Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3079 and Dsui_3080 are separated by 145 nucleotides Dsui_3080 and Dsui_3081 are separated by 141 nucleotides Dsui_3081 and Dsui_3082 are separated by 98 nucleotides
Dsui_3079: Dsui_3079 - serine/threonine protein kinase, at 3,295,256 to 3,296,653
_3079
Dsui_3080: Dsui_3080 - molecular chaperone GrpE (heat shock protein), at 3,296,799 to 3,297,356
_3080
Dsui_3081: Dsui_3081 - chaperone protein DnaK, at 3,297,498 to 3,299,429
_3081
Dsui_3082: Dsui_3082 - chaperone protein DnaJ, at 3,299,528 to 3,300,667
_3082
Position (kb)
3297
3298
3299
3300 Strain fitness (log2 ratio)
-1
0
1
2 at 3296.547 kb on - strand at 3296.565 kb on - strand at 3296.567 kb on - strand at 3296.625 kb on + strand at 3296.662 kb on - strand at 3296.692 kb on + strand at 3296.704 kb on + strand at 3296.713 kb on - strand at 3296.921 kb on + strand, within Dsui_3080 at 3297.341 kb on + strand at 3297.341 kb on + strand at 3297.479 kb on + strand at 3299.008 kb on + strand, within Dsui_3081 at 3299.050 kb on + strand, within Dsui_3081 at 3299.119 kb on + strand, within Dsui_3081 at 3299.498 kb on + strand at 3299.510 kb on + strand at 3299.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 3,296,547 - -0.5 3,296,565 - +0.0 3,296,567 - +0.1 3,296,625 + -1.4 3,296,662 - -1.0 3,296,692 + +0.2 3,296,704 + +0.5 3,296,713 - +0.4 3,296,921 + Dsui_3080 0.22 +0.4 3,297,341 + +0.3 3,297,341 + -0.3 3,297,479 + -0.5 3,299,008 + Dsui_3081 0.78 +1.0 3,299,050 + Dsui_3081 0.80 +2.0 3,299,119 + Dsui_3081 0.84 +0.0 3,299,498 + -0.3 3,299,510 + +0.2 3,299,513 - +0.1
Or see this region's nucleotide sequence