Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1660 and Dsui_1661 overlap by 1 nucleotides Dsui_1661 and Dsui_1662 are separated by 119 nucleotides Dsui_1662 and Dsui_1663 are separated by 162 nucleotides
Dsui_1660: Dsui_1660 - outer membrane cobalamin receptor protein, at 1,743,236 to 1,745,314
_1660
Dsui_1661: Dsui_1661 - hypothetical protein, at 1,745,314 to 1,745,790
_1661
Dsui_1662: Dsui_1662 - Oxidoreductase NAD-binding domain/Flavodoxin, at 1,745,910 to 1,747,262
_1662
Dsui_1663: Dsui_1663 - ABC-type Co2+ transport system, periplasmic component, at 1,747,425 to 1,748,228
_1663
Position (kb)
1745
1746
1747
1748 Strain fitness (log2 ratio)
-2
-1
0
1 at 1745.037 kb on + strand, within Dsui_1660 at 1745.173 kb on - strand at 1745.213 kb on + strand at 1745.327 kb on + strand at 1745.335 kb on - strand at 1745.346 kb on - strand at 1745.377 kb on + strand, within Dsui_1661 at 1745.481 kb on - strand at 1745.493 kb on - strand, within Dsui_1661 at 1745.532 kb on + strand, within Dsui_1661 at 1745.563 kb on - strand, within Dsui_1661 at 1745.574 kb on + strand, within Dsui_1661 at 1745.574 kb on - strand, within Dsui_1661 at 1745.594 kb on + strand, within Dsui_1661 at 1745.599 kb on + strand at 1745.602 kb on - strand at 1745.641 kb on - strand, within Dsui_1661 at 1745.829 kb on - strand at 1745.829 kb on - strand at 1745.839 kb on + strand at 1745.909 kb on - strand at 1745.952 kb on + strand at 1745.960 kb on - strand at 1745.986 kb on - strand at 1746.057 kb on + strand, within Dsui_1662 at 1746.088 kb on - strand, within Dsui_1662 at 1746.134 kb on + strand, within Dsui_1662 at 1746.267 kb on - strand, within Dsui_1662 at 1746.271 kb on + strand, within Dsui_1662 at 1746.308 kb on + strand at 1746.436 kb on + strand at 1746.805 kb on - strand, within Dsui_1662 at 1746.891 kb on + strand, within Dsui_1662 at 1746.891 kb on + strand at 1746.907 kb on + strand, within Dsui_1662 at 1747.028 kb on + strand at 1747.029 kb on + strand at 1747.047 kb on - strand, within Dsui_1662 at 1747.196 kb on - strand at 1747.326 kb on - strand at 1747.642 kb on - strand, within Dsui_1663 at 1747.690 kb on + strand, within Dsui_1663 at 1747.845 kb on + strand at 1747.880 kb on - strand, within Dsui_1663 at 1747.892 kb on + strand, within Dsui_1663 at 1747.900 kb on - strand, within Dsui_1663 at 1747.939 kb on + strand, within Dsui_1663 at 1747.947 kb on - strand, within Dsui_1663 at 1748.093 kb on - strand, within Dsui_1663 at 1748.116 kb on + strand at 1748.116 kb on + strand at 1748.116 kb on + strand, within Dsui_1663
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 1,745,037 + Dsui_1660 0.87 -0.5 1,745,173 - +0.2 1,745,213 + -0.2 1,745,327 + -0.1 1,745,335 - -1.6 1,745,346 - +1.0 1,745,377 + Dsui_1661 0.13 -0.3 1,745,481 - -1.1 1,745,493 - Dsui_1661 0.38 +0.7 1,745,532 + Dsui_1661 0.46 +0.1 1,745,563 - Dsui_1661 0.52 -0.4 1,745,574 + Dsui_1661 0.55 +0.2 1,745,574 - Dsui_1661 0.55 -0.2 1,745,594 + Dsui_1661 0.59 -0.4 1,745,599 + +0.2 1,745,602 - -0.6 1,745,641 - Dsui_1661 0.69 +0.3 1,745,829 - -0.6 1,745,829 - -0.0 1,745,839 + -1.0 1,745,909 - +1.1 1,745,952 + +0.4 1,745,960 - -0.5 1,745,986 - +0.3 1,746,057 + Dsui_1662 0.11 -1.1 1,746,088 - Dsui_1662 0.13 -0.6 1,746,134 + Dsui_1662 0.17 -1.0 1,746,267 - Dsui_1662 0.26 -2.5 1,746,271 + Dsui_1662 0.27 -0.1 1,746,308 + +0.4 1,746,436 + +1.0 1,746,805 - Dsui_1662 0.66 -0.2 1,746,891 + Dsui_1662 0.73 -0.6 1,746,891 + +0.2 1,746,907 + Dsui_1662 0.74 -0.3 1,747,028 + -0.5 1,747,029 + -0.2 1,747,047 - Dsui_1662 0.84 -1.6 1,747,196 - +0.4 1,747,326 - -0.8 1,747,642 - Dsui_1663 0.27 +0.1 1,747,690 + Dsui_1663 0.33 -0.2 1,747,845 + -0.2 1,747,880 - Dsui_1663 0.57 +0.0 1,747,892 + Dsui_1663 0.58 -0.1 1,747,900 - Dsui_1663 0.59 -0.3 1,747,939 + Dsui_1663 0.64 -0.9 1,747,947 - Dsui_1663 0.65 +0.3 1,748,093 - Dsui_1663 0.83 +0.8 1,748,116 + -2.1 1,748,116 + -1.5 1,748,116 + Dsui_1663 0.86 -0.4
Or see this region's nucleotide sequence