Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2901 and Dsui_2902 are separated by 224 nucleotides Dsui_2902 and Dsui_2903 are separated by 31 nucleotides Dsui_2903 and Dsui_2904 are separated by 83 nucleotides
Dsui_2901: Dsui_2901 - nicotinate-nucleotide pyrophosphorylase, at 3,104,578 to 3,105,462
_2901
Dsui_2902: Dsui_2902 - hypothetical protein, at 3,105,687 to 3,105,824
_2902
Dsui_2903: Dsui_2903 - methyltransferase family protein, at 3,105,856 to 3,106,476
_2903
Dsui_2904: Dsui_2904 - threonine synthase, at 3,106,560 to 3,107,999
_2904
Position (kb)
3105
3106 Strain fitness (log2 ratio)
-1
0
1
2 at 3105.511 kb on - strand at 3105.556 kb on + strand at 3105.598 kb on + strand at 3105.678 kb on + strand at 3105.772 kb on + strand, within Dsui_2902 at 3105.812 kb on + strand at 3105.817 kb on + strand at 3105.887 kb on - strand at 3105.914 kb on + strand at 3105.992 kb on - strand, within Dsui_2903 at 3106.055 kb on - strand, within Dsui_2903 at 3106.163 kb on - strand, within Dsui_2903 at 3106.178 kb on + strand, within Dsui_2903 at 3106.345 kb on + strand, within Dsui_2903 at 3106.345 kb on + strand at 3106.348 kb on - strand, within Dsui_2903 at 3106.407 kb on - strand, within Dsui_2903 at 3106.444 kb on - strand at 3106.478 kb on - strand at 3106.549 kb on + strand at 3106.566 kb on - strand at 3106.572 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 3,105,511 - -0.4 3,105,556 + -0.0 3,105,598 + -0.9 3,105,678 + -0.4 3,105,772 + Dsui_2902 0.62 -0.5 3,105,812 + -0.2 3,105,817 + -0.1 3,105,887 - +0.3 3,105,914 + +0.4 3,105,992 - Dsui_2903 0.22 -0.3 3,106,055 - Dsui_2903 0.32 -0.6 3,106,163 - Dsui_2903 0.49 +1.2 3,106,178 + Dsui_2903 0.52 +0.3 3,106,345 + Dsui_2903 0.79 +0.6 3,106,345 + +2.0 3,106,348 - Dsui_2903 0.79 +0.4 3,106,407 - Dsui_2903 0.89 -0.2 3,106,444 - +0.6 3,106,478 - -0.6 3,106,549 + +0.4 3,106,566 - -0.0 3,106,572 - +0.2
Or see this region's nucleotide sequence