Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1686 and Dsui_1687 are separated by 74 nucleotides Dsui_1687 and Dsui_1688 are separated by 192 nucleotides Dsui_1688 and Dsui_1689 are separated by 18 nucleotides
Dsui_1686: Dsui_1686 - NAD+ synthetase, at 1,773,136 to 1,774,761
_1686
Dsui_1687: Dsui_1687 - nitrogen regulatory protein PII, at 1,774,836 to 1,775,174
_1687
Dsui_1688: Dsui_1688 - hypothetical protein, at 1,775,367 to 1,776,413
_1688
Dsui_1689: Dsui_1689 - thioredoxin-disulfide reductase, at 1,776,432 to 1,777,391
_1689
Position (kb)
1775
1776
1777 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1774.740 kb on + strand at 1774.807 kb on - strand at 1774.851 kb on + strand at 1774.873 kb on - strand at 1774.883 kb on + strand, within Dsui_1687 at 1774.939 kb on - strand, within Dsui_1687 at 1775.014 kb on + strand, within Dsui_1687 at 1775.022 kb on - strand, within Dsui_1687 at 1775.074 kb on - strand, within Dsui_1687 at 1775.246 kb on - strand at 1775.272 kb on + strand at 1775.363 kb on + strand at 1775.541 kb on + strand at 1775.541 kb on + strand at 1775.627 kb on - strand, within Dsui_1688 at 1775.980 kb on + strand at 1776.077 kb on + strand, within Dsui_1688 at 1776.164 kb on - strand, within Dsui_1688 at 1776.212 kb on - strand, within Dsui_1688 at 1776.322 kb on + strand at 1776.322 kb on + strand at 1776.438 kb on - strand at 1776.440 kb on + strand at 1777.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 1,774,740 + -0.4 1,774,807 - +0.6 1,774,851 + +0.1 1,774,873 - +3.4 1,774,883 + Dsui_1687 0.14 -0.3 1,774,939 - Dsui_1687 0.30 +0.3 1,775,014 + Dsui_1687 0.53 -0.2 1,775,022 - Dsui_1687 0.55 +0.4 1,775,074 - Dsui_1687 0.70 -0.3 1,775,246 - +0.7 1,775,272 + -0.8 1,775,363 + -0.3 1,775,541 + +0.3 1,775,541 + +1.5 1,775,627 - Dsui_1688 0.25 -0.0 1,775,980 + -1.6 1,776,077 + Dsui_1688 0.68 +0.0 1,776,164 - Dsui_1688 0.76 -0.5 1,776,212 - Dsui_1688 0.81 -0.5 1,776,322 + +1.0 1,776,322 + -2.2 1,776,438 - -1.1 1,776,440 + +0.2 1,777,401 - +0.0
Or see this region's nucleotide sequence