Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1481 and Dsui_1482 are separated by 711 nucleotides Dsui_1482 and Dsui_1483 are separated by 42 nucleotides
Dsui_1481: Dsui_1481 - hypothetical protein, at 1,545,389 to 1,546,507
_1481
Dsui_1482: Dsui_1482 - hypothetical protein, at 1,547,219 to 1,547,503
_1482
Dsui_1483: Dsui_1483 - putative transcription regulator containing HTH domain, at 1,547,546 to 1,548,760
_1483
Position (kb)
1547
1548 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1546.272 kb on - strand, within Dsui_1481 at 1546.335 kb on + strand, within Dsui_1481 at 1546.468 kb on - strand at 1546.530 kb on + strand at 1546.538 kb on - strand at 1546.588 kb on - strand at 1546.645 kb on + strand at 1546.646 kb on - strand at 1546.650 kb on + strand at 1546.652 kb on + strand at 1546.655 kb on - strand at 1546.660 kb on - strand at 1546.711 kb on - strand at 1546.773 kb on + strand at 1546.776 kb on - strand at 1546.776 kb on - strand at 1546.960 kb on + strand at 1546.960 kb on + strand at 1546.999 kb on - strand at 1547.037 kb on + strand at 1547.108 kb on + strand at 1547.241 kb on + strand at 1547.445 kb on + strand, within Dsui_1482 at 1547.453 kb on - strand, within Dsui_1482 at 1547.453 kb on - strand, within Dsui_1482 at 1547.468 kb on - strand, within Dsui_1482 at 1547.507 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 1,546,272 - Dsui_1481 0.79 +0.3 1,546,335 + Dsui_1481 0.85 -0.1 1,546,468 - -0.0 1,546,530 + -1.2 1,546,538 - +1.2 1,546,588 - -0.5 1,546,645 + -0.2 1,546,646 - +0.6 1,546,650 + +0.5 1,546,652 + +0.8 1,546,655 - -0.6 1,546,660 - -0.0 1,546,711 - -0.2 1,546,773 + +0.1 1,546,776 - +1.0 1,546,776 - -0.4 1,546,960 + -0.5 1,546,960 + -0.3 1,546,999 - +2.0 1,547,037 + -0.7 1,547,108 + -0.1 1,547,241 + +0.1 1,547,445 + Dsui_1482 0.79 -1.7 1,547,453 - Dsui_1482 0.82 -0.2 1,547,453 - Dsui_1482 0.82 +0.7 1,547,468 - Dsui_1482 0.87 -0.6 1,547,507 + -3.0
Or see this region's nucleotide sequence