Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2378 and Dsui_2379 are separated by 78 nucleotides Dsui_2379 and Dsui_2380 are separated by 27 nucleotides Dsui_2380 and Dsui_2381 are separated by 19 nucleotides
Dsui_2378: Dsui_2378 - hypothetical protein, at 2,527,137 to 2,527,454
_2378
Dsui_2379: Dsui_2379 - phosphoserine phosphatase SerB, at 2,527,533 to 2,528,375
_2379
Dsui_2380: Dsui_2380 - isoleucine patch superfamily enzyme, carbonic anhydrase/acetyltransferase, at 2,528,403 to 2,528,945
_2380
Dsui_2381: Dsui_2381 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 2,528,965 to 2,530,041
_2381
Position (kb)
2528
2529 Strain fitness (log2 ratio)
-1
0
1 at 2527.487 kb on + strand at 2527.509 kb on - strand at 2528.402 kb on - strand at 2528.402 kb on - strand at 2528.549 kb on - strand, within Dsui_2380 at 2528.832 kb on - strand, within Dsui_2380 at 2528.879 kb on - strand, within Dsui_2380 at 2528.928 kb on - strand at 2528.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 2,527,487 + -0.1 2,527,509 - +0.4 2,528,402 - +0.2 2,528,402 - +0.2 2,528,549 - Dsui_2380 0.27 -0.3 2,528,832 - Dsui_2380 0.79 -0.4 2,528,879 - Dsui_2380 0.88 -1.6 2,528,928 - -0.1 2,528,935 - -0.0
Or see this region's nucleotide sequence