Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3018 and Dsui_3019 are separated by 9 nucleotides Dsui_3019 and Dsui_3020 are separated by 22 nucleotides Dsui_3020 and Dsui_3021 are separated by 57 nucleotides Dsui_3021 and Dsui_3022 overlap by 4 nucleotides
Dsui_3018: Dsui_3018 - OAH/OAS sulfhydrylase, at 3,231,337 to 3,232,638
_3018
Dsui_3019: Dsui_3019 - putative hydrolase or acyltransferase of alpha/beta superfamily, at 3,232,648 to 3,233,433
_3019
Dsui_3020: Dsui_3020 - Protein of unknown function (DUF3124), at 3,233,456 to 3,233,968
_3020
Dsui_3021: Dsui_3021 - putative permease, DMT superfamily, at 3,234,026 to 3,234,913
_3021
Dsui_3022: Dsui_3022 - hypothetical protein, at 3,234,910 to 3,235,074
_3022
Position (kb)
3233
3234 Strain fitness (log2 ratio)
-1
0
1
2 at 3232.505 kb on - strand at 3232.515 kb on + strand at 3232.759 kb on + strand, within Dsui_3019 at 3233.019 kb on + strand, within Dsui_3019 at 3233.027 kb on - strand, within Dsui_3019 at 3233.040 kb on + strand, within Dsui_3019 at 3233.048 kb on - strand, within Dsui_3019 at 3233.048 kb on - strand, within Dsui_3019 at 3233.216 kb on + strand, within Dsui_3019 at 3233.239 kb on + strand, within Dsui_3019 at 3233.427 kb on + strand at 3233.435 kb on - strand at 3233.435 kb on - strand at 3233.493 kb on - strand at 3233.596 kb on + strand, within Dsui_3020 at 3233.663 kb on + strand, within Dsui_3020 at 3233.671 kb on - strand, within Dsui_3020 at 3233.793 kb on + strand, within Dsui_3020 at 3233.890 kb on + strand, within Dsui_3020 at 3233.914 kb on + strand, within Dsui_3020 at 3233.914 kb on + strand, within Dsui_3020 at 3234.166 kb on + strand, within Dsui_3021 at 3234.166 kb on + strand at 3234.166 kb on + strand, within Dsui_3021 at 3234.180 kb on - strand, within Dsui_3021 at 3234.217 kb on - strand, within Dsui_3021 at 3234.253 kb on + strand, within Dsui_3021 at 3234.266 kb on + strand, within Dsui_3021 at 3234.371 kb on + strand, within Dsui_3021 at 3234.383 kb on + strand at 3234.421 kb on - strand, within Dsui_3021 at 3234.421 kb on - strand, within Dsui_3021 at 3234.428 kb on + strand, within Dsui_3021 at 3234.454 kb on - strand, within Dsui_3021 at 3234.454 kb on - strand, within Dsui_3021 at 3234.586 kb on - strand at 3234.594 kb on - strand, within Dsui_3021 at 3234.610 kb on - strand, within Dsui_3021 at 3234.710 kb on - strand, within Dsui_3021 at 3234.740 kb on + strand, within Dsui_3021 at 3234.751 kb on + strand, within Dsui_3021 at 3234.772 kb on + strand, within Dsui_3021 at 3234.779 kb on + strand, within Dsui_3021 at 3234.894 kb on + strand at 3234.894 kb on + strand at 3234.902 kb on - strand at 3234.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 3,232,505 - +0.1 3,232,515 + +0.1 3,232,759 + Dsui_3019 0.14 -0.2 3,233,019 + Dsui_3019 0.47 +0.7 3,233,027 - Dsui_3019 0.48 -1.7 3,233,040 + Dsui_3019 0.50 -0.4 3,233,048 - Dsui_3019 0.51 -0.2 3,233,048 - Dsui_3019 0.51 -0.3 3,233,216 + Dsui_3019 0.72 -0.3 3,233,239 + Dsui_3019 0.75 -0.2 3,233,427 + -0.9 3,233,435 - +0.2 3,233,435 - -0.4 3,233,493 - -0.2 3,233,596 + Dsui_3020 0.27 -1.2 3,233,663 + Dsui_3020 0.40 -1.0 3,233,671 - Dsui_3020 0.42 +0.0 3,233,793 + Dsui_3020 0.66 -0.4 3,233,890 + Dsui_3020 0.85 -0.7 3,233,914 + Dsui_3020 0.89 -1.1 3,233,914 + Dsui_3020 0.89 +0.2 3,234,166 + Dsui_3021 0.16 +0.0 3,234,166 + -0.5 3,234,166 + Dsui_3021 0.16 +0.0 3,234,180 - Dsui_3021 0.17 -0.3 3,234,217 - Dsui_3021 0.22 -0.1 3,234,253 + Dsui_3021 0.26 +0.1 3,234,266 + Dsui_3021 0.27 +0.6 3,234,371 + Dsui_3021 0.39 -0.9 3,234,383 + +0.3 3,234,421 - Dsui_3021 0.44 -0.3 3,234,421 - Dsui_3021 0.44 -0.3 3,234,428 + Dsui_3021 0.45 -0.3 3,234,454 - Dsui_3021 0.48 +0.1 3,234,454 - Dsui_3021 0.48 -0.2 3,234,586 - -0.5 3,234,594 - Dsui_3021 0.64 -0.2 3,234,610 - Dsui_3021 0.66 +0.4 3,234,710 - Dsui_3021 0.77 -0.1 3,234,740 + Dsui_3021 0.80 -0.3 3,234,751 + Dsui_3021 0.82 -0.8 3,234,772 + Dsui_3021 0.84 +0.1 3,234,779 + Dsui_3021 0.85 +0.2 3,234,894 + -0.0 3,234,894 + +2.0 3,234,902 - -0.8 3,234,902 - -0.1
Or see this region's nucleotide sequence