Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2718 and Dsui_2719 are separated by 186 nucleotides Dsui_2719 and Dsui_2720 are separated by 164 nucleotides Dsui_2720 and Dsui_2721 are separated by 7 nucleotides Dsui_2721 and Dsui_2722 are separated by 6 nucleotides
Dsui_2718: Dsui_2718 - polyribonucleotide nucleotidyltransferase, at 2,872,066 to 2,874,189
_2718
Dsui_2719: Dsui_2719 - ribosomal protein S15, at 2,874,376 to 2,874,645
_2719
Dsui_2720: Dsui_2720 - tRNA pseudouridine 55 synthase, at 2,874,810 to 2,875,712
_2720
Dsui_2721: Dsui_2721 - ribosome-binding factor A, at 2,875,720 to 2,876,133
_2721
Dsui_2722: Dsui_2722 - translation initiation factor IF-2, at 2,876,140 to 2,878,932
_2722
Position (kb)
2874
2875
2876 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2874.240 kb on + strand at 2874.305 kb on - strand at 2874.334 kb on - strand at 2874.807 kb on + strand at 2874.814 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.815 kb on - strand at 2874.852 kb on - strand at 2874.869 kb on + strand at 2874.947 kb on + strand, within Dsui_2720 at 2874.950 kb on - strand, within Dsui_2720 at 2875.037 kb on - strand, within Dsui_2720 at 2875.063 kb on + strand, within Dsui_2720 at 2875.063 kb on + strand, within Dsui_2720 at 2875.110 kb on + strand, within Dsui_2720 at 2875.137 kb on + strand at 2875.145 kb on - strand, within Dsui_2720 at 2875.166 kb on + strand, within Dsui_2720 at 2875.166 kb on + strand, within Dsui_2720 at 2875.399 kb on + strand, within Dsui_2720 at 2875.483 kb on + strand, within Dsui_2720 at 2875.662 kb on + strand at 2875.731 kb on - strand at 2875.815 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 2,874,240 + +0.4 2,874,305 - -0.5 2,874,334 - -0.2 2,874,807 + +1.3 2,874,814 - +0.4 2,874,815 - -0.7 2,874,815 - +0.1 2,874,815 - -0.6 2,874,815 - +0.1 2,874,852 - -0.2 2,874,869 + -0.7 2,874,947 + Dsui_2720 0.15 -0.9 2,874,950 - Dsui_2720 0.16 +0.0 2,875,037 - Dsui_2720 0.25 -2.1 2,875,063 + Dsui_2720 0.28 +2.6 2,875,063 + Dsui_2720 0.28 +1.0 2,875,110 + Dsui_2720 0.33 -0.6 2,875,137 + -0.0 2,875,145 - Dsui_2720 0.37 +0.3 2,875,166 + Dsui_2720 0.39 -0.4 2,875,166 + Dsui_2720 0.39 +0.2 2,875,399 + Dsui_2720 0.65 -0.4 2,875,483 + Dsui_2720 0.75 -2.5 2,875,662 + +0.0 2,875,731 - +0.5 2,875,815 + +1.0
Or see this region's nucleotide sequence