Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fmt and def-1 are separated by 15 nucleotides def-1 and SO0033 are separated by 125 nucleotides SO0033 and SO0034 are separated by 83 nucleotides
SO0031: fmt - methionyl-tRNA formyltransferase (NCBI ptt file), at 36,420 to 37,376
fmt
SO0032: def-1 - polypeptide deformylase (NCBI ptt file), at 37,392 to 37,898
def-1
SO0033: SO0033 - LysM domain protein (NCBI ptt file), at 38,024 to 39,160
SO0033
SO0034: SO0034 - DNA processing protein DprA, putative (NCBI ptt file), at 39,244 to 40,260
SO0034
Position (kb) 38
39
40 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 37.454 kb on - strand, within def-1 at 37.488 kb on - strand, within def-1 at 37.488 kb on - strand, within def-1 at 37.563 kb on - strand, within def-1 at 37.576 kb on - strand, within def-1 at 37.601 kb on - strand, within def-1 at 37.637 kb on - strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.822 kb on + strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.830 kb on - strand, within def-1 at 37.834 kb on + strand, within def-1 at 37.875 kb on + strand at 37.883 kb on - strand at 37.947 kb on + strand at 37.955 kb on - strand at 37.955 kb on - strand at 37.955 kb on - strand at 38.001 kb on - strand at 38.068 kb on - strand at 38.068 kb on - strand at 38.260 kb on + strand, within SO0033 at 38.265 kb on + strand, within SO0033 at 38.273 kb on - strand, within SO0033 at 38.273 kb on - strand, within SO0033 at 38.273 kb on - strand, within SO0033 at 38.287 kb on + strand, within SO0033 at 38.287 kb on + strand, within SO0033 at 38.287 kb on + strand, within SO0033 at 38.295 kb on - strand, within SO0033 at 38.295 kb on - strand, within SO0033 at 38.295 kb on - strand, within SO0033 at 38.330 kb on + strand, within SO0033 at 38.434 kb on - strand, within SO0033 at 38.454 kb on + strand, within SO0033 at 38.454 kb on + strand, within SO0033 at 38.476 kb on - strand, within SO0033 at 38.476 kb on - strand, within SO0033 at 38.483 kb on + strand, within SO0033 at 38.485 kb on + strand, within SO0033 at 38.543 kb on + strand, within SO0033 at 38.577 kb on + strand, within SO0033 at 38.585 kb on - strand, within SO0033 at 38.603 kb on + strand, within SO0033 at 38.712 kb on + strand, within SO0033 at 38.779 kb on + strand, within SO0033 at 38.779 kb on + strand, within SO0033 at 38.779 kb on + strand, within SO0033 at 38.787 kb on - strand, within SO0033 at 38.787 kb on - strand, within SO0033 at 38.787 kb on - strand, within SO0033 at 38.787 kb on - strand, within SO0033 at 38.831 kb on + strand, within SO0033 at 38.836 kb on + strand, within SO0033 at 38.859 kb on + strand, within SO0033 at 38.862 kb on - strand, within SO0033 at 38.877 kb on - strand, within SO0033 at 38.882 kb on - strand, within SO0033 at 38.977 kb on - strand, within SO0033 at 38.992 kb on + strand, within SO0033 at 39.153 kb on - strand at 39.207 kb on + strand at 39.257 kb on + strand at 39.265 kb on - strand at 39.362 kb on + strand, within SO0034 at 39.408 kb on + strand, within SO0034 at 39.408 kb on + strand, within SO0034 at 39.416 kb on - strand, within SO0034 at 39.416 kb on - strand, within SO0034 at 39.416 kb on - strand, within SO0034 at 39.421 kb on - strand, within SO0034 at 39.470 kb on + strand, within SO0034 at 39.615 kb on - strand, within SO0034 at 39.616 kb on - strand, within SO0034 at 39.625 kb on + strand, within SO0034 at 39.625 kb on - strand, within SO0034 at 39.625 kb on - strand, within SO0034 at 39.633 kb on - strand, within SO0034 at 39.633 kb on - strand, within SO0034 at 39.633 kb on - strand, within SO0034 at 39.649 kb on + strand, within SO0034 at 39.661 kb on + strand, within SO0034 at 39.737 kb on + strand, within SO0034 at 39.763 kb on + strand, within SO0034 at 39.880 kb on - strand, within SO0034 at 40.061 kb on + strand, within SO0034
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 37,454 - def-1 SO0032 0.12 -1.2 37,488 - def-1 SO0032 0.19 -1.0 37,488 - def-1 SO0032 0.19 -1.1 37,563 - def-1 SO0032 0.34 -3.9 37,576 - def-1 SO0032 0.36 -1.1 37,601 - def-1 SO0032 0.41 -0.1 37,637 - def-1 SO0032 0.48 +0.1 37,822 + def-1 SO0032 0.85 -0.1 37,822 + def-1 SO0032 0.85 -2.0 37,822 + def-1 SO0032 0.85 -1.8 37,822 + def-1 SO0032 0.85 -0.2 37,822 + def-1 SO0032 0.85 -0.7 37,822 + def-1 SO0032 0.85 +0.3 37,822 + def-1 SO0032 0.85 -0.7 37,822 + def-1 SO0032 0.85 -1.0 37,822 + def-1 SO0032 0.85 -1.7 37,830 - def-1 SO0032 0.86 -0.5 37,830 - def-1 SO0032 0.86 -2.2 37,830 - def-1 SO0032 0.86 +0.6 37,830 - def-1 SO0032 0.86 -0.9 37,830 - def-1 SO0032 0.86 -1.2 37,830 - def-1 SO0032 0.86 -2.2 37,830 - def-1 SO0032 0.86 -2.6 37,830 - def-1 SO0032 0.86 -0.9 37,830 - def-1 SO0032 0.86 -1.2 37,830 - def-1 SO0032 0.86 +1.5 37,830 - def-1 SO0032 0.86 -1.4 37,834 + def-1 SO0032 0.87 +0.2 37,875 + -1.0 37,883 - -1.8 37,947 + -1.7 37,955 - +0.8 37,955 - +1.0 37,955 - +0.9 38,001 - +0.4 38,068 - +1.0 38,068 - +0.8 38,260 + SO0033 0.21 +1.0 38,265 + SO0033 0.21 +0.6 38,273 - SO0033 0.22 +0.0 38,273 - SO0033 0.22 +1.9 38,273 - SO0033 0.22 -2.3 38,287 + SO0033 0.23 +1.4 38,287 + SO0033 0.23 +0.8 38,287 + SO0033 0.23 +0.4 38,295 - SO0033 0.24 +1.1 38,295 - SO0033 0.24 -0.4 38,295 - SO0033 0.24 +0.8 38,330 + SO0033 0.27 +0.4 38,434 - SO0033 0.36 -2.0 38,454 + SO0033 0.38 -0.0 38,454 + SO0033 0.38 +0.5 38,476 - SO0033 0.40 +0.3 38,476 - SO0033 0.40 +1.0 38,483 + SO0033 0.40 -3.8 38,485 + SO0033 0.41 +2.0 38,543 + SO0033 0.46 +2.3 38,577 + SO0033 0.49 -1.8 38,585 - SO0033 0.49 +0.5 38,603 + SO0033 0.51 +0.7 38,712 + SO0033 0.61 +0.7 38,779 + SO0033 0.66 +1.2 38,779 + SO0033 0.66 +0.1 38,779 + SO0033 0.66 -0.3 38,787 - SO0033 0.67 -2.0 38,787 - SO0033 0.67 +2.3 38,787 - SO0033 0.67 +0.4 38,787 - SO0033 0.67 -0.2 38,831 + SO0033 0.71 +0.3 38,836 + SO0033 0.71 +0.1 38,859 + SO0033 0.73 -0.2 38,862 - SO0033 0.74 +0.3 38,877 - SO0033 0.75 +1.4 38,882 - SO0033 0.75 +1.9 38,977 - SO0033 0.84 +0.4 38,992 + SO0033 0.85 -2.5 39,153 - +0.6 39,207 + +0.6 39,257 + +0.4 39,265 - -0.2 39,362 + SO0034 0.12 +1.6 39,408 + SO0034 0.16 -0.4 39,408 + SO0034 0.16 -1.0 39,416 - SO0034 0.17 -0.0 39,416 - SO0034 0.17 -1.7 39,416 - SO0034 0.17 -0.1 39,421 - SO0034 0.17 -0.4 39,470 + SO0034 0.22 -3.3 39,615 - SO0034 0.36 +3.1 39,616 - SO0034 0.37 -1.1 39,625 + SO0034 0.37 +1.3 39,625 - SO0034 0.37 -0.7 39,625 - SO0034 0.37 -0.0 39,633 - SO0034 0.38 +1.2 39,633 - SO0034 0.38 +0.2 39,633 - SO0034 0.38 -3.1 39,649 + SO0034 0.40 -0.3 39,661 + SO0034 0.41 -1.5 39,737 + SO0034 0.48 -1.5 39,763 + SO0034 0.51 -2.4 39,880 - SO0034 0.63 +0.7 40,061 + SO0034 0.80 +0.1
Or see this region's nucleotide sequence