Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0013 and glyS are separated by 68 nucleotides glyS and glyQ are separated by 9 nucleotides
SO0013: SO0013 - hypothetical protein (NCBI ptt file), at 14,311 to 15,111
SO0013
SO0014: glyS - glycyl-tRNA synthetase, beta subunit (NCBI ptt file), at 15,180 to 17,246
glyS
SO0015: glyQ - glycyl-tRNA synthetase, alpha subunit (NCBI ptt file), at 17,256 to 18,161
glyQ
Position (kb) 15
16
17
18 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 14.219 kb on + strand at 14.224 kb on + strand at 14.227 kb on - strand at 14.294 kb on + strand at 14.294 kb on + strand at 14.295 kb on + strand at 14.295 kb on - strand at 14.306 kb on + strand at 14.328 kb on + strand at 14.328 kb on + strand at 14.328 kb on + strand at 14.328 kb on + strand at 14.328 kb on + strand at 14.336 kb on - strand at 14.336 kb on - strand at 14.336 kb on - strand at 14.336 kb on - strand at 14.383 kb on + strand at 14.410 kb on + strand, within SO0013 at 14.463 kb on + strand, within SO0013 at 14.468 kb on + strand, within SO0013 at 14.468 kb on + strand, within SO0013 at 14.468 kb on + strand, within SO0013 at 14.468 kb on + strand, within SO0013 at 14.468 kb on + strand, within SO0013 at 14.476 kb on - strand, within SO0013 at 14.476 kb on - strand, within SO0013 at 14.476 kb on - strand, within SO0013 at 14.487 kb on + strand, within SO0013 at 14.531 kb on + strand, within SO0013 at 14.531 kb on + strand, within SO0013 at 14.531 kb on + strand, within SO0013 at 14.531 kb on + strand, within SO0013 at 14.539 kb on - strand, within SO0013 at 14.539 kb on - strand, within SO0013 at 14.551 kb on - strand, within SO0013 at 14.802 kb on - strand, within SO0013 at 14.855 kb on + strand, within SO0013 at 14.855 kb on + strand, within SO0013 at 14.855 kb on + strand, within SO0013 at 14.855 kb on + strand, within SO0013 at 14.863 kb on - strand, within SO0013 at 14.863 kb on - strand, within SO0013 at 14.863 kb on - strand, within SO0013 at 14.962 kb on + strand, within SO0013 at 14.962 kb on + strand, within SO0013 at 15.054 kb on + strand at 15.054 kb on + strand at 15.111 kb on - strand at 15.113 kb on - strand at 18.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 14,219 + +0.2 14,224 + +2.5 14,227 - +2.5 14,294 + +2.7 14,294 + -0.7 14,295 + -1.3 14,295 - +1.6 14,306 + +0.6 14,328 + +0.0 14,328 + +0.0 14,328 + -0.3 14,328 + +1.7 14,328 + +2.4 14,336 - -3.1 14,336 - +1.3 14,336 - -0.4 14,336 - -0.9 14,383 + +1.7 14,410 + SO0013 0.12 -0.6 14,463 + SO0013 0.19 -1.3 14,468 + SO0013 0.20 -2.5 14,468 + SO0013 0.20 +1.1 14,468 + SO0013 0.20 +1.7 14,468 + SO0013 0.20 +1.6 14,468 + SO0013 0.20 +0.0 14,476 - SO0013 0.21 +0.9 14,476 - SO0013 0.21 +1.4 14,476 - SO0013 0.21 +2.4 14,487 + SO0013 0.22 +0.9 14,531 + SO0013 0.27 -0.5 14,531 + SO0013 0.27 +1.5 14,531 + SO0013 0.27 -0.3 14,531 + SO0013 0.27 +0.1 14,539 - SO0013 0.28 +0.7 14,539 - SO0013 0.28 -0.6 14,551 - SO0013 0.30 -1.0 14,802 - SO0013 0.61 +1.8 14,855 + SO0013 0.68 +1.7 14,855 + SO0013 0.68 +0.6 14,855 + SO0013 0.68 +0.0 14,855 + SO0013 0.68 +0.1 14,863 - SO0013 0.69 +1.8 14,863 - SO0013 0.69 +3.6 14,863 - SO0013 0.69 +4.3 14,962 + SO0013 0.81 +0.5 14,962 + SO0013 0.81 +0.3 15,054 + +0.3 15,054 + +1.6 15,111 - +1.1 15,113 - -0.1 18,212 - +1.7
Or see this region's nucleotide sequence