kb on + strand, within SO0061 at 68.823 kb on + strand, within SO0061 at 68.823 kb on + strand, within SO0061 at 68.823 kb on + strand, within SO0061 at 68.831 kb on + strand at 68.832 kb on + strand at 68.872 kb on + strand at 68.882 kb on + strand at 68.902 kb on + strand at 68.902 kb on + strand at 68.902 kb on + strand at 68.910 kb on - strand at 68.910 kb on - strand at 68.939 kb on + strand at 68.956 kb on + strand at 69.170 kb on - strand at 69.207 kb on - strand at 69.370 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.372 kb on - strand, within SO0062 at 69.497 kb on - strand, within SO0062 at 69.501 kb on + strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.509 kb on - strand, within SO0062 at 69.564 kb on - strand at 69.587 kb on + strand at 69.606 kb on + strand at 69.611 kb on + strand at 69.612 kb on + strand at 69.658 kb on + strand at 69.673 kb on - strand at 69.675 kb on - strand at 69.695 kb on + strand at 69.727 kb on + strand, within SO0063 at 69.727 kb on + strand, within SO0063 at 69.745 kb on + strand, within SO0063 at 69.745 kb on + strand, within SO0063 at 69.753 kb on - strand, within SO0063 at 69.797 kb on - strand, within SO0063 at 69.797 kb on - strand, within SO0063 at 69.799 kb on - strand, within SO0063 at 69.917 kb on + strand, within SO0063 at 69.917 kb on + strand, within SO0063 at 69.943 kb on + strand, within SO0063 at 69.973 kb on + strand, within SO0063 at 69.973 kb on + strand, within SO0063 at 69.974 kb on + strand, within SO0063 at 69.981 kb on - strand, within SO0063 at 69.981 kb on - strand, within SO0063 at 69.982 kb on - strand, within SO0063 at 70.097 kb on + strand, within SO0063 at 70.117 kb on - strand, within SO0063 at 70.140 kb on + strand, within SO0063 at 70.199 kb on + strand, within SO0063 at 70.202 kb on + strand, within SO0063 at 70.202 kb on + strand, within SO0063 at 70.210 kb on - strand, within SO0063 at 70.210 kb on - strand, within SO0063 at 70.210 kb on - strand, within SO0063 at 70.210 kb on - strand, within SO0063 at 70.224 kb on - strand, within SO0063 at 70.245 kb on + strand, within SO0063 at 70.245 kb on - strand, within SO0063 at 70.253 kb on - strand, within SO0063 at 70.260 kb on + strand, within SO0063 at 70.292 kb on + strand, within SO0063 at 70.349 kb on - strand, within SO0063 at 70.356 kb on - strand, within SO0063 at 70.479 kb on - strand at 70.532 kb on - strand, within SO0063 at 70.571 kb on + strand, within SO0063
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 68,297 + -2.0 68,310 - +1.3 68,328 - -0.1 68,368 + -1.7 68,373 + -1.6 68,387 - -1.2 68,389 + -1.1 68,442 - +0.6 68,442 - +0.8 68,469 + SO0061 0.13 -2.8 68,469 + SO0061 0.13 -1.0 68,469 + SO0061 0.13 +0.4 68,469 + SO0061 0.13 -0.1 68,469 + SO0061 0.13 +2.5 68,469 + SO0061 0.13 -1.0 68,476 - SO0061 0.15 +2.7 68,477 - SO0061 0.15 +1.7 68,477 - SO0061 0.15 +1.4 68,477 - SO0061 0.15 -1.1 68,477 - SO0061 0.15 -0.8 68,517 + SO0061 0.24 -1.1 68,530 + SO0061 0.26 +0.8 68,565 - SO0061 0.34 -2.4 68,610 - SO0061 0.44 -1.1 68,634 + SO0061 0.49 +1.5 68,644 - SO0061 0.51 -0.7 68,654 + SO0061 0.53 -0.8 68,676 + SO0061 0.58 +0.5 68,741 - SO0061 0.72 +1.8 68,744 + SO0061 0.72 -2.6 68,823 + SO0061 0.89 +0.9 68,823 + SO0061 0.89 -0.6 68,823 + SO0061 0.89 -0.3 68,831 + -0.1 68,832 + -0.9 68,872 + -1.4 68,882 + -0.3 68,902 + +1.7 68,902 + -0.5 68,902 + -2.0 68,910 - -0.4 68,910 - +0.6 68,939 + -1.5 68,956 + -1.5 69,170 - -4.0 69,207 - -2.5 69,370 - SO0062 0.43 -1.2 69,372 - SO0062 0.43 -4.2 69,372 - SO0062 0.43 -2.7 69,372 - SO0062 0.43 -3.4 69,372 - SO0062 0.43 -3.8 69,372 - SO0062 0.43 -4.2 69,372 - SO0062 0.43 -3.0 69,372 - SO0062 0.43 -4.4 69,372 - SO0062 0.43 -4.3 69,372 - SO0062 0.43 -3.8 69,497 - SO0062 0.75 -1.2 69,501 + SO0062 0.76 -1.0 69,509 - SO0062 0.79 -0.0 69,509 - SO0062 0.79 -3.9 69,509 - SO0062 0.79 -0.4 69,509 - SO0062 0.79 -0.0 69,509 - SO0062 0.79 -1.6 69,509 - SO0062 0.79 -2.3 69,509 - SO0062 0.79 -2.4 69,509 - SO0062 0.79 -3.1 69,564 - +1.9 69,587 + -0.3 69,606 + -0.9 69,611 + +2.9 69,612 + +1.0 69,658 + +0.5 69,673 - +0.5 69,675 - -3.5 69,695 + +1.1 69,727 + SO0063 0.11 -1.0 69,727 + SO0063 0.11 -2.3 69,745 + SO0063 0.12 -0.4 69,745 + SO0063 0.12 -1.1 69,753 - SO0063 0.13 +0.7 69,797 - SO0063 0.16 +0.2 69,797 - SO0063 0.16 +0.1 69,799 - SO0063 0.16 +1.5 69,917 + SO0063 0.25 -1.3 69,917 + SO0063 0.25 +1.6 69,943 + SO0063 0.27 +3.8 69,973 + SO0063 0.29 +2.2 69,973 + SO0063 0.29 +1.6 69,974 + SO0063 0.30 -0.3 69,981 - SO0063 0.30 +2.0 69,981 - SO0063 0.30 -0.6 69,982 - SO0063 0.30 -2.3 70,097 + SO0063 0.39 -2.1 70,117 - SO0063 0.40 +2.3 70,140 + SO0063 0.42 -2.2 70,199 + SO0063 0.47 +1.3 70,202 + SO0063 0.47 +0.2 70,202 + SO0063 0.47 +0.2 70,210 - SO0063 0.48 +1.9 70,210 - SO0063 0.48 +0.4 70,210 - SO0063 0.48 -1.7 70,210 - SO0063 0.48 -3.5 70,224 - SO0063 0.49 -1.5 70,245 + SO0063 0.50 -0.2 70,245 - SO0063 0.50 +3.2 70,253 - SO0063 0.51 +0.1 70,260 + SO0063 0.51 +2.1 70,292 + SO0063 0.54 -0.4 70,349 - SO0063 0.58 +0.1 70,356 - SO0063 0.59 +0.4 70,479 - +1.3 70,532 - SO0063 0.72 +0.1 70,571 + SO0063 0.75 -1.0
Or see this region's nucleotide sequence